Identification and application of NEDD8 E1 inhibitors

Methods in Molecular Biology

Volumn 832, Issue , 2012, Pages 577-588

  Bruzzese, Frank J ^a   Milhollen, Michael A ^a   Gavin, James M ^a   Josephine, Helen R ^a   Brownell, James E ^a  

^a MILLENNIUM PHARMACEUTICALS INC   (United States)

Author keywords

APPBP1 UBA3; NEDD8; NEDD8 activating enzyme; Pyrophosphate exchange; UBC12

Indexed keywords

CULLIN; NEDD8 PROTEIN, HUMAN; UBIQUITIN; UBIQUITIN PROTEIN LIGASE;

ARTICLE; DRUG ANTAGONISM; ENZYME ACTIVATION; HUMAN; METABOLISM; TUMOR CELL LINE;

CELL LINE, TUMOR; CULLIN PROTEINS; ENZYME ACTIVATION; HUMANS; UBIQUITIN-ACTIVATING ENZYMES; UBIQUITINS;

EID: 84858200130     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-474-2_40     Document Type: Article

References (4)

1. Schulman BA, Harper JW (2009) Ubiquitinlike protein activation by E1 enzymes; the apex for downstream signalling pathways. Nat Rev Mol Cell Biol 10 :319-331.
2. Soucy TA, Smith PG, Milhollen MA et al (2009) An Inhibitor of NEDD8-activating enzyme as a new approach to treat cancer. Nature 458 :732-737.
3. Bohnsack RN, Haas AL (2003) Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. J Biol Chem 278 :26823-26830.
4. Bruzzese FJ, Tsu CA, Ma J et al (2009) Development of a charcoal paper adenosine triphosphate: Pyrophosphatre exchange assay: kinetic characterization of NEDD8 activating enzyme. Anal Biochem 394:24-29.