A novel fluorometric enzyme analysis method for Hunter syndrome using dried blood spots

Molecular Genetics and Metabolism

Volumn 105, Issue 3, 2012, Pages 519-521

  Tolun, Adviye A ^a   Graham, Carrie ^b   Shi, Qun ^a   Sista, Ramakrishna S ^b   Wang, Tong ^b   Eckhardt, Allen E ^b   Pamula, Vamsee K ^b   Millington, David S ^a   Bali, Deeksha S ^a  

^a DUKE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Advanced Liquid Logic   (United States)

Author keywords

Digital microfluidics; Dried blood spot; Enzyme assay; Hunter syndrome; Iduronate 2 sulfatase; Microplate

Indexed keywords

ARTICLE; CONTROLLED STUDY; DIGITAL MICROFLUIDIC; DRIED BLOOD SPOT TESTING; ENZYME ASSAY; FLUOROMETRY; HUMAN; HUNTER SYNDROME; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MICROFLUIDIC ANALYSIS; PRIORITY JOURNAL;

DRIED BLOOD SPOT TESTING; ENZYME ASSAYS; FLUOROMETRY; HUMANS; IDURONATE SULFATASE; MICROFLUIDIC ANALYTICAL TECHNIQUES; MUCOPOLYSACCHARIDOSIS II;

EID: 84858294104     PISSN: 10967192     EISSN: 10967206     Source Type: Journal    
DOI: 10.1016/j.ymgme.2011.12.011     Document Type: Article

References (6)

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5. Sista R., Eckhardt A.E., Wang T., Sellos-Moura M., Pamula V.K. Rapid, single-step assay for Hunter syndrome in dried blood spots using digital microfluidics. Clin. Chim. Acta 2011, 412:1895-1897.
6. Voznyi Y.V., Keulemans J.L., van Diggelen O.P. A fluorimetric enzyme assay for the diagnosis of MPS II (Hunter disease). J. Inherit. Metab. Dis. 2001, 24:675-680.