Purification and characterization of methyl parathion hydrolase from Burkholderia cepacia capable of degrading organophosphate insecticides

World Journal of Microbiology and Biotechnology

Volumn 28, Issue 4, 2012, Pages 1739-1746

  Ekkhunnatham, Anirut ^a   Jongsareejit, Boonsri ^a   Yamkunthong, Wanphen ^a   Wichitwechkarn, Jesdawan ^a  

^a SILPAKORN UNIVERSITY   (Thailand)

Author keywords

Methyl parathion hydrolase; mpdB gene; MPH; Organophosphate hydrolase

Indexed keywords

AFFINITY COLUMN; BURKHOLDERIA CEPACIA; CROSS REACTIVITY; DITHIOTHREITOL; FUSION PROTEINS; METHYL PARATHION HYDROLASE; MPH; NATIVE ENZYMES; OPTIMAL TEMPERATURE; ORGANOPHOSPHATE COMPOUNDS; ORGANOPHOSPHATE HYDROLASE; ORGANOPHOSPHATE INSECTICIDES; POTENTIAL APPLICATIONS; PURIFIED ENZYME; RECOMBINANT PLASMID; SEPHADEX G100; SINGLE-STEP; SODIUM DODECYL SULFATE; SPECIFIC ACTIVITY; THAILAND;

CADMIUM COMPOUNDS; CLONING; DETOXIFICATION; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENES; HYDROLASES; ION EXCHANGE; MOLECULAR MASS; PURIFICATION; SODIUM SULFATE;

ENZYME ACTIVITY;

BACTERIAL DNA; ENZYME ACTIVATOR; ENZYME INHIBITOR; INSECTICIDE; ORGANOPHOSPHATE; PARATHION METHYL; PHOSPHATASE; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BURKHOLDERIA CEPACIA; CHEMISTRY; DNA SEQUENCE; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOELECTRIC POINT; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PH; PLASMID; TEMPERATURE; THAILAND;

BURKHOLDERIA CEPACIA; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; DNA, BACTERIAL; ENZYME ACTIVATORS; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; INSECTICIDES; ISOELECTRIC POINT; METHYL PARATHION; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; ORGANOPHOSPHATES; PHOSPHORIC MONOESTER HYDROLASES; PLASMIDS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; TEMPERATURE; THAILAND;

BURKHOLDERIA CEPACIA; ESCHERICHIA COLI;

EID: 84859268823     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-011-0985-y     Document Type: Article

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