Non-viral transfection of goat germline stem cells by nucleofection results in production of transgenic sperm after germ cell transplantation

Molecular Reproduction and Development

Volumn 79, Issue 4, 2012, Pages 255-261

  Zeng, W ^a,e   Tang, L ^b   Bondareva, A ^b   Luo, J ^a   Megee, S O ^a   Modelski, M ^a   Blash, S ^c   Melican, D T ^c   Destrempes, M M ^c   Overton, S A ^c   Gavin, W G ^c   Ayres, S ^d   Echelard, Y ^c   Dobrinski, I ^a,b  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF CALGARY   (Canada)

^c GTC Biotherapeutics Inc   (United States)

^d TUFTS UNIVERSITY   (United States)

^e NORTHWEST A F UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOVIRUS VECTOR; BETA CASEIN; BETA GLOBIN; GROWTH HORMONE;

ADENOVIRUS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CANNULATION; CELL COUNT; CELL NUCLEUS TRANSPLANTATION; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; DNA EXTRACTION; DONOR; EJACULATION; ELECTROPORATION; GAMETE; GENETIC TRANSFECTION; GENOME ANALYSIS; GERM CELL; GERM LINE GENE THERAPY; GERMLINE STEM CELL; GOAT; HAPLOIDY; IMMUNOFLUORESCENCE; IN VITRO STUDY; MALE; MICROINJECTION; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; NUCLEOFECTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROGENY; PROMOTER REGION; RADIATION DOSE FRACTIONATION; RECIPIENT; SEDIMENTATION; SEMEN ANALYSIS; SPERMATOGONIUM; STEM CELL TRANSPLANTATION; TESTIS; TRANSGENE; TRANSGENICS; ULTRASOUND;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BETA-GLOBINS; CASEINS; CHICKENS; FEMALE; GENOTYPE; GERM CELLS; GOATS; HUMAN GROWTH HORMONE; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; PROMOTER REGIONS, GENETIC; SPERMATOZOA; STEM CELL TRANSPLANTATION; STEM CELLS; TESTIS; TRANSFECTION; TRANSGENES;

ANIMALIA; CAPRA HIRCUS;

EID: 84858702960     PISSN: 1040452X     EISSN: 10982795     Source Type: Journal    
DOI: 10.1002/mrd.22014     Document Type: Article

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