A possible mechanism of basic fibroblast growth factor-promoted scarless wound healing: The induction of myofibroblast apoptosis

European Journal of Dermatology

Volumn 22, Issue 1, 2012, Pages 46-53

  Abe, Masatoshi ^a   Yokoyama, Yoko ^a   Ishikawa, Osamu ^a  

^a GUNMA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

Akt; Apoptosis; Basic fibroblast growth factor (bFGF); Rho family; Scarless wound healing

Indexed keywords

2 MORPHOLINO 8 PHENYLCHROMONE; 4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; ALPHA SMOOTH MUSCLE ACTIN; FIBROBLAST GROWTH FACTOR 2; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B; PROTEIN KINASE B INHIBITOR; RAC1 PROTEIN; RHO FACTOR; RHO KINASE; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; TRANSFORMING GROWTH FACTOR BETA;

APOPTOSIS; ARTICLE; CONTROLLED STUDY; FIBROBLAST; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOFLUORESCENCE MICROSCOPY; IN VITRO STUDY; MYOFIBROBLAST; PHENOTYPE; POLYACRYLAMIDE GEL ELECTROPHORESIS; SIGNAL TRANSDUCTION; WOUND HEALING;

ACTINS; AMIDES; APOPTOSIS; CELLS, CULTURED; CHROMONES; CICATRIX; EXTRACELLULAR MATRIX; FIBRILLAR COLLAGENS; FIBROBLAST GROWTH FACTOR 2; FIBROBLASTS; HUMANS; MORPHOLINES; MYOFIBROBLASTS; MYOSIN LIGHT CHAINS; PHOSPHATIDYLINOSITOL 3-KINASE; PHOSPHORYLATION; PYRIDINES; RAC1 GTP-BINDING PROTEIN; RHOA GTP-BINDING PROTEIN; SIGNAL TRANSDUCTION; TRANSFORMING GROWTH FACTOR BETA1; WOUND HEALING;

EID: 84857878920     PISSN: 11671122     EISSN: 19524013     Source Type: Journal    
DOI: 10.1684/ejd.2011.1582     Document Type: Article

References (40)

