Molecular identification of the microbial diversity in two sequencing batch reactors with activated sludge

Applied Microbiology and Biotechnology

Volumn 93, Issue 4, 2012, Pages 1725-1734

  Denecke, Martin ^a   Eilmus, Sascha ^a   Röder, Nadine ^a   Roesch, Christopher ^b   Bothe, Hermann ^b  

^a UNIVERSITY OF DUISBURG ESSEN   (Germany)

^b UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Activated sludge; Intermediate disturbance hypothesis (IDH); Microbial diversity; Sequencing batch reactor (SBR); tRFLP

Indexed keywords

ACTIVATED SLUDGE; INTERMEDIATE DISTURBANCE HYPOTHESIS; MICROBIAL DIVERSITY; SEQUENCING BATCH REACTORS; TRFLP;

CHEMICAL OXYGEN DEMAND; DEGRADATION; FORESTRY; MICROORGANISMS;

ACTIVATED SLUDGE PROCESS;

NITROGEN; ORGANIC CARBON;

ACTIVATED SLUDGE; ANOXIC CONDITIONS; BIODEGRADATION; BIOMASS; BIOREACTOR; CHEMICAL OXYGEN DEMAND; COMMUNITY COMPOSITION; DISTURBANCE; ENZYME ACTIVITY; IDENTIFICATION METHOD; MICROBIAL ACTIVITY; MICROBIAL COMMUNITY; MOLECULAR ANALYSIS; ORGANIC CARBON; SPECIES DIVERSITY; TAXONOMY;

ACTIVATED SLUDGE; AEROBIC REACTOR; ANAEROBIC REACTOR; ARTICLE; BACTERIUM; BIOMASS PRODUCTION; CHEMICAL OXYGEN DEMAND; COMMUNITY STRUCTURE; DEGRADATION; ECOSYSTEM; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; MICROBIAL IDENTIFICATION; NITROGEN METABOLISM; NONHUMAN; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RESTRICTION MAPPING; SEQUENCING BATCH REACTOR; SPECIES RICHNESS; TAXONOMY;

AEROBIOSIS; BIOMASS; BIOREACTORS; BIOTA; DNA FINGERPRINTING; METAGENOME; NITROGEN COMPOUNDS; ORGANIC CHEMICALS; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SEWAGE; WATER PURIFICATION;

EID: 84857920156     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3474-1     Document Type: Article

References (44)

