Multiplex IP-FCM (immunoprecipitation-flow cytometry): Principles and guidelines for assessing physiologic protein-protein interactions in multiprotein complexes

Methods

Volumn 56, Issue 2, 2012, Pages 154-160

  Bida, Anya T ^a   Gil, Diana ^a   Schrum, Adam G ^a  

^a MAYO GRADUATE SCHOOL   (United States)

Author keywords

Flow cytometry; IP FCM; Multiplex; Multiprotein complex; Protein protein interaction

Indexed keywords

MULTIPROTEIN COMPLEX;

ARTICLE; CONTROLLED STUDY; EXPERIMENTAL STUDY; FLOW CYTOMETRY; IMMUNOPRECIPITATION; INFORMATION PROCESSING; MULTIPLEX IMMUNOPRECIPITATION FLOW CYTOMETRY; PRACTICE GUIDELINE; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN PROTEIN INTERACTION; SINGLEPLEX IMMUNOPRECIPITATION FLOW CYTOMETRY; VALIDITY;

ANIMALS; ANTIBODIES; ANTIBODY SPECIFICITY; CELL LINE, TUMOR; FLOW CYTOMETRY; FLUORESCENT DYES; IMMUNOPRECIPITATION; MICE; MICROSPHERES; MULTIPROTEIN COMPLEXES; PROTEIN INTERACTION MAPPING; PROTEIN STABILITY; RECEPTORS, ANTIGEN, T-CELL; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; TITRIMETRY;

EID: 84858451531     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2011.09.005     Document Type: Article

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