Orientation selective DEER measurements on vinculin tail at X-band frequencies reveal spin label orientations

Journal of Magnetic Resonance

Volumn 216, Issue , 2012, Pages 53-61

  Abé, Christoph ^a   Klose, Daniel ^a   Dietrich, Franziska ^b,e   Ziegler, Wolfgang H ^b,c   Polyhach, Yevhen ^d   Jeschke, Gunnar ^d   Steinhoff, Heinz Jürgen ^a  

^a UNIVERSITY OF OSNABRÜCK   (Germany)

^b UNIVERSITY HOSPITAL LEIPZIG   (Germany)

^c HANNOVER MEDICAL SCHOOL   (Germany)

^d ETH ZURICH   (Switzerland)

^e UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Orientation selective DEER; PELDOR; Site directed spin labeling

Indexed keywords

ASSIGNMENT OF; CYTOSKELETAL PROTEINS; DIHEDRAL RESTRAINTS; DOUBLE ELECTRON-ELECTRON RESONANCE; EQUIVALENT PARAMETERS; GLOBAL FITTING; GRID SEARCH; MOLECULAR AXES; MOLECULAR FRAME; MUTUAL ORIENTATION; NITROXIDES; PARAMETER SPACES; PARAMETERIZED; PELDOR; PRIMARY DATA; PROTEIN MOLECULES; RELATIVE ORIENTATION; SELECTIVE EXCITATIONS; SITE-DIRECTED SPIN LABELING; SPIN HAMILTONIAN; SPIN LABEL; SPIN-SPIN; STRONG ORIENTATION; VINCULIN; X-BAND FREQUENCIES;

DIHEDRAL ANGLE; EXPERIMENTS;

MOLECULAR ORIENTATION;

COMPLEMENTARY DNA; NITROGEN OXIDE; VINCULIN;

ALGORITHM; ARTICLE; BIOSYNTHESIS; CHEMISTRY; COMPUTER PROGRAM; ELECTRON SPIN RESONANCE; FOURIER ANALYSIS; GENETICS; METHODOLOGY; NORMAL DISTRIBUTION; SOLUTION AND SOLUBILITY; SPIN LABELING;

ALGORITHMS; DNA, COMPLEMENTARY; ELECTRON SPIN RESONANCE SPECTROSCOPY; FOURIER ANALYSIS; NITROGEN OXIDES; NORMAL DISTRIBUTION; SOFTWARE; SOLUTIONS; SPIN LABELS; VINCULIN;

AXIS (DEER);

EID: 84858333329     PISSN: 10907807     EISSN: 10960856     Source Type: Journal    
DOI: 10.1016/j.jmr.2011.12.024     Document Type: Article

References (21)

