RHD Allelic Identification Among D-Brazilian Blood Donors as a Routine Test Using Pools of DNA

Journal of Clinical Laboratory Analysis

Volumn 26, Issue 2, 2012, Pages 104-108

  Mota, Mariza ^a   Dezan, M ^a   Valgueiro, M C ^b   Sakashita, A M ^a   Kutner, J M ^a   Castilho, L ^c  

^a HOSPITAL ISRAELITA ALBERT EINSTEIN   (Brazil)

^b HEMOPE   (Brazil)

^c UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

Blood donors; D antigen; PCR; Pool of DNA; RHD alleles

Indexed keywords

DNA; RHESUS D ANTIGEN;

ALLELE; ALLOIMMUNIZATION; ARTICLE; BLOOD DONOR; BLOOD GROUP TYPING; BLOOD TRANSFUSION; EXON; FOLLOW UP; GENETIC POLYMORPHISM; HAPLOTYPE; HUMAN; INTRON; MOLECULAR TYPING; POLYMERASE CHAIN REACTION; SEROLOGY;

ALLELES; BLOOD DONORS; BRAZIL; DIAGNOSTIC TESTS, ROUTINE; DNA; FOLLOW-UP STUDIES; HUMANS; MOLECULAR TYPING; POLYMORPHISM, GENETIC; RH-HR BLOOD-GROUP SYSTEM;

EID: 84859166035     PISSN: 08878013     EISSN: 10982825     Source Type: Journal    
DOI: 10.1002/jcla.21489     Document Type: Article

References (28)

1. Westhoff CM. The Rh blood group system in review: a new face for the next decade. Transfusion 2004;44:1663-1673.
2. Wagner FF, Flegel WA. Review: the molecular basis of the Rh blood group phenotypes. Immunohematology 2004; 20:23-36.
3. Wagner FF, Frohmajer A, Flegel WA. RHD positive haplotypes in D negative Europeans. BMC Genet 2001;2-10.
4. Polin H, Danzer M, Gaszner W, Broda D, St-Louis M, Proll J, Hofer K, Gabriel C. 2009. Identification of RHD alleles with the potential of anti-D immunization among seemingly D- blood donors in Upper Austria. Transfusion 49:676-681.
5. Flegel WA, von Zabern I, Wagner FF. Six years' experience performing RHD genotyping to confirm D- red blood cell units in Germany for preventing anti-D immunizations. Transfusion 2009;49:465-471.
6. Gassner C, Doescher A, Drnovsek TD, Rozman P, Eiche RNI, Legler TJ, Lukin S, Garritsen H, Kleinrath T, Egger B, Ehling R, Kormoczi GF, Kilga-Nogler S, Schoenitzer D, Petershofen EK. Presence of RHD in serologically D-, C/E+ individuals: a European multicenter study. Transfusion 2005;45:527-538.
7. Flegel WA. Molecular genetics and clinical applications for RH (review). Transfus Apher Sci 2011;44:81-91.
8. Wagner FF, Moulds JM, Tounkara A, Kouriba B, Flegel WA. RHD allele distribution in Africans of Mali. BMC Genet 2003;4-14.
9. s gene in Brazilians with the D-negative phenotype. Braz J Med Biol Res 2002;35(5):767-773.
10. Singleton BK, Green CA, Avent ND, Martin PG, Smart E, Daka A, Narter-Olaga EG, Hawthorne LM, Daniels G. The presence of an RHD pseudogene containing a 37 base pair duplication and a nonsense mutation in Africans with the RhD-negative blood group phenotype. Blood 2000;95:12-18.
11. Qun X, Grootkerk-TaxM GHM, Maaskant-van Wijk PA, van der Schoot C E. Systemic analysis and zigosity determination of the RHD gene in a D negative Chinese Han population reveals a novel D- negative RHD gene. Vox Sang 2005;88:35-40.
12. Flegel W A, Wagner FF. Molecular biology of partial D and weak D: implications for blood bank practice. Clin Lab 2002;48:53-59.
13. Wagner FF. 1998; The RhesusBase, Department of Transfusion Medicine, Ulm, Germany: University Hospital. Accessed March 11, 2011.
14. Wagner FF, Gassner C, Muller TH, Schonitzer D, Schunter F, Flegel W. Molecular basis of weak D phenotypes. Blood 1999;93:385-393.
15. Wagner FF. Influence of Rh phenotype on the antigen density of C, c, and D. Flow cytometric study using a frozen standard red cell. Transfusion 1994;34:671-676.
16. Mota M, Fonseca NL, Rodrigues A, Kutner JM, Castilho L. Anti-D alloimmunization by weak D type 1 red blood cells with a very low antigen density. Vox Sang 2005;88:130-135.
17. Denomme GA, Dake LR, Vilensky D, Ramyar L, Judd WJ. Rh discrepancies caused by variable reactivity of partial and weak D types with different serologic techniques. Transfusion 2008;48:473-478.
18. Körmöczi GF, Gassner C, Shao CP, Uchikawa M, Legler TJ. A comprehensive analysis of DEL types: partial DEL individuals are prone to anti-D alloimmunization. Transfusion 2005;45:1561-1567.
19. Mueller TH, Wagner FF, Trockenbacher A, Eicher NI, Flegel WA, Schonitzer D, Schunter F, Gassner C. PCR screening for common weak D types shows different distributions in three Central European populations. Transfusion 2001;41:45-52.
20. Maaskant-van Wijk PA, Faas BHW, de Ruijter JAM, Overbeeke MAM, von dem Borne AEGKr, van Rhenen DJ, van der Schoot CE. Genotyping of RHD by multiplex polymerase chain reaction analysis of six RHD-specific exons. Transfusion 1998;38:1015-1021.
21. Chiu RWK, Murphy MF, Fidler C, Zee B CY, Wainscoat JS, Lo DYM. Determination of RhD zygosity: comparison of a double amplification refractory mutation system approach and a multiplex real-time quantitative PCR approach. Clin Chem 2001;47:667-672.
22. Wagner FF, Flegel W. RHD gene deletion occurred in the Rhesus box. Blood 2000;95:3662-3668.
23. Legler TJ, Maas JH, Köhler M, Wagner T, Daniels GL, Perco P, Panzer S. RHD Sequencing: a new tool for decision making on transfusion therapy and provision of Rh prophylaxis. Transf Med 2001;11:383-388.
24. Ansart-Pirenne H, Asso-Bonnet M, Le Pennec PY, Roussel M, Patereau C, Noizat-Pirenne F. RhD variants in Caucasians: consequences for checking clinically relevant alleles. Transfusion 2004;44:1282-1286.
25. Flegel WA. Blood group genotyping in Germany. Transfusion 2007;47:47S-53S.
26. Denomme GA, Wagner FF, Fernandes BJ, Li W, Flegel WA. Partial D, weak D types, and novel RHD alleles among 33, 864 multiethnic patients: implications for anti-D alloimmunization and prevention. Transfusion 2005;45:1554-1560.
27. Flegel WA. How I manage donors and patients with a weak D phenotype. Curr Opin Hematol 2006;13:476-483.
28. Garratty G. Do we need to be more concerned about weak D antigens? Transfusion 2005;45:1547-1551.