Comparative epigenetic analysis of Oct4 regulatory region in RA-induced differentiated NT2 cells under adherent and non-adherent culture conditions

Molecular and Cellular Biochemistry

Volumn 363, Issue 1-2, 2012, Pages 129-134

  Favaedi, Raha ^a   Shahhoseini, Maryam ^a   Akhoond, Mohammad Reza ^a  

^a ROYAN INSTITUTE FOR REPRODUCTIVE BIOMEDICINE   (Iran)

Author keywords

Culture condition; Differentiation; Epigenetic; Oct4

Indexed keywords

HISTONE H3; OCTAMER TRANSCRIPTION FACTOR 4;

ACETYLATION; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL SUSPENSION; COMPARATIVE STUDY; DOWN REGULATION; EMBRYONAL CARCINOMA STEM CELL; EPIGENETICS; GENETIC ANALYSIS; GENETIC REGULATION; HISTONE MODIFICATION; HUMAN; HUMAN CELL; METHYLATION; PROTEIN EXPRESSION;

ACETYLATION; CARCINOMA, EMBRYONAL; CELL ADHESION; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE, TUMOR; CHROMATIN IMMUNOPRECIPITATION; DOWN-REGULATION; EPIGENESIS, GENETIC; GENE EXPRESSION REGULATION, NEOPLASTIC; HISTONES; HUMANS; LYSINE; METHYLATION; OCTAMER TRANSCRIPTION FACTOR-3; REAL-TIME POLYMERASE CHAIN REACTION; REGULATORY SEQUENCES, NUCLEIC ACID; TIME FACTORS; TRETINOIN;

EID: 84857907795     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-011-1165-y     Document Type: Article

References (24)

1. Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Scholer H, Smith A (1998) Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 95:379-391 (Pubitemid 28507326)
2. Pan GJ, Chang ZY, Schöler HR, Pei D (2002) Stem cell pluripotency and transcription factor Oct4. Cell Res 12:321-329 (Pubitemid 135708344)
3. Shi G, Jin Y (2010) Role of Oct4 in maintaining and regaining stem cell pluripotency. Stem Cell Res Ther 1:39
4. Schöler HR, Ruppert S, Suzuki N, Chowdhury K, Gruss P (1990) New type of POU domain in germ line-specific protein Oct-4. Nature 344:435-439
5. Phillips K, Luisi B (2000) The virtuoso of versatility: POU proteins that flex to fit. J Mol Biol 302:1023-1039
6. Yeom Y, Fuhrmann G, Ovitt C, Brehm A, Ohbo K, Gross M, Hubner K, Scholer H (1996) Germline regulatory element of Oct4 specific for the totipotent cycle of embryonal cells. Integrated Ann Indexes 122:881-894 (Pubitemid 26095994)
7. Nordhoff V, Hubner K, Bauer A, Orlova I, Malapetsa A, Scholer HR (2001) Comparative analysis of human, bovine, and murine Oct4 upstream promoter sequences. Mamm Genome 12:309-317 (Pubitemid 32242892)
8. Ovitt CE, Scholer HR (1998) The molecular biology of Oct-4 in the early mouse embryo. Mol Human Reprod 4:1021-1031 (Pubitemid 28508992)
9. Okazawa H, Okamoto K, Ishino F, Ishino-Kaneko T, Takeda S, Toyoda Y, Muramatsu M, Hamada H (1991) The oct3 gene, a gene for an embryonic transcription factor, is controlled by a retinoic acid repressible enhancer. EMBO J 10:2997-3005 (Pubitemid 21905318)
10. Schoorlemmer J, van PA, van Den Eijnden M, Jonk L, Pals C, Kruijer W (1994) Characterization of a negative retinoic acid response element in the murine Oct4 promoter. Mol Cell Biol 14:1122-1136
11. Ben-Shushan E, Pikarsky F, Klar A, Bergman Y (1993) Extinction of Oct-3/4 gene expression in embryonal carcinoma*fibroblast somatic cell hybrids is accompanied by changes in the methylation status, chromatin structure, and transcriptional activity of the Oct-3/4 upstream region. Mol Cell Biol 13:891-901 (Pubitemid 23040254)
12. Ben-Shushan E, Sharir H, Pikarsky F, Bergman Y (1995) A dynamic balance between ARP-1/COUP-TFII, EAR-3/Coup-TFI, and retinoic acid receptor: retinoid X receptor hetero dimers regulates Oct3/4 expression in embryonal carcinoma cells. Mol Cell Biol 15:1034-1048
13. Shahoseini M, Taei A, Mehrjardi NZ, Salekdeh GH, Baharvand H (2010) Epigenetic analysis of human embryonic cells during RA-induced neural differentiation. Biochem Cell Biol 88:527-538
14. Feldman N, Gerson A, Fang J, Li E, Zhang Y, Shinkai Y, Cedar H, Bergman Y (2006) G9a-mediated irreversible epigenetic inactivation of Oct-3/4 during early embryogenesis. Nat Cell Biol 8:188-194
15. Kellner S, Kikyo N (2010) Transcriptional regulation of the Oct4 gene, a master gene of pluripotency. Histol Histopathol 25:405-412
16. Hake SB, Garcia BA, Duncan EM, Kauer M, Dellaire G, Shabanowitz J, Bazett-Jones DP, Allis CD, Hunt DF (2006) Expression patterns and post-translational modifications associated with mammalian histone H3 variants. J Biol Chem 281:559-568 (Pubitemid 43671219)
17. Shahhoseini M, Favaedi R, Baharvand H, Sharma V, Stunnenberg HG (2010) Evidence for a dynamic role of the linker histone variant H1x during retinoic acid-induced differentiation of NT2 cells. FEBS Lett 584:4661-4664
18. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCtmethod. Methods 25:402-408 (Pubitemid 34164012)
19. 2ChIP, a quick and quantitative chromatin immunoprecipitation assay, unravels epigenetic dynamics of developmentally regulated genes in human carcinoma cells. Stem Cells 25:1037-1046
20. Przyborski SA, Christie VB, Hayman MW, Stewart R, Horrocks GM (2004) Human embryonal carcinoma stem cells: models of embryonic development in humans. Stem Cells Dev 13:400-408 (Pubitemid 39223291)
21. Przyborski SA, Smith S, Wood A (2003) Transcriptional profiling of neuronal differentiation by human embryonal carcinoma stem cells in vitro. Stem Cells 21:459-471 (Pubitemid 36793488)
22. Deb-Rinker P, Ly D, Jezierski A, Sikorska M, Walker PR (2005) Sequential DNA methylation of the Nanog and Oct-4 upstream regions in human NT2 cells during neuronal differentiation. J Biol Chem 280:6257-6260 (Pubitemid 40341170)
23. Horrocks GM, Lauder L, Stewart R, Przyborski SA (2003) Formation of neurospheres from human embryonal carcinoma stem cells. Biochem Biophys Res Commun 304:411-416 (Pubitemid 36444586)
24. Megiorni F, Mora B, Indovina P, Mazzilli MC (2005) Expression of neuronal markers during NTera2/cloneD1 differentiation by cell aggregation method. Neurosci Lett 373:105-110