1. Abwasserverordnung (1997) Verordnung über Anforderungen an das Einleiten von Abwasser in Gewässer, Bundesgesetzblatt, Neufassung vom 17. Juni 2004, I, 1108-1184
2. Amann RI, Ludwig W, Schleifer KH (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59:143-169
3. Blackburne R, Vadivelua VM, Yuana Z, Keller J (2007) Kinetic characterization of an enriched Nitrospira culture with comparison to Nitrobacter. Water Res 41:3033-3042 (Pubitemid 46935803)
4. Bottari BB, Ercolini D, Gatti M, Neviani E (2006) Application of FISH technology for microbial analysis: current state and prospects. Appl Microbial Biotech 73:485-494 (Pubitemid 350262625)
5. Bramucci M, Nagarajan V (2006) Bacterial communities in industrial wastewater bioreactors. Curr Opinion Microbiol 9:275-278 (Pubitemid 43817619)
6. Cabezas A, Draper P, Etchebehere C (2009) Fluctuation of microbial activities after influent load variations in a full-scale SBR: recovery of the biomass after starvation. Appl Microbiol Biotech 84:1191-1202
7. Chen CL,Wu JH, Tseng IC, Liang TM, Liu WT (2009) Characterization of active microbes in a full scale anaerobic fluidized bed reactor treating phenolic wastewater. Microbes Environ 24:144-153
8. Chudoba J, Grau P, Ottova Y (1973) Control of activated sludge filamentous bulking. I. Effect of hydraulic regime or degree of mixing in an aeration tank. Water Res 7:163-172
9. Connell JH (1978) Diversity in tropical rain forests and coral reefs. Science 199:1302-1310
10. Crocetti GR, Hugenholtz P, Bond PL, Schuler A, Keller J, Jenkins D, Blackall LA (2000) Identification of phosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantification. Appl Environ Microbiol 66:1175-1182 (Pubitemid 30130043)
11. Daims H, Taylor MW, Wagner M (2006) Wastewater treatment: a model system for microbial ecology. Trends Biotechnol 24:483-489 (Pubitemid 44537455)
12. Eilmus S, Heil M (2009) Bacterial associates of arboreal ants and their putative functions in an obligate ant-plant mutualism. Appl Environ Microbiol 75:4324-4332
13. Eilmus S, Rösch C, Bothe H (2007) Prokaryotic life in a potash-polluted marsh with emphasis on N-metabolizing microorganisms. Environ Pollut 146:478-491 (Pubitemid 46281393)
14. Eschenhagen M, Schuppler M, Röske I (2003) Molecular characterization of the microbial community in two activated sludge systems for the advanced treatment of domestic effluents. Water Res 37:3224-3232 (Pubitemid 36675372)
15. Felfoldi T, Szekely AJ, Goral R, Barkacs K, Scheirich D, Andras J, Racz A, Marialigeti K (2010) Polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent. Bioresource Technol 101:3406-3410
16. Forney LF, Liu W-T, Guckert JB, Kumagai Y, Namkung E, Nishihara T, Larson RL (2001) Structure of microbial communities in activated sludge: potential implications for assessing the biodegradability of chemicals. Ecotoxicol Environ Safety, section B 49:40-53
17. Goel R, Mino T, Satoh H, Matsuo T (1998) Enzyme activities under anaerobic and aerobic conditions in activated sludge sequencing batch reactor. Water Res 32:2081-2088 (Pubitemid 28330497)
18. Hao Y, Winans SC, Glick B, Charles TC (2010) Identification and characterization of new LuxR/LuxI-type quorum sensing systems from metagenomics libraries. Environ Microbiol 12:105-117
19. Jetten M, Schmid M, Van de Pas-Schoonen K, Sinninghe Damsté JS, Strous S (2005) Anammox organisms: enrichment, cultivation and environmental analysis. Environ Microbiol 397:39-57
20. Juteau P, Tremblay D, Villemur R, Bisaillon J-G, Beaudet R (2005) Analysis of the bacterial community inhabiting an aerobic thermophilic sequencing batch reactor (AST-SBR) treating swine waste. Environ Biotechn 66:115-122
21. Kent AD, Smith DJ, Benson BJ, Triplett EW (2003) Web-based phylogenetic assignment tool for analysis of terminal restriction fragment length polymorphism profiles of microbial communities. Appl Environ Microbiol 69:6768-6776 (Pubitemid 37420216)
22. Koenig A, Zhang T, Liu L-H, Fang HHP (2005) Microbial community and biochemistry process in autosulfurotrophic denitrifying biofilm. Chemosphere 58:1041-1047 (Pubitemid 40138631)
23. Kong YH, Xia Y, Nielsen JL, Nielsen PH (2007) Structure and function of the microbial community in a full-scale enhanced biological phosphorus removal plant. Microbiology 153:4061-4073
