Cloning and overexpression of ketopantoic acid reductase gene from Stenotrophomonas maltophilia and its application to stereospecific production of D-pantoic acid

Applied Microbiology and Biotechnology

Volumn 93, Issue 4, 2012, Pages 1619-1625

  Si, Dayong ^a   Urano, Nobuyuki ^a,b   Shimizu, Sakayu ^a,c   Kataoka, Michihiko ^a,b  

^a KYOTO UNIVERSITY   (Japan)

^b Osaka Prefecture University   (Japan)

^c Ukyo ku   (Japan)

Author keywords

Bioreduction; d pantoic acid; Ketopantoic acid reductase; Stenotrophomonas maltophilia

Indexed keywords

AMINO ACID RESIDUES; AMINO ACID SEQUENCE; BIOREDUCTIONS; COFACTOR REGENERATION; CONVERSION YIELD; E. COLI; EXPRESSION VECTORS; KETOPANTOIC ACID REDUCTASE; N-TERMINAL AMINO ACID SEQUENCE; OPEN READING FRAME; OVER-EXPRESSION; REDUCTASE GENES; STENOTROPHOMONAS MALTOPHILIA; STEREOSPECIFIC; STEREOSPECIFIC REDUCTIONS; SUBSTRATE CONCENTRATIONS; TRANSFORMANT CELLS;

AMINO ACIDS; CLONING; ENZYMES; ESCHERICHIA COLI; GENE ENCODING;

GENE EXPRESSION;

AMINO ACID; DEXTRO PANTOIC ACID; KETOPANTOIC ACID REDUCTASE; OXIDOREDUCTASE; SHORT CHAIN DEHYDROGENASE; UNCLASSIFIED DRUG;

AMINO ACID; CLONE; COLIFORM BACTERIUM; GENE EXPRESSION;

AMINO ACID SEQUENCE; ARTICLE; BIOTRANSFORMATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; GENE OVEREXPRESSION; GENE SEQUENCE; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; SEQUENCE ANALYSIS; STENOTROPHOMONAS MALTOPHILIA; STEREOSPECIFICITY;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; HYDROXYBUTYRATES; KETO ACIDS; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; STENOTROPHOMONAS MALTOPHILIA;

ESCHERICHIA COLI; STENOTROPHOMONAS MALTOPHILIA;

EID: 84857922396     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3664-x     Document Type: Article

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