13C solid-state NMR study of the 13C-labeled peptide, (E) 8GGLGGQGAG(A) 6GGAGQGGYGG as a model for the local structure of Nephila clavipes dragline silk (MaSp1) before and after spinning

Biopolymers

Volumn 97, Issue 6, 2012, Pages 347-354

  Yazawa, Koji ^a   Yamaguchi, Erika ^a   Knight, David ^b   Asakura, Tetsuo ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^b Oxford Biomaterials Ltd   (United Kingdom)

Author keywords

acid treatment; NMR; spider silk

Indexed keywords

ACID TREATED; ACID TREATMENTS; ACIDIC SOLUTIONS; C-TERMINUS; CONFORMATION CHANGE; CONFORMATIONAL CHANGE; DRAGLINE SILK; LOCAL STRUCTURE; N-TERMINALS; NEPHILA CLAVIPES; NEUTRAL PH; RANDOM COIL; RANDOM COIL CONFORMATION; SOLID STATE NMR; SPIDER DRAGLINE SILK; SPIDER SILKS; SPINNING PROCESS; WATERSOLUBLE;

NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SILK; SOLID STATE PHYSICS; SOLUTIONS;

PEPTIDES;

CARBON 13; GLUTAMYLGLUTAMYLGLUTAMYLGLUTAMYLGLUTAMYLGLUTAMYLGLUTAMYLGLUTAMYLGLYCYLGLYCYLLEUCYLGLUTAMINYLGLUTAMYLALANYLGLUTAMYLALANYLALANYLALANYLALANYLALANYLALANYLGLUTAMYLGLUTAMYLALANYLGLUTAMYLGLUTAMINYLGLUTAMYLGLUTAMYLLYSYLGLUTAMYLGLUTAMINE; MAJOR AMPULLATE SPIDROIN 1; PEPTIDE; SILK FIBROIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; ISOTOPE LABELING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN CONFORMATION; SILK; SPIDER; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; CARBON ISOTOPES; GLYCINE; HYDROGEN-ION CONCENTRATION; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SILK; SOLUTIONS; SPIDERS;

ARANEAE; NEPHILA CLAVIPES;

EID: 84858862889     PISSN: 00063525     EISSN: 10970282     Source Type: Journal    
DOI: 10.1002/bip.21718     Document Type: Article

References (27)

1. Vollrath, F.,;, Knight, D. P., Nature 2001, 410, 541-548.
2. Shao, Z.,;, Vollrath, F., Nature 2002, 418, 741-741.
3. Xu, M.,;, Lewis, R. V., Proc Natl Acad Sci U S A 1990, 87, 7120.
4. Hinman, M. B.,;, Lewis, R. V., J Biol Chem 1992, 267, 19320.
5. Peng, X.,;, Shao, Z.,;, Chen, X.,;, Knight, D. P.,;, Wu, P.,;, Vollrath, F., Biomacromolecules 2006, 6, 302-308.
6. Dicko, C.,;, Vollrath, F.,;, Kenney, J. M., Biomacromolecules 2004, 5, 704-710.
7. Chen, X.,;, Knight, D. P.,;, Vollrath, F., Biomacromolecules 2002, 3, 644-648.
8. Porter, D.,;, Vollrath, F., Soft Matter 2008, 4, 328-336.
9. Hronska, M.,;, Van Beek, J. D.,;, Williamson, P. T. F.,;, Vollrath, F.,;, Meier, B. H., Biomacromolecules 2004, 5, 834-839.
10. Simmons, A. H.,;, Michal, C. A.,;, Jelinski, L. W., Science 1996, 271, 84.
11. Liivak, O.,;, Flores, A.,;, Lewis, R.,;, Jelinski, L.W., Macromolecules 1997, 30, 7127-7130.
12. Michal, C. A.,;, Jelinski, L. W., J Biomol NMR 1998, 12, 231-241.
13. Kümmerlen, J.,;, Van Beek, D,;, Vollrath, F.,;, Meier, B. H., Macromolecules 1996, 29, 2920-2928.
14. Seidel, A.,;, Liivak, O.,;, Calve, S.,;, Adaska, J.,;, Ji, G.,;, Yang, Z.,;, Grubb, D.,;, Zax, D. B.,;, Jelinski, L. W., Macromolecules 2000, 33, 775-780.
15. Yang, M.,;, Nakazawa, Y.,;, Yamauchi, K.,;, Knight, D.,;, Asakura, T., Biomacromolecules 2005, 6, 3220-3226.
16. Asakura, T.,;, Yang, M.,;, Kawase, T.,;, Nakazawa, Y., Macromolecules 2005, 38, 3356-3363.
17. Colgin, M. A.,;, Lewis, R. V., Prot Sci 1998, 7, 667-672.
18. Bini, E.,;, Knight, D. P.,;, Kaplan, D. L., J Mol Biol 2004, 335, 27-40.
19. Rising, A.,;, Johansson, J.,;, Larson, G.,;, Bongcam-Rudloff, E.,;, Enstrom, W.,;, Hjalm, G., Insect Mol Biol 2007, 16, 551-561.
20. Saito, H.,;, Ando, I., In Annual Reports on NMR Spectroscopy, Vol. 22;, Webb, G. A., Ed.; Academic Press: London, 1989; pp 209-290.
21. Nakazawa, Y.,;, Asakura, T., J Am Chem Soc 2003, 125, 7230-7237.
22. Nakazawa, Y.,;, Bamba, M.,;, Nishio, S.,;, Asakura, T., Prot Sci 2003, 12, 666-671.
23. Hijirida, D. H.,;, Do, K. G.,;, Michal, C.,;, Wong, S.,;, Zax, D.,;, Jelinski, L. W., Biophys J 1996, 71, 3442-3447.
24. Kesvatera, T.,;, Jönsson, B.,;, Thulin, E.,;, Linse, S., Prot Struct Funct Bioinform 1999, 31, 106-115.
25. Asakura, T.,;, Okonogi, M.,;, Nakazawa, Y.,;, Yamauchi, K., J Am Chem Soc 2006, 128, 6231-6238.
26. Holland, G. P.,;, Jenkins, J. E.,;, Creager, M. S.,;, Lewis, R.V.,;, Yarger, J. L., Chem Commun 2008, 43, 5568-5570.
27. Jenkins, J. E.,;, Creager, M. S.,;, Lewis, R. V.,;, Holland, G. P.,;, Yarger, J. L., Biomacromolecules 2009, 11, 192-200.