Development of a charcoal paper adenosine triphosphate:pyrophosphate exchange assay: Kinetic characterization of NEDD8 activating enzyme

Analytical Biochemistry

Volumn 394, Issue 1, 2009, Pages 24-29

  Bruzzese, Frank J ^a   Tsu, Christopher A ^a   Ma, Jingya ^a   Loke, Huay Keng ^a   Wu, Dongyun ^a   Li, Zhi ^a   Tayber, Olga ^a   Dick, Lawrence R ^a  

^a MILLENNIUM PHARMACEUTICALS INC   (United States)

Author keywords

APP BP 1 UBA3; ATP; Charcoal paper; E1; Inorganic pyrophosphate; NEDD8 activating enzyme; Ubiquitin like modifier

Indexed keywords

ADENOSINETRIPHOSPHATE; CHEMICAL ACTIVATION; NUCLEOTIDES; PHOSPHATES;

ADENYLATE; C TERMINUS; INITIAL VELOCITIES; INORGANIC PYROPHOSPHATE; KINETIC CHARACTERIZATION; UBIQUITIN; UBIQUITIN-LIKE;

ENZYMES;

ACTIVATED CARBON; ADENOSINE TRIPHOSPHATE; NEDD8 PROTEIN; NUCLEOTIDE; PHOSPHORUS 32; PYROPHOSPHATE; UBIQUITIN; UBIQUITIN PROTEIN LIGASE;

ACCURACY; ADENYLATION; ARTICLE; ASSAY; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONJUGATION; CONTROLLED STUDY; PRIORITY JOURNAL; REPRODUCIBILITY; SCINTILLATION; SENSITIVITY AND SPECIFICITY;

EID: 69249217687     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.07.011     Document Type: Article

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