Even illumination in total internal reflection fluorescence microscopy using laser light

Microscopy Research and Technique

Volumn 71, Issue 1, 2008, Pages 45-50

  Fiolka, R ^a   Belyaev, Y ^a,b   Ewers, H ^a   Stemmer, A ^a  

^a ETH ZURICH   (Switzerland)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

Coherence; Evanescent fields; Illumination; Intereference fringes; Total internal reflection fluorescence (TIRF) microscopy

Indexed keywords

COHERENT SCATTERING; FLUORESCENCE; FLUORESCENCE MICROSCOPY; LASER BEAMS; REFRACTIVE INDEX;

DESIGN AND CONSTRUCTION; EPI-ILLUMINATION; IMAGE-ANALYSIS; INTEREFERENCE FRINGE; LASER LIGHTS; NON-UNIFORM ILLUMINATION; SOURCE OF LIGHT; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY; TOTAL INTERNAL REFLECTIONS; WIDE FIELD IMAGING;

EVANESCENT FIELDS;

ACTIN;

ARTICLE; CONTROLLED STUDY; CYTOSKELETON; ENDOCYTOSIS; EPIFLUORESCENCE MICROSCOPE; EXOCYTOSIS; FLUORESCENCE MICROSCOPY; HELA CELL; HUMAN; HUMAN CELL; ILLUMINATION; IMAGE ANALYSIS; LASER; LIGHT SCATTERING; PRIORITY JOURNAL; REFRACTION INDEX;

EID: 38149078767     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20527     Document Type: Article

References (16)

1. Aravanis AM, Pyle JL, Tsien RW. 2003. Single synaptic vesicles fusing transiently and successively without loss of identity. Nature 423:643-647.
2. Axelrod D. 2001. Total internal reflection fluorescence microscopy in cell biology. Traffic 2:764-774.
3. Axelrod D. 2003. Total internal reflection fluorescence microscopy in cell biology. Biophotonics, Part B. San Diego: Academic Press. pp. 1-33.
4. Chew HW, Wang D-S, Kerker M. 1979. Elastic scattering of evanescent electromagnetic waves. Appl Opt 18:2679-2687.
5. Ellis GW. 1988. An annular scan phase-contrast scanned aperture microscope (Aspsam). Cell Motil Cytoskeleton 10:342.
6. Mattheyses AL, Axelrod D. 2005. Effective elimination of laser interference fringing in fluorescence microscopy by spinning azimuthal incidence angle. Biophys J 88:341A-342A.
7. Mattheyses AL, Axelrod D. 2006. Direct measurement of the evanescent field profile produced by objective-based total internal reflection fluorescence. J Biomed Opt 11:014006.
8. Oheim M, Schapper F. 2005. Non-linear evanescent-field imaging. J Phys D: Appl Phys 38:185-197.
9. Pelkmans L, Kartenbeck J, Helenius A. 2001. Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER. Nat Cell Biol 3:473-483.
10. Pelkmans L, Zerial M. 2005. Kinase-regulated quantal assemblies and kiss-and-run recycling of caveolae. Nature 436:128-133.
11. Rohrbach A. 2000. Observing secretory granules with a multiangle evanescent wave microscope. Biophys J 78:2641-2654.
12. Schneckenburger H. 2005. Total internal reflection fluorescence microscopy: Technical innovations and novel applications. Curr Opin Biotechnol 16:13-18.
13. Smith AE, Ewers H, Sbalzarini I, Koumoutsakos P, Helenius A. 2004. Alternate endocytic pathways revealed by virus entry into animal cells. Mol Biol Cell 15:195A.
14. Steyer JA, Almers W. 1999. Tracking single secretory granules in live chromaffin cells by evanescent-field fluorescence microscopy. Biophys J 76:2262-2271.
15. Stock K, Sailer R, Strauss WSL, Lyttek M, Steiner R, Schneckenburger H. 2003. Variable-angle total internal reflection fluorescence microscopy (VA-TIRFM): Realization and application of a compact illumination device. J Microsc (Oxford) 211:19-29.
16. Toomre D, Steyer JA, Keller P, Aimers W, Simons K 2000. Fusion of constitutive membrane traffic with the cell surface observed by evanescent wave microscopy. J Cell Biol 149:33-40.