On-the-resin N-terminal modification of long synthetic peptides

Analytical Biochemistry

Volumn 424, Issue 2, 2012, Pages 137-139

  Stahl, Patrick J ^a   Cruz, Juan C ^a   Li, Yang ^b   Michael Yu, S ^a,b   Hristova, Kalina ^a  

^a Johns Hopkins University   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

High yields; N terminal peptide labeling

Indexed keywords

SYNTHETIC RESINS;

COUPLING REAGENTS; EFFICIENT PROTOCOLS; FLUORESCENT DYES; HIGH YIELD; HYDROPHOBIC PEPTIDES; LARGE AMOUNTS; N-TERMINALS; SYNTHETIC PEPTIDE;

PEPTIDES;

FLUORESCENT DYE; PEPTIDE DERIVATIVE; RESIN;

AMINO TERMINAL SEQUENCE; ARTICLE; FLUORESCENCE ANALYSIS; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN MODIFICATION; PROTEIN STRUCTURE;

EID: 84859065300     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.02.032     Document Type: Article

References (24)

1. J.R. Marks, J. Placone, K. Hristova, and W.C. Wimley Spontaneous membrane-translocating peptides by orthogonal high-throughput screening J. Am. Chem. Soc. 133 2011 8995 9004
2. D.M. Togashi, and A.G. Ryder Assessing protein-surface interactions with a series of multi-labeled BSA using fluorescence lifetime microscopy and Forster energy resonance transfer Biophys. Chem. 152 2010 55 64
3. P.J. Carter Introduction to current and future protein therapeutics: a protein engineering perspective Exp. Cell Res. 317 2011 1261 1269
4. R.J. Payne, and C.H. Wong Advances in chemical ligation strategies for the synthesis of glycopeptides and glycoproteins Chem. Commun. 46 2010 21 43
5. A. Diaz-Rodriguez, and B.G. Davis Chemical modification in the creation of novel biocatalysts Curr. Opin. Chem. Biol. 15 2011 211 219
6. L. Berrade, A.E. Garcia, and J.A. Camarero Protein microarrays: novel developments and applications Pharm. Res. 28 2011 1480 1499
7. E. Basle, N. Joubert, and M. Pucheault Protein chemical modification on endogenous amino acids Chem. Biol. 17 2010 213 227
8. E. Li, and K. Hristova Imaging FRET measurements of transmembrane helix interactions in lipid bilayers on a solid support Langmuir 20 2004 9053 9060
9. E. Li, M. You, and K. Hristova SDS-PAGE and FRET suggest weak interactions between FGFR3 TM domains in the absence of extracellular domains and ligands Biochemistry 44 2005 352 360
10. S. Schick, L.R. Chen, E. Li, J. Lin, I. Koper, and K. Hristova Assembly of the M2 tetramer is strongly modulated by lipid chain length Biophys. J. 99 2010 1810 1817
11. M. You, E. Li, W.C. Wimley, and K. Hristova FRET in liposomes: measurements of TM helix dimerization in the native bilayer environment Anal. Biochem. 340 2005 154 164
12. L. Chen, M. Merzlyakov, T. Cohen, Y. Shai, and K. Hristova Energetics of ErbB1 transmembrane domain dimerization in lipid bilayers Biophys. J. 96 2009 4622 4630
13. M. Merzlyakov, and K. Hristova Forster resonance energy transfer measurements of transmembrane helix dimerization energetics Methods Enzymol: Fluorescence Spectrosc. 450 2008 107 127
14. M. You, J. Spangler, E. Li, X. Han, P. Ghosh, and K. Hristova Effect of pathogenic cysteine mutations on FGFR3 transmembrane domain dimerization in detergents and lipid bilayers Biochemistry 46 2007 11039 11046 (Pubitemid 47502770)
15. T. Iwamoto, M. You, E. Li, J. Spangler, J.M. Tomich, and K. Hristova Synthesis and initial characterization of FGFR3 transmembrane domain: consequences of sequence modifications Biochim. Biophys. Acta 1668 2005 240 247 (Pubitemid 40298928)
16. J. Fernandez-Carneado, and E. Giralt An efficient method for the solid-phase synthesis of fluorescently labelled peptides Tetrahedron Lett. 45 2004 6079 6081 (Pubitemid 38916562)
17. F. Albericio, M. Cases, J. Alsina, S.A. Triolo, L.A. Carpino, and S.A. Kates On the use of PyAOP, a phosphonium salt derived from HOAt, in solid-phase peptide synthesis Tetrahedron Lett. 38 1997 4853 4856 (Pubitemid 27277980)
18. F. Albericio, and L.A. Carpino Coupling reagents and activation Solid-Phase Peptide Syn. 289 1997 104 126 (Pubitemid 27467245)
19. R. Fischer, O. Mader, G. Jung, and R. Brock Extending the applicability of carboxyfluorescein in solid-phase synthesis Bioconjugate Chem. 14 2003 653 660 (Pubitemid 36597377)
20. J. Baum, and B. Brodsky Folding of peptide models of collagen and misfolding in disease Cur. Opin. Struc. Biol. 9 1999 122 128 (Pubitemid 29471984)
21. A.Y. Wang, X. Mo, C.S. Chen, and S.M. Yu Facile modification of collagen directed by collagen mimetic peptides J. Am. Chem. Soc. 127 2005 4130 4131 (Pubitemid 40463012)
22. A.Y. Wang, S. Leong, Y.C. Liang, R.C.C. Huang, C.S. Chen, and S.M. Yu Immobilization of growth factors on collagen scaffolds mediated by polyanionic collagen mimetic peptides and its effect on endothelial cell morphogenesis Biomacromolecules 9 2008 2929 2936
23. A.Y. Wang, C.A. Foss, S. Leong, X. Mo, M.G. Pomper, and S.M. Yu Spatio-temporal modification of collagen scaffolds mediated by triple helical propensity Biomacromolecules 9 2008 1755 1763
24. P.J. Stahl, N.H. Romano, D. Wirtz, and S.M. Yu PEG-based hydrogels with collagen mimetic peptide-mediated and tunable physical cross-links Biomacromolecules 11 2010 2336 2344