24. Li HY, Yang M, Zhang Y, Liu XC, Gao MC, Kamagata Y (2005) Comparison of nitrification performance and microbial community between submerged membrane bioreactor and conventional activated sludge system. Water Sci Technol 51:535-543
25. Lin CK, Katayama Y, Hosomi M, Murakami A, Okada M (2003) The characteristics of the bacterial community structure and population dynamics for phosphorus removal in SBR activated sludge processes. Water Res 37:2944-2952 (Pubitemid 36593181)
26. Loy A, Daims H, Wagner W (2002) Activated sludge - molecular techniques for determining community composition. In: Bitton G (ed) Encyclopedia of environmental microbiology, vol 1. Wiley, New York, pp 26-43
27. Marchesi JR, Sato T, Weightman A, Martin TA, Fry JC, Hiom SJ, Wade WG (1998) Design and evaluation of useful bacteriumspecific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64:795-799 (Pubitemid 28079862)
28. Okubo Y, Futamata H, Hiraishi A (2006) Characterization of phototrophic purple nonsulfur bacteria forming colored microbial mats in a swine wastewater ditch. Appl Environ Microbiol 72:6225-6233 (Pubitemid 44414656)
29. 2-fixing, and total-community bacteria by terminal restriction fragment length polymorphism analysis using multiple restriction enzymes. Appl Environ Microbiol 71:2026-2035
30. Rösch C, Bothe H (2009) Diversity of total, nitrogen-fixing and denitrifying bacteria in an acid forest soil. Eur J Soil Sciences 60:883-894
31. Schütte UME, Abdo Z, Bent SJ, Shyu C, Williams CJ, Pierson JD, Forney LJ (2008) Advances in the use of terminal restriction fragment length polymorphism (T-RFLP) analysis of the 16S rRNA genes to characterize microbial communities. Appl Microbiol Biotechnol 80:365-380
32. Shin SG, Han G, Lim J, Lee C, Hwang S (2010) A comprehensive microbial insight into two-stage anaerobic digestion of food waste-recycling wastewater. Water Research: 44:4838-4849
33. Stres B, Tiedje JM, Murovec B (2009) BEsTRF: a tool for optimal resolution of terminal-restriction fragment length polymorphism analysis based on user-defined primer-enzyme-sequence-databases. Bioinformatics 26:1556-1558
34. Tomai CM, Annessini MC, Luberti R, Cento G, Senia A (2003) Kinetics of 4-nitrophenol biodegradation in a sequencing batch reactor. Water Res 37:3803-3814 (Pubitemid 36957931)
35. Tomlinson EJ, Chambers B (1979) Method for prevention of bulking in activated sludge. Water Poll Contr 78:524
36. Torsvik V, Goksøyr J, Daae FL (1990) High diversity in DNA of soil bacteria. Appl Environ Microbiol 56:782-787 (Pubitemid 20088454)
37. Wagner M, Amann R, Lemmer H, Schleifer K-H (1993) Probing activated sludge with proteobacteria-specific oligonucleotides: inadequacy of culture-dependent methods for describing microbial community structures. Appl Environ Microbiol 59:1520-1525 (Pubitemid 23141165)
38. Wagner M, Amann RI (1996) Die Anwendung von in situ- Hybridisierungssonden zur Aufklärung von Struktur und Dynamik der mikrobiellen Biozönosen in der Abwasserreinigung. In: Lemmer H, Flemming H-C, Griebe T (eds) Ökologie der Abwasserreinigung. Springer, Berlin, pp 93-110
39. Wagner M, Erhart R, Manz W, Amann R, Lemmer H, Wedi D, Schleifer K-H (1994) Development of a rRNA-targeted oligonucleotide probe for the genus Acinetobacter and its application for in situ monitoring of activated sludge. Appl Environ Microbiol 60:792-800 (Pubitemid 24077965)
40. Wagner M, Loy A (2002) Bacterial community composition and function in sewage treatment plants. Curr Opin Biotechn 13:218-227 (Pubitemid 35299541)
41. Wang Q, Garrity GM, Tiedje JM, Cole JR (2007) Native Bayesian classifier for rapid assignment of rRNS sequences into bacterial taxonomy. Appl Environ Microbiol 73:5261-5267 (Pubitemid 47326658)
42. Wilderer PA, Silverstein JA (1983) Bedeutung periodischer Stress- Bedingungen für die Wirksamkeit biologischer Klärverfahren. GWF Wasser Abwasser 124:546-552 (Pubitemid 14141145)
43. Wilen B-M, Onuki M, Hermansson M, Lumley D, Mino T (2008) Microbial community structure in activated sludge floc analysed by fluorescence in situ hybridization and its relation to floc stability. Water Research 42:2300-2308
44. Woolard CR, Irvine RL (1995) Treatment of hypersaline wastewater in the sequencing batch reactor. Water Res 29:1159-1168