Modular gene over-expression strategies for candida albicans

Methods in Molecular Biology

Volumn 845, Issue , 2012, Pages 227-244

  Cabral, Vitor ^a,b   Chauvel, Murielle ^a,b   Firon, Arnaud ^a,b   Legrand, Mélanie ^a,b   Nesseir, Audrey ^a,b   Bachellier Bassi, Sophie ^a,b   Chaudhari, Yogesh ^c   Munro, Carol A ^c   d’Enfert, Christophe ^a,b  

^a INSTITUT PASTEUR   (France)

^b INRA USC 2019   (France)

^c UNIVERSITY OF ABERDEEN   (United Kingdom)

Author keywords

Gateway cloning; Genetic transformation; Integrative vectors; Over expression; Strain storage; TAP tagging; Western blotting

Indexed keywords

CANDIDA ALBICANS; GENE OVEREXPRESSION; GENETIC TRANSFORMATION; NONHUMAN; WESTERN BLOTTING; ARTICLE; FUNGAL GENOME; GENE EXPRESSION REGULATION; GENETIC ASSOCIATION; GENETICS; GENOMICS; METHODOLOGY; OPEN READING FRAME;

CANDIDA ALBICANS; GENE EXPRESSION REGULATION, FUNGAL; GENOME, FUNGAL; GENOME-WIDE ASSOCIATION STUDY; GENOMICS; OPEN READING FRAMES;

CANDIDA ALBICANS;

EID: 84858131020     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-539-8_15     Document Type: Chapter

References (20)

1. Sopko, R., Huang, D., Preston, N., Chua, G., Papp, B., Kafadar, K., Snyder, M., Oliver, S. G., Cyert, M., Hughes, T. R., Boone, C., and Andrews, B. (2006) Mapping pathways and phenotypes by systematic gene overexpression, Mol Cell 21, 319–330.
2. Chua, G., Morris, Q. D., Sopko, R., Robinson, M. D., Ryan, O., Chan, E. T., Frey, B. J., Andrews, B. J., Boone, C., and Hughes, T. R. (2006) Identifying transcription factor functions and targets by phenotypic activation, Proc Natl Acad Sci USA 103, 12045–12050.
3. Hiller, D., Sanglard, D., and Morschhauser, J. (2006) Overexpression of the MDR1 gene is sufficient to confer increased resistance to toxic compounds in Candida albicans, Antimicrob Agents Chemother 50, 1365–1371.
4. Leuker, C. E., Sonneborn, A., Delbruck, S., and Ernst, J. F. (1997) Sequence and promoter regulation of the PCK1 gene encoding phosphoenolpyruvate carboxykinase of the fungal pathogen Candida albicans, Gene 192, 235–240.
5. Nobile, C. J., Andes, D. R., Nett, J. E., Smith, F. J., Yue, F., Phan, Q. T., Edwards, J. E., Filler, S. G., and Mitchell, A. P. (2006) Critical role of Bcr1-dependent adhesins in C. albicans biofilm formation in vitro and in vivo, PLoS Pathog. 2, e63.
6. Nobile, C. J., Solis, N., Myers, C. L., Fay, A. J., Deneault, J. S., Nantel, A., Mitchell, A. P., and Filler, S. G. (2008) Candida albicans transcription factor Rim101 mediates pathogenic interactions through cell wall functions, Cell Microbiol 10, 2180–2196.
7. Nobile, C. J., Nett, J. E., Hernday, A. D., Homann, O. R., Deneault, J. S., Nantel, A., Andes, D. R., Johnson, A. D., and Mitchell, A. P. (2009) Biofilm matrix regulation by Candida albicans Zap1, PLoS biology 7, e1000133.
8. Park, Y. N., and Morschhauser, J. (2005) Tetracycline-inducible gene expression and gene deletion in Candida albicans, Eukaryot Cell 4, 1328–1342.
9. Fu, Y., Luo, G., Spellberg, B. J., Edwards, J. E., Jr., and Ibrahim, A. S. (2008) Gene overexpres-sion/suppression analysis of candidate virulence factors of Candida albicans, Eukaryot Cell 7, 483–492.
10. Saville, S. P., Lazzell, A. L., Monteagudo, C., and Lopez-Ribot, J. L. (2003) Engineered control of cell morphology in vivo reveals distinct roles for yeast and filamentous forms of Candida albicans during infection, Eukaryot. Cell 2, 1053–1060.
11. Saville, S. P., Thomas, D. P., and Lopez Ribot, J. L. (2006) A role for Efg1p in Candida albicans interactions with extracellular matrices, FEMS Microbiol Lett 256, 151–158.
12. Uppuluri, P., Pierce, C. G., Thomas, D. P., Bubeck, S. S., Saville, S. P., and Lopez-Ribot, J. L. (2010) The transcriptional regulator Nrg1p controls Candida albicans biofilm formation and dispersion, Eukaryot Cell 9, 1531–1537.
13. Carlisle, P. L., Banerjee, M., Lazzell, A., Monteagudo, C., Lopez-Ribot, J. L., and Kadosh, D. (2009) Expression levels of a filament-specific transcriptional regulator are sufficient to determine Candida albicans morphology and virulence, Proc Natl Acad Sci USA 106, 599–604.
14. Sahni, N., Yi, S., Daniels, K. J., Huang, G., Srikantha, T., and Soll, D. R. (2010) Tec1 mediates the pheromone response of the white phenotype of Candida albicans: insights into the evolution of new signal transduction pathways, PLoS biology 8, e1000363.
15. Murad, A. M., Lee, P. R., Broadbent, I. D., Barelle, C. J., and Brown, A. J. (2000) CIp10, an efficient and convenient integrating vector for Candida albicans, Yeast 16, 325–327.
16. Rigaut, G., Shevchenko, A., Rutz, B., Wilm, M., Mann, M., and Seraphin, B. (1999) A generic protein purification method for protein complex characterization and proteome exploration, Nat Biotechnol 17, 1030–1032.
17. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
18. Gillum, A. M., Tsay, E. Y., and Kirsch, D. R. (1984) Isolation of the Candida albicans gene for orotidine-5’-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations, Mol. Gen. Genet. 198, 179–182.
19. Firon, A., Aubert, S., Iraqui, I., Guadagnini, S., Goyard, S., Prevost, M. C., Janbon, G., and d’Enfert, C. (2007) The SUN41 and SUN42 genes are essential for cell separation in Candida albicans, Mol. Microbiol. 66, 1256–1275.
20. Taylor, R. G., Walker, D. C., and McInnes, R. R. (1993) E. coli host strains significantly affect the quality of small scale plasmid DNA preparations used for sequencing, Nucleic Acids Res 21, 1677–1678.