Mutations in the β subunit of RNA polymerase alter intrinsic cephalosporin resistance in enterococci

Antimicrobial Agents and Chemotherapy

Volumn 56, Issue 4, 2012, Pages 2022-2027

  Kristich, Christopher J ^a   Little, Jaime L ^a  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPICILLIN; BACITRACIN; CEFADROXIL; CEFTAZIDIME; CEFTRIAXONE; CEPHALOSPORIN; CHLORAMPHENICOL; CYCLOSERINE; KANAMYCIN; NORFLOXACIN; RIFAMPICIN; RNA POLYMERASE BETA SUBUNIT; VANCOMYCIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; BINDING SITE; BIOSYNTHESIS; CELL GROWTH; CELL STRESS; CELL WALL; CONTROLLED STUDY; ENTEROCOCCUS; ENTEROCOCCUS FAECALIS; GENE FREQUENCY; GENETIC LINKAGE; MINIMUM INHIBITORY CONCENTRATION; MISSENSE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING;

ANTITUBERCULAR AGENTS; CEPHALOSPORIN RESISTANCE; CULTURE MEDIA; DNA-DIRECTED RNA POLYMERASES; ENTEROCOCCUS; GENETIC LINKAGE; MICROBIAL SENSITIVITY TESTS; MUTATION; PLASMIDS; RIFAMPIN;

EID: 84858667570     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.06077-11     Document Type: Article

References (40)

