Galleria mellonella as a model for fungal pathogenicity testing

Methods in Molecular Biology

Volumn 845, Issue , 2012, Pages 469-485

  Fallon, John ^a   Kelly, Judy ^a   Kavanagh, Kevin ^a  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

Author keywords

Aspergillus; Candida; Fungi; Galleria; Haemocytes; Microbial virulence; Mini host

Indexed keywords

ANTIFUNGAL AGENT;

ANIMAL; ARTICLE; CANDIDA ALBICANS; DISEASE MODEL; DRUG EFFECT; HOST PATHOGEN INTERACTION; IMMUNOLOGY; LARVA; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MOTH; PATHOGENICITY;

ANIMALS; ANTIFUNGAL AGENTS; CANDIDA ALBICANS; DISEASE MODELS, ANIMAL; HOST-PATHOGEN INTERACTIONS; LARVA; MICROBIAL SENSITIVITY TESTS; MOTHS;

ASPERGILLUS; CANDIDA; FUNGI; GALLERIA; GALLERIA MELLONELLA; HEXAPODA; MAMMALIA;

EID: 84858139967     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-539-8_33     Document Type: Chapter

References (17)

1. Kavanagh K. and Reeves E.P. (2004). Exploiting the potential of insects for in vivo pathogenicity testing of microbial pathogens. FEMS Microbiology Reviews. 28: 101–112.
2. Cotter G., Doyle S. and Kavanagh K. (2000). Development of an insect model for the in vivo pathogenicity testing of yeasts. FEMS Immunol. & Med. Microbiol. 27: 163–169.
3. Reeves E.P., Messina C.G.M., Doyle S. and Kavanagh K. (2004). Correlation of gliotoxin production and virulence of Aspergillus fumigatus in Galleria mellonella. Mycopathologia 158: 73–79
4. Brennan M., Thomas D.Y., Whiteway M.,, and Kavanagh K. (2002). Correlation between virulence of Candida albicans mutants in mice and Galleria mellonella larvae. FEMS Immunol. & Med. Microbiol 34: 153–157.
5. Mylonakis E., Moreno R., El Khoury J.B., Idnurm A., Heitman J., Calderwood S.B., Ausubel F.M. and Diener A. (2005). Galleria mellonella as a model system to study Cryptococcus neoformans pathogenesis. Infect. Immun. 73: 3842–3850.
6. Lionakis M.S., Lewis R.E., May G.S., Wiederhold N.P., Albert N.D., Halder G., and Kontoyiannis D.P. (2005). Toll-deficient Drosophila flies as a fast, high-throughput model for the study of antifungal drug efficacy against invasive aspergillosis and Aspergillus virulence. J Infect Dis. 191: 1188–95.
7. Fuchs B. and Mylonakis E. (2006). Using non-mammalian host to study fungal virulence and host defense. Curr. Opin Microbiol . 9: 346–351.
8. Mylonakis E. (2008). Galleria mellonella and the study of fungal pathogenesis: making the case for another genetically tractable model host. Mycopathol. 165: 1–3.
9. Bergin D., Brennan M.. and Kavanagh K. (2003). Fluctuations in haemocyte density and microbial load may be used as indicators of fungal pathogenicity in larvae of Galleria mellonella . Microb. Infect. 5: 1389–1395.
10. Bergin D., Murphy L., Keenan J., Clynes M. and Kavanagh K. (2006). Pre-exposure to yeast protects larvae of Galleria mellonella from a subsequent lethal infection by Candida albicans and is mediated by the increased expression of antimicrobial peptides. Microb. Infect. 8: 2105–2112.
11. Mowlds P., Coates C., Renwick J. and Kavanagh K. (2010). Dose-dependent cellular and humoral responses in Galleria mellonella larvae following β-glucan inoculation. Microb. Infect. 12: 146–153.
12. Tickoo S., and Russell S. (2002). Drosophila melanogaster as a model system for drug discovery and pathway screening. Curr Opin Pharmacol. 2: 555–60.
13. Hamamoto H., Kurokawa K., Kaito C., Kamura K., Manitra Razanajatovo I., Kusuhara H., Santa T., and Sekimizu K. (2004). Quantitative evaluation of the therapeutic effects of antibiotics using silkworms infected with human pathogenic microorganisms. Antimicrob. Agent. Chemother. 48: 774–779.
14. Hamamoto H., Tonioike A., Narushima K., Horie R., and Sekimizu K.(2009). Silkworm as a model animal to evaluate drug candidate toxicity and metabolism. Comp. Biochem. Physiol. 149: 334–339
15. Rowan R., Moran C., McCann M., and Kavanagh K. (2009). Use of Galleria mellonella larvae to evaluate the in vivo anti-fungal activity of (Ag2(mal)(phen)3). Biometals 22: 461–7.
16. Cytrnska, M., Mak, P., Zdybicka-Barabas, A., Suder, P., and Jacubowicz T. (2007). Purification and characterization of 8 peptides from Galleria mellonella immune haemolymph. Peptides 28:3: 533–546.
17. Shevchenko, A., Tomas, H., Havlis,J., Olsen, J.V., and Mann, M. (2006). In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocols 1: 2856–2860.