Enhanced epifluorescence microscopy by uniform and intensity optimized illumination

Cytometry Part A

Volumn 81 A, Issue 4, 2012, Pages 278-280

  Herbert, Sébastien ^a   Henriques, Ricardo ^a,b  

^a CNRS   (France)

^b INSTITUTO DE MEDICINA MOLECULAR   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE MICROSCOPY; ILLUMINATION; LIGHT; METHODOLOGY; NOTE;

LIGHT; LIGHTING; MICROSCOPY, FLUORESCENCE;

EID: 84858792111     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.21177     Document Type: Note

References (12)

1. Murray JM, Appleton PL, Swedlow JR, Waters JC. Evaluating performance in three-dimensional fluorescence microscopy. J Microsc 2007; 228: 390-405.
2. Fiolka R, Belyaev Y, Ewers H, Stemmer A. Even illumination in total internal reflection fluorescence microscopy using laser light. Microsc Res Tech 2008; 71: 45-50.
3. Wolf DE, Samarasekera C, Swedlow JR. Quantitative analysis of digital microscope images. Methods Cell Biol 2007; 81: 365-396.
4. Waters JC. Accuracy and precision in quantitative fluorescence microscopy. J Cell Biol 2009; 185: 1135.
5. Salmon ED, Canman JC. Proper alignment and adjustment of the light microscope. Curr Protoc Cell Biol 2001; Chapter 4: Unit 4.1. http://dx.doi.org/10.1002/0471143030.cb0401s00.
6. Hoffnagle JA, Jefferson CM. Design and performance of a refractive optical system that converts a Gaussian to a flattop beam. Appl Opt 2000; 39: 5488-5499.
7. Wippermann F, Zeitner UD, Dannberg P, Braüer A, Sinzinger S. Beam homogenizers based on chirped microlens arrays. Opt Express 2007; 15: 6218-6231.
8. Nemoto K, Fujii T, Goto N, Takino H, Kobayashi T, Shibata N, Yamamura K, Mori Y. Laser beam intensity profile transformation with a fabricated mirror. Appl Opt 1997; 36: 551-557.
9. Chang SP, Kuo JM, Lee YP, Lu CM, Ling KJ. Transformation of Gaussian to coherent uniform beams by inverse-Gaussian transmittive filters. Appl Opt 1998; 37: 747-752.
10. Sales TRM. Structured microlens arrays for beam shaping. Opt Eng 2003; 42: 3084.
11. Zucker RM, Hunter ES, Rogers JM. Confocal laser scanning microscopy of morphology and apoptosis in organogenesis-stage mouse embryos. Methods Mol Biol 2000; 135: 191-202.
12. Caarls W, Rieger B, De Vries AH, Arndt-Jovin DJ, Jovin TM. Minimizing light exposure with the programmable array microscope. J Microsc 2011; 241: 101-110.