Impact of collagen crosslinking on the second harmonic generation signal and the fluorescence lifetime of collagen autofluorescence

Skin Research and Technology

Volumn 18, Issue 2, 2012, Pages 168-179

  Lutz, Vivien ^a,b   Sattler, Martin ^a   Gallinat, Stefan ^a   Wenck, Horst ^a   Poertner, Ralf ^b   Fischer, Frank ^a  

^a BEIERSDORF AG   (Germany)

^b HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Collagen crosslinking; Dermal photodamage; Fluorescence lifetime; Multiphoton laser scanning microscopy; Second harmonic generation

Indexed keywords

AUTOFLUORESCENCE; COLLAGEN CROSSLINKING; COLLAGEN FIBER; COLLAGEN FIBRILS; COLLAGEN MOLECULES; COLLAGEN SYNTHESIS; CROSSLINKS; FLUORESCENCE LIFETIME; FLUORESCENCE LIFETIMES; FLUORESCENT MOLECULES; IN-VITRO; IN-VIVO MEASUREMENT; MICROENVIRONMENTS; MULTIPHOTON LASER SCANNING MICROSCOPY; MULTIPHOTON LASERS; PHOTO-DAMAGE; PHOTO-DAMAGED SKIN; SHG SIGNALS; STRUCTURAL PROTEINS; TWO PARAMETER;

FLUORESCENCE; HARMONIC GENERATION; MOLECULES; NONLINEAR OPTICS;

COLLAGEN;

COLLAGEN;

ARTICLE; AUTOFLUORESCENCE; COLLAGEN SYNTHESIS; CROSS LINKING; IN VITRO STUDY; MICROENVIRONMENT; MULTIPHOTON MICROSCOPY; PROTEIN STRUCTURE; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; ANIMALS; CELLS, CULTURED; COLLAGEN TYPE I; CROSS-LINKING REAGENTS; DERMIS; FIBROBLASTS; FLUORESCENCE; HUMANS; INFANT, NEWBORN; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, ELECTRON, TRANSMISSION; MOLECULAR SEQUENCE DATA; RATS; SIGNAL TRANSDUCTION; SKIN AGING;

EID: 84859380793     PISSN: 0909752X     EISSN: 16000846     Source Type: Journal    
DOI: 10.1111/j.1600-0846.2011.00549.x     Document Type: Article

References (20)

1. Wulf HC, Möller JS, Kobayasi T, Gniadecki R. Skin aging and natural photoprotection. Micron 2004; 35: 185-191.
2. Weedon D. Skin pathology. In: D. Weedon, ed. Weedon's skin pathology (3rd edn). Oxford, UK: Churchill Livingstone Elsevier 2010: 327-328.
3. Buehler MJ. Nanomechanics of collagen fibrils under varying cross-link densities: atomistic and continuum studies. J Mech Behav Biomed Mater 2008; 1: 59-67.
4. Yamauchi M, Prisayanh P, Haque Z, Woodley DT. Collagen cross-linking in sun-exposed and unexposed sites of aged human skin. J Invest Dermatol 1991; 97: 938-941.
5. Cox G, Kable E, Jones A, Fraser I, Manconi F, Gorrell MD.3-Dimensional imaging of collagen using second harmonic generation. J Struct Biol 2003; 141: 53-62.
6. Fischer F, Achterberg V, März Aet al. Folic acid and creatine improve the firmness of human skin in vivo. J Cosmet Dermatol 2011; 10: 34-42.
7. Harlow CR, Rae M, Davidson L, Trackman PC, Hillier SG. Lysyl oxidase gene expression and enzyme activity in the rat ovary: regulation by follicle stimulating hormone, androgen, and transforming growth factor-b superfamily members in vitro. Endocrinology 2003; 144: 154-162.
8. Wu J, Reinhardt DP, Batmunkh C, Lindenmaier W, Far RK, Notbohm H, Hunzelmann N, Brinckmann J. Functional diversity of lysyl hydroxylase 2 in collagen synthesis of human dermal fibroblasts. Exp Cell Res 2006; 312: 3485-3494.
9. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using realtime quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001; 25: 402-408.
10. Venable JH, Coggeshall R.A simplified lead citrate stain for use in electron microscopy. J Cell Biol 1965; 25: 407-408.
11. Fischer F, Volkmer B, Puschmann Set al. Assessing the risk of skin damage due to femtosecond laser irradiation. J Biophotonics 2008; 1: 470-477.
12. Becker W. Advanced time-correlated single photon counting techniques. Berlin: Springer-Verlag, 2005.
13. Becker W. Recording the instrument response function of a multiphoton FLIM system. Application note, 2008. Available at (accessed March 14, 2011).
14. Nakabayashi T, Wang HP, Kinjo M, Ohta N. Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements. Photochem Photobiol Sci 2008; 7: 668-670.
15. Varani J, Spearman D, Perone P, Fligiel SE, Datta SC, Wang ZQ, Shao Y, Kang S, Fisher GJ, Voorhees JJ. Inhibition of type I procollagen synthesis by damaged collagen in photoaged skin and by collagenase-degraded collagen in vitro. Am J Pathol 2001; 3: 931-942.
16. Wong M, Siegrist M, Gaschen V, Park Y, Graber W, Studer D. Collagen fibrillogenesis by chondrocytes in alginate. Tissue Eng 2002; 8: 979-987.
17. Neame PJ, Kay CJ, McQuillan DJ, Beales PM, Hassell JR. Independent modulation of collagen fibrillogenesis by decorin and lumican. Cell Mol Life Sci 2000; 57: 859-863.
18. Stoller P, Celliers PM, Reiser KM, Rubenchik AM. Quantitative second-harmonic generation microscopy in collagen. Appl Opt 2003; 42: 5209-5219.
19. Kadler KE, Holmes DF, Trotter JA, Chapman JA. Collagen fibril formation. Biochem J 1996; 316: 1-11.
20. vandeVen M, Ameloot M, Valeur B, Boens N. Pitfalls and their remedies in time-resolved fluorescence spectroscopy and microscopy. J Fluoresc 2005; 15: 377-413.