1. G. Jeschke, and Y. Polyhach Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance Phys. Chem. Chem. Phys. 9 2007 1895 1910
2. M. Pannier, S. Veit, A. Godt, G. Jeschke, and H.W. Spiess Dead-time free measurement of dipole-dipole interactions between electron spins J. Magn. Res. 142 2000 331 340
3. R.G. Larsen, and D.J. Singel Double electron-electron resonance spin-echo modulation - spectroscopic measurement of electron-spin pair separations in orientationally disordered solids J. Chem. Phys. 98 1993 5134 5146
4. V.P. Denysenkov, T.F. Prisner, J. Stubbe, and M. Bennati High-field pulsed electron-electron double resonance spectroscopy to determine the orientation of the tyrosyl radicals in ribonucleotide reductase Proc. Natl. Acad. Sci. USA 103 2006 13386 13390 (Pubitemid 44380117)
5. Y. Polyhach, A. Godt, C. Bauer, and G. Jeschke Spin pair geometry revealed by high-field DEER in the presence of conformational distributions J. Magn. Res. 185 2007 118 129 (Pubitemid 46501770)
6. A. Savitsky, A. Dubinskii, H. Zimmermann, W. Lubitz, and K. Moebius High-field dipolar epr spectroscopy of nitroxide biradicals for determining three-dimensional structures of biomacromolecules in disordered solids The J. Phys. Chem. B 115 2011 11950 11963
7. A. Potapov, H. Yagi, T. Huber, S. Jergic, N.E. Dixon, G. Otting, and D. Goldfarb Nanometer-scale distance measurements in proteins using Gd3+ spin labeling J. Am. Chem. Soc. 132 2010 9040 9048
8. D. Margraf, B.E. Bode, A. Marko, O. Schiemann, and T.F. Prisner Conformational flexibility of nitroxide biradicals determined by X-band PELDOR experiments Mol. Phys. 105 2007 2153 2160 (Pubitemid 351426631)
9. A. Marko, D. Margraf, H. Yu, Y. Mu, G. Stock, and T.F. Prisner Molecular orientation studies by pulsed electron-electron double resonance experiments J. Chem. Phys. 130 2009 064102
10. A. Marko, D. Margraf, P. Cekan, S.T. Sigurdsson, O. Schiemann, and T.F. Prisner Analytical method to determine the orientation of rigid spin labels in DNA Phys. Rev. E 81 2010 021911
11. A. Godt, M. Schulte, H. Zimmermann, and G. Jeschke How flexible are poly(para-phenyleneethynylene)s? Angew. Chem. Int. Ed. 45 2006 7560 7564
12. G. Jeschke, M. Sajid, M. Schulte, N. Ramezanian, A. Volkov, H. Zimmermann, and A. Godt Flexibility of shape-persistent molecular building blocks composed of p-phenylene and ethynylene units J. Am. Chem. Soc. 132 2010 10107 10117
13. A. Savitsky, AA. Dubinskii, M. Flores, W. Lubitz, and K. Möbius Orientation-resolving pulsed electron dipolar high-field EPR spectroscopy on disordered solids: I Structure of spin-correlated radical pairs in bacterial photosynthetic reaction centers J. Phys. Chem. B. 111 2007 6245 6262 (Pubitemid 46966232)
14. J.E. Lovett, A.M. Bowen, C.R. Timmel, M.W. Jones, J.R. Dilworth, D. Caprotti, S.G. Bell, L.L. Wong, and J. Harmer Structural information from orientationally selective DEER spectroscopy Phys. Chem. Chem. Phys. 11 2009 6840 6848
15. B. Endeward, JA. Butterwick, R. MacKinnon, and TF. Prisner Pulsed electron-electron double-resonance determination of spin-label distances and orientations on the tetrameric potassium ion channel KcsA J. Am. Chem. Soc. 131 2009 15246 15250
16. I. Chandrasekar, T.E.B. Stradal, M.R. Holt, F. Entschladen, B.M. Jockusch, and W.H. Ziegler Vinculin acts as a sensor in lipid regulation of adhesion-site turnover J. Cell. Sci. 118 2005 1461 1472 (Pubitemid 40585126)
17. W.H. Ziegler, U. Tigges, A. Zieseniss, and B.M. Jockusch A lipid-regulated docking site on vinculin for protein kinase C J. Biol. Chem. 277 2002 7396 7404 (Pubitemid 34953136)
18. C. Abe, F. Dietrich, P. Gajula, M. Benz, K.P. Vogel, M. van Gastel, S. Illenberger, W.H. Ziegler, and H.-J. Steinhoff Monomeric and dimeric conformation of the vinculin tail five-helix bundle in solution studied by EPR spectroscopy Biophys. J. 101 2011 1772 1780
19. G. Jeschke, V. Chechik, P. Ionita, A. Godt, H. Zimmermann, J.E. Banham, C.R. Timmel, D. Hilger, and H. Jung DeerAnalysis2006 - a comprehensive software package for analyzing pulsed ELDOR data Appl. Magn. Reson. 30 2006 473 498
20. S. Stoll, A. Schweiger, and EasySpin a comprehensive software package for spectral simulation and analysis in EPR J. Magn. Res. 178 2006 42 55 (Pubitemid 41774010)
21. P. Lueders, G. Jeschke, and M. Yulikov Double electron-electron resonance measured between Gd3+ ions and nitroxide radicals J. Phys. Chem. Lett. 2 2011 604 609