1. Aarestrup FM, Butaye P, Witte W. 2002. Nonhuman reservoirs of enterococci, p 55-99. In Gilmore MS, et al (ed), The enterococci: pathogenesis, molecular biology, and antibiotic resistance. American Society for Microbiology Press, Washington, DC.
2. Arbeloa A, et al. 2004. Role of class A penicillin-binding proteins in PBP5-mediated β-lactam resistance in Enterococcus faecalis. J. Bacteriol. 186:1221-1228. (Pubitemid 38248710)
3. Aubry-Damon H, Soussy CJ, Courvalin P. 1998. Characterization of mutations in the rpoB gene that confer rifampin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 42:2590-2594. (Pubitemid 28462898)
4. Canepari P, Lleo MM, Cornaglia G, Fontana R, Satta G. 1986. In Streptococcus faecium penicillin-binding protein 5 alone is sufficient for growth at sub-maximal but not at maximal rate. J. Gen. Microbiol. 132:625-631. (Pubitemid 16064044)
5. Clewell DB, et al. 1982. Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and studies relating to transposition of Tn917. J. Bacteriol. 152:1220-1230. (Pubitemid 13190428)
6. Comenge Y, et al. 2003. The CroRS two-component regulatory system is required for intrinsic β-lactam resistance in Enterococcus faecalis. J. Bacteriol. 185:7184-7192. (Pubitemid 37509835)
7. Cui L, et al. 2010. An RpoB mutation confers dual heteroresistance to daptomycin and vancomycin in Staphylococcus aureus. Antimicrob. Agents Chemother. 54:5222-5233.
8. Donskey CJ, et al. 2000. Effect of antibiotic therapy on the density of vancomycin-resistant enterococci in the stool of colonized patients. N. Engl. J. Med. 343:1925-1932. (Pubitemid 32064786)
9. Dunny GM, Brown BL, Clewell DB. 1978. Induced cell aggregation and mating in Streptococcus faecalis: evidence for a bacterial sex pheromone. Proc. Natl. Acad. Sci. U. S. A. 75:3479-3483. (Pubitemid 8395096)
10. Floss HG, Yu TW. 2005. Rifamycin-mode of action, resistance, and biosynthesis. Chem. Rev. 105:621-632.
11. Gold OG, Jordan HV, van Houte J. 1975. The prevalence of enterococci in the human mouth and their pathogenicity in animal models. Arch. Oral Biol. 20:473-477.
12. Hancock LE, Perego M. 2004. Systematic inactivation and phenotypic characterization of two-component signal transduction systems of Enterococcus faecalis V583. J. Bacteriol. 186:7951-7958. (Pubitemid 39552506)
13. Hegstad K, Mikalsen T, Coque TM, Werner G, Sundsfjord A. 2010. Mobile genetic elements and their contribution to the emergence of antimicrobial resistant Enterococcus faecalis and Enterococcus faecium. Clin. Microbiol. Infect. 16:541-554.
14. Hidron AI, et al. 2008. NHSN annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2006-2007. Infect. Control Hosp. Epidemiol. 29:996-1011.
15. Inaoka T, Takahashi K, Yada H, Yoshida M, Ochi K. 2004. RNA polymerase mutation activates the production of a dormant antibiotic 3,3′- neotrehalosadiamine via an autoinduction mechanism in Bacillus subtilis. J. Biol. Chem. 279:3885-3892. (Pubitemid 38140634)
16. Ingham CJ, Furneaux PA. 2000. Mutations in the beta subunit of the Bacillus subtilis RNA polymerase that confer both rifampicin resistance and hypersensitivity to NusG. Microbiology 146:3041-3049. (Pubitemid 32007660)
17. Jin DJ, Gross CA. 1988. Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance. J. Mol. Biol. 202:45-58.
18. Kristich CJ, Chandler JR, Dunny GM. 2007. Development of a hostgenotype-independent counterselectable marker and a high-frequency conjugative delivery system and their use in genetic analysis of Enterococcus faecalis. Plasmid 57:131-144.
19. Kristich CJ, Little JL, Hall CL, Hoff JS. 2011. Reciprocal regulation of cephalosporin resistance in Enterococcus faecalis. mBio 2(6):e00199-11. doi:10.1128/mBio.00199-11.
20. Kristich CJ, Manias DA, Dunny GM. 2005. Development of a method for markerless genetic exchange in Enterococcus faecalis and its use in construction of a srtA mutant. Appl. Environ. Microbiol. 71:5837-5849. (Pubitemid 41456875)
21. Kristich CJ, Wells CL, Dunny GM. 2007. A eukaryotic-type Ser/Thr kinase in Enterococcus faecalis mediates antimicrobial resistance and intestinal persistence. Proc. Natl. Acad. Sci. U. S. A. 104:3508-3513. (Pubitemid 46364189)
22. Le Breton Y, Muller C, Auffray Y, Rince A. 2007. New insights into the Enterococcus faecalis CroRS two-component system obtained using a differential-display random arbitrarily primed PCR approach. Appl. Environ. Microbiol. 73:3738-3741. (Pubitemid 46871467)
23. Maekawa S, Yoshioka M, Kumamoto Y. 1992. Proposal of a new scheme for the serological typing of Enterococcus faecalis strains. Microbiol. Immunol. 36:671-681.
24. Maughan H, Galeano B, Nicholson WL. 2004. Novel rpoB mutations conferring rifampin resistance on Bacillus subtilis: global effects on growth, competence, sporulation, and germination. J. Bacteriol. 186: 2481-2486. (Pubitemid 38451852)
25. McBride SM, Fischetti VA, Leblanc DJ, Moellering RC, Jr, Gilmore MS. 2007. Genetic diversity among Enterococcus faecalis. PLoS One 2:e582.
26. Muller C, et al. 2006. The response regulator CroR modulates expression of the secreted stress-induced SalB protein in Enterococcus faecalis. J. Bacteriol. 188:2636-2645.
27. Murray BE. 1990. The life and times of the Enterococcus. Clin. Microbiol. Rev. 3:46-65. (Pubitemid 20078774)
28. Nicholson WL, Maughan H. 2002. The spectrum of spontaneous rifampin resistance mutations in the rpoB gene of Bacillus subtilis 168 spores differs from that of vegetative cells and resembles that of Mycobacterium tuberculosis. J. Bacteriol. 184:4936-4940.
29. Ozawa Y, De Boever EH, Clewell DB. 2005. Enterococcus faecalis sex pheromone plasmid pAM373: analyses of TraA and evidence for its interaction with RpoB. Plasmid 54:57-69. (Pubitemid 40723639)
30. Palmer KL, et al. 2010. High-quality draft genome sequences of 28 Enterococcus sp. isolates. J. Bacteriol. 192:2469-2470.
31. Reference deleted.
32. Perkins AE, Nicholson WL. 2008. Uncovering new metabolic capabilities of Bacillus subtilis using phenotype profiling of rifampin-resistant rpoB mutants. J. Bacteriol. 190:807-814. (Pubitemid 351170923)
33. Signoretto C, Boaretti M, Canepari P. 1994. Cloning, sequencing and expression in Escherichia coli of the low-affinity penicillin binding protein of Enterococcus faecalis. FEMS Microbiol. Lett. 123:99-106. (Pubitemid 24312570)
34. Tannock GW, Cook G. 2002. Enterococci as members of the intestinal microflora of humans, p 101-132. In Gilmore MS, et al (ed), The enterococci: pathogenesis, molecular biology, and antibiotic resistance. American Society for Microbiology Press, Washington, DC.
35. Tomasz A. 1979. The mechanism of the irreversible antimicrobial effects of penicillins: how the beta-lactam antibiotics kill and lyse bacteria. Annu. Rev. Microbiol. 33:113-137.
36. Vesic D, Kristich CJ. 30 January 2012. MurAA is required for intrinsic cephalosporin resistance of Enterococcus faecalis. Antimicrob. Agents Chemother. doi:10.1128/AAC.05984-11.
37. Vogler AJ, et al. 2002. Molecular analysis of rifampin resistance in Bacillus anthracis and Bacillus cereus. Antimicrob. Agents Chemother. 46:511-513. (Pubitemid 34087202)
38. Wehrli W, Knusel F, Schmid K, Staehelin M. 1968. Interaction of rifamycin with bacterial RNA polymerase. Proc. Natl. Acad. Sci. U. S. A. 61:667-673.
39. Zapun A, Contreras-Martel C, Vernet T. 2008. Penicillin-binding proteins and beta-lactam resistance. FEMS Microbiol. Rev. 32:361-385.
40. Zhou YN, Jin DJ. 1998. The rpoB mutants destabilizing initiation complexes at stringently controlled promoters behave like "stringent" RNA polymerases in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 95:2908-2913. (Pubitemid 128617610)