Evaluation of physiological importance of metallothionein protein expressed by Tetrahymena cadmium metallothionein 1 (TMCd1) gene in Escherichia coli

Journal of Cellular Biochemistry

Volumn 113, Issue 5, 2012, Pages 1616-1622

  Suleman, Aliya ^a   Shakoori, Abdul Rauf ^a  

^a UNIVERSITY OF THE PUNJAB   (Pakistan)

Author keywords

CADMIUM; CADMIUM RESISTANCE GENE; HEAVY METALS; METAL UP TAKE; METALLOTHIONEIN; SITE DIRECTED MUTAGENESIS; Tetrahymena

Indexed keywords

BINDING PROTEIN; CADMIUM; METALLOTHIONEIN;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; DETOXIFICATION; ESCHERICHIA COLI; GENE; GENE SILENCING; METAL BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTOZOAL GENETICS; SITE DIRECTED MUTAGENESIS; TETRAHYMENA; TETRAHYMENA CADMIUM METALLOTHIONEIN 1 GENE;

ANIMALS; BASE SEQUENCE; CADMIUM; DNA, PROTOZOAN; ESCHERICHIA COLI; GENES, PROTOZOAN; METALLOTHIONEIN; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTOZOAN PROTEINS; RECOMBINANT FUSION PROTEINS; TETRAHYMENA;

BACTERIA (MICROORGANISMS); CILIOPHORA; ESCHERICHIA COLI; PROTOZOA; TETRAHYMENA;

EID: 84859034032     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.24030     Document Type: Article

References (16)

1. 2+ bioremediation. Biodegradation 8: 97-103. (Pubitemid 27415910)
2. Coyle P, Philcox JC, Carey LC, Rofe AM,. 2002. Metallothionein: The multipurpose protein. Cell Mol Life Sci 59: 627-640.
3. Domenech J, Bofill R, Tinti A, Torreggiani A, Atrian S, Capdevila M,. 2008. Comparative insight into the Zn(II)-, Cd(II)- and Cu(I)-binding features of the protozoan Tetrahymena pyriformis MT1 metallothionein. Biochim Biophys Acta 1784: 693-704.
4. Isart RN, Vasak M,. 2002. Advances in the structure and chemistry of metallothioneins. J Inorg Biochem 88: 388-396. (Pubitemid 34207852)
5. Klaassen CD, Liu J, Choudhuri S,. 1999. Metallothionein: An intracellular protein to protect against cadmium toxicity. Annu Rev Pharmacol Toxicol 39: 267-294. (Pubitemid 29222560)
6. Kyte J, Doolittle RF,. 1982. A simple method for displaying the hydropathic character of a protien. J Mol Biol 157: 105-132.
7. Laible M, Boonrod K,. 2009. Homemade site directed mutagenesis of wholec plasmids. JoVE. 27., DOI: 10.3791/1135.
8. Mejare M, Bulow L,. 2001. Metal-binding proteins and peptides in bioremediation and phytoremediation of heavy metals. Trends Biotechnol 19: 67-73. (Pubitemid 32112684)
9. Miles AT, Hawksworth GM, Beattie JH, Rodilla V,. 2000. Induction, regulation, degradation, and biological significance of mammalian metallothioneins. Crit Rev Biochem Mol Biol 35: 35-70. (Pubitemid 30137066)
10. Odawara F, Kurasaki M, Suzuki KM, Oikawa S, Emoto T, Yamasaki F, Arias AL, Kojima Y,. 1995. Expression of human metallothionein-2 in Escherichia coli: Cadmium tolerance of transformed cells. J Biochem 118: 1131-1137. (Pubitemid 26013799)
11. Raina S, Missiakas D,. 1997. Making and breaking disulfide bonds. Annu Rev Microbiol 51: 79-202.
12. Sambrook J, Russel DW,. 2001. Molecular cloning: A laboratory manual. New York: Cold Spring Harbor, Cold Spring Harbor Laboratory Press.
13. Sayers Z, Broullion P, Vorgais CS, Nolting H, Herms C, Koch MJH,. 1993. Cloning and expression of Saccharomyces cerevisiae copper-metallothionein gene in E. coli and characterization of the recombinant protein. Eur J Biochem 212: 521-528. (Pubitemid 23076121)
14. Shuja RN, Shakoori AR,. 2007. Identification, cloning and sequencing of a novel stress inducible metallothionein gene from locally isolated Tetrahymena tropicalis lahorensis. Gene 405: 19-26. (Pubitemid 350016658)
15. Shuja RN, Shakoori AR,. 2009. Identification and cloning of first cadmium metallothionein like gene from locally isolated ciliate, Paramecium sp. Mol Biol Rep 36: 549-560.
16. Zhang Y,. 2008. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics 9: 40-44.