Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

Journal of Proteomics

Volumn 75, Issue 7, 2012, Pages 2236-2251

  Stadler, Charlotte ^a   Hjelmare, Martin ^a   Neumann, Beate ^b   Jonasson, Kalle ^c   Pepperkok, Rainer ^b   Uhlén, Mathias ^a,c   Lundberg, Emma ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

Antibody validation; Immunofluorescence; Quantitative image analysis; SiRNA; Subcellular location

Indexed keywords

ANTIBODY; SMALL INTERFERING RNA;

ACTIN FILAMENT; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CELL COMPARTMENTALIZATION; CELL MEMBRANE; CELL NUCLEUS; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CYTOPLASM; DOWN REGULATION; ENDOPLASMIC RETICULUM; FUNCTIONAL PROTEOMICS; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IMMUNOFLUORESCENCE; MITOCHONDRION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; QUANTITATIVE ANALYSIS; RNA INTERFERENCE; SIGNAL TRANSDUCTION; VALIDATION STUDY; WESTERN BLOTTING;

ANTIBODIES; CELL LINE, TUMOR; HUMANS; MICROSCOPY, CONFOCAL; PROTEOME; RNA, SMALL INTERFERING;

EID: 84858748188     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2012.01.030     Document Type: Article

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