Calcium stabilizes the von Willebrand factor A2 domain by promoting refolding

Proceedings of the National Academy of Sciences of the United States of America

Volumn 109, Issue 10, 2012, Pages 3742-3747

  Xu, Amy J ^a,b   Springer, Timothy A ^a  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; VON WILLEBRAND FACTOR; VON WILLEBRAND FACTOR CLEAVING PROTEINASE;

ARTICLE; CALCIUM BINDING; CONFORMATIONAL TRANSITION; DENATURATION; ISOTHERMAL TITRATION CALORIMETRY; MELTING POINT; OPTICAL TWEEZERS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN REFOLDING; PROTEIN STABILITY; PROTEIN UNFOLDING; TENSILE STRENGTH;

ADAM PROTEINS; BINDING SITES; BIOPHYSICS; CALCIUM; CALORIMETRY; CIRCULAR DICHROISM; HEMOSTASIS; HUMANS; KINETICS; MOLECULAR CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEOLYSIS; TEMPERATURE; THERMODYNAMICS; VON WILLEBRAND FACTOR;

EID: 84857932056     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1121261109     Document Type: Article

References (26)

1. Springer TA (2011) Biology and physics of von Willebrand factor concatamers. J Thromb Haemost 9(Suppl 1):130-143.
2. Hoyer LW, Shainoff JR (1980) Factor VIII-related protein circulates in normal human plasma as high molecular weight multimers. Blood 55(6):1056-1059. (Pubitemid 10063385)
3. Schneider SW, et al. (2007) Shear-induced unfolding triggers adhesion of von Willebrand factor fibers. Proc Natl Acad Sci USA 104:7899-7903. (Pubitemid 47185848)
4. Sadler JE (2005) New concepts in von Willebrand disease. Annu Rev Med 56:173-191.
5. Sadler JE, Moake JL,Miyata T, George JN (2004) Recent advances in thrombotic thrombocytopenic purpura. Hematology Am Soc Hematol Educ Program 2004:407-423.
6. Sadler JE (2008) Von Willebrand factor, ADAMTS13, and thrombotic thrombocytopenic purpura. Blood 112:11-18.
7. Zhang Q, Zhou Y-F, Zhang C-Z, Springer TA (2009) Structural specializations of A2, a force-sensing domain in the ultralarge vascular protein von Willebrand factor. Proc Natl Acad Sci USA 106:9226-9231.
8. Dong JF, et al. (2002) ADAMTS-13 rapidly cleaves newly secreted ultralarge von Willebrand factor multimers on the endothelial surface under flowing conditions. Blood 100:4033-4039.
9. Zhang X, Halvorsen K, Zhang CZ, Wong WP, Springer TA (2009) Mechanoenzymatic cleavage of the ultralarge vascular protein, von Willebrand factor. Science 324:1330-1334.
10. Zhou M, et al. (2011) A novel calcium-binding site of von Willebrand factor A2 domain regulates its cleavage by ADAMTS13. Blood 117:4623-4631.
11. Jakobi AJ, Mashaghi A, Tans SJ, Huizinga EG (2011) Calcium modulates force sensing by the von Willebrand factor A2 domain. Nat Commun 2(385):1-9.
12. Cruz MA, Whitelock J, Dong JF (2003) Evaluation of ADAMTS-13 activity in plasma using recombinant von Willebrand factor A2 domain polypeptide as substrate. J Thromb Haemost 90(6):1204-1209. (Pubitemid 38004445)
13. Toumadje A, Alcorn SW, Johnson WC, Jr (1992) Extending CD spectra of proteins to 168 nm improves the analysis for secondary structures. Anal Biochem 200:321-331.
14. Boeckler FM, et al. (2008) Targeted rescue of a destabilized mutant of p53 by an in silico screened drug. Proc Natl Acad Sci USA 105:10360-10365.
15. Greenfield NJ (2006) Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions. Nat Protoc 1:2527-2535.
16. Ainavarapu SR, et al. (2007) Contour length and refolding rate of a small protein controlled by engineered disulfide bonds. Biophys J 92:225-233. (Pubitemid 46048430)
17. Dudko OK, Hummer G, Szabo A (2006) Intrinsic rates and activation free energies from single-molecule pulling experiments. Phys Rev Lett 96:108101-108104.
18. Evans E, Ritchie K (1997) Dynamic strength of molecular adhesion bonds. Biophys J 72:1541-1555. (Pubitemid 27133095)
19. Daggett V, Li A, Itzhaki LS, Otzen DE, Fersht AR (1996) Structure of the transition state for folding of a protein derived from experiment and simulation. J Mol Biol 257(2):430-440. (Pubitemid 26114044)
20. Krauss N, et al. (2008) The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops. Proteins 73:552-565. (Pubitemid 352788640)
21. Dudko OK, Hummer G, Szabo A (2008) Theory, analysis, and interpretation of single-molecule force spectroscopy experiments. Proc Natl Acad Sci USA 105:15755-15760.
22. Wu T, Lin J, Cruz MA, Dong JF, Zhu C (2010) Force-induced cleavage of single VWF A1A2A3-tridomains by ADAMTS-13. Blood 115:370-378.
23. Ying J, Ling Y, Westfield LA, Sadler JE, Shao JY (2010) Unfolding the A2 domain of von Willebrand factor with the optical trap. Biophys J 98:1685-1693.
24. Feys HB, et al. (2006) ADAMTS-13 plasma level determination uncovers antigen absence in acquired thrombotic thrombocytopenic purpura and ethnic differences. J Thromb Haemost 4:955-962.
25. Smith SB, Cui Y, Bustamante C (1996) Overstretching B-DNA: The elastic response of individual double-stranded and single-stranded DNA molecules. Science 271:795-799.
26. Kim J, Zhang C, Zhang X, Springer TA (2010) A mechanically stabilized receptor-ligand flex-bond important in the vasculature. Nature 466:992-995.