Single-molecule assays reveal that RNA localization signals regulate dyneing-dynactin copy number on individual transcript cargoes

Nature Cell Biology

Volumn 14, Issue 4, 2012, Pages 416-423

  Amrute Nayak, Mamta ^a   Bullock, Simon L ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DYNACTIN; DYNEIN ADENOSINE TRIPHOSPHATASE; MESSENGER RNA; RIBONUCLEOPROTEIN; RNA;

ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; EMBRYO; GENE DOSAGE; GENE EXPRESSION; IN VITRO STUDY; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; RNA ANALYSIS; RNA TRANSCRIPTION; RNA TRANSPORT; SIGNAL TRANSDUCTION;

ANIMALS; DROSOPHILA MELANOGASTER; DYNEINS; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; RIBONUCLEOPROTEINS; RNA TRANSPORT; RNA, MESSENGER; SIGNAL TRANSDUCTION; TUBULIN;

EID: 84859297558     PISSN: 14657392     EISSN: 14764679     Source Type: Journal    
DOI: 10.1038/ncb2446     Document Type: Article

References (18)

1. Bullock, S.L. & Ish-Horowicz, D. Conserved signals and machinery for RNA transport in Drosophila oogenesis and embryogenesis. Nature 414, 611-616 (2001). (Pubitemid 33151251)
2. Edgar, B.A., Weir, M.P., Schubiger, G. & Kornberg, T. Repression and turnover pattern fushi tarazu RNA in the early Drosophila embryo. Cell 47, 747-754 (1986). (Pubitemid 17011418)
3. Bullock, S.L., Ringel, I., Ish-Horowicz, D. & Lukavsky, P.J. A'-form RNA helices are required for cytoplasmic mRNA transport in Drosophila. Nat. Struct. Mol. Biol. 17, 703-709 (2010).
4. Serano, T.L. & Cohen, R.S. A small predicted stem-loop structure mediates oocyte localization of Drosophila K10 mRNA. Development 121, 3809-3818 (1995). (Pubitemid 3000455)
5. Cohen, R.S., Zhang, S. & Dollar, G.L. The positional, structural, and sequence requirements of the Drosophila TLS RNA localization element. RNA 11, 1017-1029 (2005). (Pubitemid 40994183)
6. Mallik, R., Petrov, D., Lex, S.A., King, S.J. & Gross, S.P. Building complexity: an in vitro study of cytoplasmic Dynein with in vivo implications. Curr. Biol. 15, 2075-2085 (2005). (Pubitemid 41739739)
7. Ross, J.L., Wallace, K., Shuman, H., Goldman, Y.E. & Holzbaur, E.L. Processive bidirectional motion of dynein-dynactin complexes in vitro. Nat. Cell Biol. 8, 562-570 (2006). (Pubitemid 43827348)
8. Srisawat, C. & Engelke, D.R. Streptavidin aptamers: affinity tags for the study of RNAs and ribonucleoproteins. RNA 7, 632-641 (2001). (Pubitemid 32416820)
9. Vale, R.D. et al. Direct observation of single kinesin molecules moving along microtubules. Nature 380, 451-453 (1996). (Pubitemid 26102723)
10. Coy, D.L., Hancock, W.O., Wagenbach, M. & Howard, J. Kinesin's tail domain is an inhibitory regulator of the motor domain. Nat. Cell Biol. 1, 288-292 (1999). (Pubitemid 129495779)
11. Kardon, J.R., Reck-Peterson, S.L. & Vale, R.D. Regulation of the processivity and intracellular localization of Saccharomyces cerevisiae dynein by dynactin. Proc. Natl. Acad. Sci. USA 106, 5669-5674 (2009).
12. Jain, A. et al. Probing cellular protein complexes using single-molecule pull-down. Nature 473, 484-488 (2011).
13. Das, S.K., Darshi, M., Cheley, S., Wallace, M.I. & Bayley, H. Membrane protein stoichiometry determined from the step-wise photobleaching of dye-labelled subunits. Chembiochem 8, 994-999 (2007). (Pubitemid 47183616)
14. Shu, D., Zhang, H., Jin, J. & Guo, P. Counting of six pRNAs of phi29 DNA-packaging motor with customized single-molecule dual-view system. EMBO J 26, 527-537 (2007). (Pubitemid 46160952)
15. Schuster, M. et al. Controlled and stochastic retention concentrates dynein at microtubule ends to keep endosomes on track. EMBO J 30, 652-664 (2011).
16. Schafer, D.A., Gill, S.R., Cooper, J.A., Heuser, J.E. & Schroer, T.A. Ultrastructural analysis of the dynactin complex: an actin-related protein is a component of a filament that resembles F-actin. J. Cell Biol. 126, 403-412 (1994). (Pubitemid 24226256)
17. Hendricks, A.G. et al. Motor Coordination via a Tug-of-War Mechanism Drives Bidirectional Vesicle Transport. Curr. Biol. 20, 697-702 (2010).
18. King, S.J., Bonilla, M., Rodgers, M.E. & Schroer, T.A. Subunit organization in cytoplasmic dynein subcomplexes. Protein Sci. 11, 1239-1250 (2002). (Pubitemid 34441241)