Spider minor ampullate silk proteins contain new repetitive sequences and highly conserved non-silk-like 'spacer regions'

Protein Science

Volumn 7, Issue 3, 1998, Pages 667-672

  Colgin, Mark A ^a,b   Lewis, Randolph V ^a  

^a UNIVERSITY OF WYOMING   (United States)

^b Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

Minor ampullate; Protein; Sequence; Spider silk

Indexed keywords

ALANINE; ARGININE; GLUTAMINE; GLYCINE; PROTEIN; TYROSINE;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN DOMAIN; PROTEIN SYNTHESIS; SILK; SPIDER;

EID: 0031932874     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560070315     Document Type: Article

References (26)

1. Beckwitt R, Arcidiacono S. 1994. Sequence conservation in the C-terminal region of spider silk proteins (Spidroin) from Nephila clavipes (Tetragnathidae) and Araneus bicentenarius (Araneidaie). J Biol Chem 269:6661-6663.
2. Brown L, Trotter IF. 1960. X-ray studies of poly-L-alanine. Faraday Soc Trans 52:545-551.
3. Dong Z. 1992. [Doctoral dissertation]. University of Wyoming. pp 50-52.
4. Dong Z, Lewis RV, Middaugh CR. 1991. Molecular mechanism of spider silk elasticity. ArchBiochem Biophys 284:53-57.
5. Fossey SA et al. 1991. Conformational energy studies of β-sheets of model silk fibroin peptides. I. Sheets of poly(Ala-Gly) chains. Biopolymers 31:1529-1541.
6. Gillespie DB, Viney C, Yager P. 1994. Raman spectroscopic analysis of the secondary structure of spider silk fiber. In Kaplan D, Adams W, Farmer B, Viney C, eds. Silk polymers. Washington, DC: American Chemical Society. pp 155-167.
7. Gosline JM, DeMont ME, Denny ME. 1986. The structure and properties of spider silk. Endeavor 10:37-43.
8. Guerette PA, Ginzinger DG, Weber BHF, Gosline JM. 1996. Silk properties determined by gland specific expression of a spider fibroin gene family. Science 272:112-115.
9. Hayashi CY, Lewis RV. 1998. Evidence from flagelliform silk cDNA for the structural basis of elasticity and modular nature of spider silks. J Mol Biol 275:773-784.
10. Herrmann BG, Frischauf AM. 1987. Isolation of genomic DNA. Methods Enzymol 152:180-192.
11. Hinman MB, Lewis RV. 1992. Isolation of a clone encoding a second dragline silk fibroin. J Biol Chem 267:19320-19324.
12. Hinman MB, Stauffer SL, Lewis RV. 1994. Mechanical and chemical properties of certain spider silks. In: Kaplan D, Adams W, Farmer B, Viney C, eds. Silk polymers. Washington, DC: American Chemical Society. pp 222-233.
13. Lewis RV. 1992. Spider silk: The unraveling of a mystery. Acc Chem Res 25:392-398.
14. Lucas F. 1986. Spiders and their silk. Discovery 25:20-26.
15. Mertz LM, Rashtchian A. 1994. Nucleotide imbalance and polymerase chain reaction: Effects on DNA amplification and synthesis of high specific activity radiolabeled DNA probes. Anal Biochem 221:160-165.
16. Mita K, Ichimura S, James TC. 1994. Highly repetitive structure and its organization of the silk fibroin gene. J Mol Evol 38:583-592.
17. Parkhe AD, Seeley SK, Gardner K, Thompson L, Lewis RV. 1997. Structural studies of spider silk proteins in the fiber. J Molec Recog 10:1-6.
18. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: A laboratory manual, second edition. New York: Cold Spring Harbor Laboratory Press.
19. Simmons A, Michal C, Jelinski L. 1996. Molecular orientation and two-component nature of the crystalline fracaation of spider dragline silk. Science 271:84-87.
20. Stauffer SL, Coguill S, Lewis RV. 1994. Comparison of physical properties of three silks from Nephila clavipes and Araneus gemmoides. J Arachnol 22:5-11.
21. Tsuijimoto Y, Suzuki Y. 1979a. Structural analysis of the fibroin gene at the 5′ end and its surrounding region. Cell 16:425-431.
22. Tsuijimoto Y, Suzuki Y. 1979b. The DNA sequence of Bombyx mori fibroin gene including the 5′ flanking, mRNA coding, entire intervening and fibroin protein coding regions. Cell 18:591-600.
23. Vollrath F. 1992. Spider webs and silks. Scientific American 266:70-76.
24. Warwicker JO. 1960. Comparative studies of fibroins II. The crystal structure of various fibroins. Faraday Soc Trans 52:554-557.
25. Wieslander L. 1994. The balbiani ring multigene family: Coding repetitive sequences and evolution of a tissue-specific cell function. Prog Nucleic Acid Res Mol Biol 48:275-313.
26. Xu M, Lewis RV. 1990. Structure of a protein superfiber: Spider dragline silk. Proc Natl AcadSci USA 87:7120-7124.