1. Abe M, Sogabe Y, Syuto T, Yokoyama Y, Ishikawa O. Evidence that PI3K, Rac Rho, and Rho kinase are involved in basic fibroblast growth factor-stimulated fibroblast-Collagen matrix contraction. J Cell Biochem 2007; 102: 503-9.
2. Mukhopadhyay A, Wong MY, Chan SY, et al. Syndecan-2 and decorin: proteoglycans with a difference-implications in keloid pathogenesis. J Trauma 2010;68:999-1008.
3. Tomasek JJ, Gabbiani G, Hinz B, Chaponnier C, Brown RA. Myofibroblasts and mechano-regulation of connective tissue remodelling. Nat Rev Mol Cell Biol 2002; 3: 349-63.
4. Miron-Mendoza M, Seemann J, Grinnell F. The differential regulation of cell motile activity through matrix stiffness and porosity in three dimensional collagen matrices. Biomaterials 2010; 31:6425-35.
5. Menezes GC, Miron-Mendoza M, Ho CH, Jiang H, Grinnell F. Oncogenic Ras-transformed human fibroblasts exhibit differential changes in contraction and migration in 3D collagen matrices. Exp Cell Res 2008; 314:3081-91.
6. Grinnell F. Fibroblast mechanics in three-dimensional collagen matrices. J Bodyw Mov Ther 2008; 12: 191-3.
7. Kolodney MS, Elson EL. Correlation of myosin light chain phosphorylation with isometric contraction of fibroblasts. J Biol Chem 1993; 268:23850-5.
8. Shreiber DI, Enever PA, Tranquillo RT. Effects of PDGF-bb on rat dermal fibroblast behavior in mechanically stressed and unstressed collagen and fibrin gels. Exp Cell Res 2001;266: 155-66.
9. Grinnell F, Ho CH, Lin YC, Skuta G. Differences in the regulation of fibroblast contraction of floating versus stressed collagen matrices. J Biol Chem 1999; 274:918-23.
10. Grinnell F, Ho CH, Tamariz E, Lee DJ, Skuta G. Dendritic fibroblasts in three-dimensional collagen matrices. Mol Biol Cell 2003; 14: 384-95.
11. Abe M, Ho CH, Kamm KE, Grinnell F. Different molecular motors mediate platelet-derived growth factor and lysophosphatidic acid-stimulated floating collagen matrix contraction. J Biol Chem 2003; 278:47707-12.
12. Grinnell F, Ho CH. Transforming growth factor β stimulates fibroblast-collagen matrix contraction by different mechanisms in mechanically loaded and unloaded matrices. Exp Cell Res 2002; 273:248-55.
13. KondoT, Ishida Y. Molecular pathology of wound healing. Forensic Sci Int 2010; 203:93-8.
14. Ono I, Akasaka Y, Kikuchi R, et al. Basic fibroblast growth factor reduces scar formation in acute incisional wounds. Wound Rep Reg 2007; 15:617-23.
15. Gospodarowicz D. Isolation and characterization of acidic and basic fibroblast growth factor. Methods Enzymol 1987; 147: 106-19.
16. Gospodarowicz D, Ferrara N, Schweigerer L, Neufeld G. Structural characterization and biological functions of fibroblast growth factor. Endocr Rev 1987; 8: 95-114.
17. Makino T, Jinnin M, Muchemwa FC, et al. Basic fibroblast growth factor stimulates the proliferation of human dermal fibroblasts via the ERK1/2 and JNK pathways. Br J Dermatol 2010; 162:717-23.
18. Kanazawa S, Fujiwara T, Matsuzaki S, et al. bFGF regulates PI3-kinase-Rac1-JNK pathway and promotes fibroblast migration in wound healing. PLoS One 2010; 5: e12228.
19. Ono I. The effects of basic fibroblast growth factor (bFGF) on the breaking strength of acute incisional wounds. J Dermatol Sci 2002; 29: 104-13.
20. Moulin V, Castilloux G, Jean A, Garrel DR, Auger FA, Germain L. In vitro models to study wound healing fibroblasts. Burns 1996; 22: 359-62.
21. Petri JB, Saalbach A, HauptB, PiererM, Hausten UF, Herrmann K. In vitro analysis of adhesion molecule expression and gel contraction of human granulation tissue fibroblasts. Wound Rep Reg 1997; 5: 69-76.
22. Crider BJ, Risinger GM, Haaksma CJ, Howard EW, Tomasek JJ. Myocardin-Related Transcription Factors A and B Are Key Regulators of TGF-(i1-Induced Fibroblast to Myofibroblast Differentiation. J Invest Dermatol 2011.[Epub ahead of print].
23. Vyas B, Ishikawa K, Duflo S, Chen X, Thibeault SL. Inhibitory effects of hepatocyte growth factor and interleukin-6 on transforming growth factor-beta1 mediated vocal fold fibroblast-myofibroblast differentiation. Ann Otol Rhinol Laryngol 2010; 119: 350-7.
24. Serini G, Gabbiani G. Mechanisms of myofibroblast activity and phenotypic modulation. Exp Cell Res 1999; 250: 273-83.
25. Jester JV, Petroll WM, Cavanagh HD. Corneal stromal wound healing in refractive surgery: The role of myofibroblasts. Prog Retin Eye Res 1999; 18:311-56.
26. Powell DW, Mifflin RC, Valentich JD, Crowe SE, Saada JL, West AB. Myofibroblasts. I. Paracrine cells important in health and disease. Am J Physiol 1999; 277: C1-9.
27. Arora PD, Narani N, McCulloch CA. The compliance of collagen gels regulates transforming growth factor-beta induction of alpha-smooth muscle actin in fibroblasts. Am J Pathol 1999; 154: 871-82.
28. Vaughan MB, Howard EW, Tomasek JJ. Transforming growth factor-beta1 promotes the morphological and functional differentiation of the myofibroblast. Exp Cell Res 2000; 257: 180-9.
29. Chang ZF, Lee HH. RhoA signaling in phorbol ester-induced apop-tosis. J Biomed Sci 2006; 13:173-80.
30. Brickley-Parsons D, Glimcher MJ, Smith RJ, Albin R, Adams JP. Biochemical changes in the collagen of the palmar fascia in patients with Dupuytren's disease. J Bone Joint Surg Am 1981;63: 787-97.
31. Greenhalgh DG. The role of apoptosis in wound healing. Int J Biochem Cell Biol 1998; 30: 1019-30.
32. Akasaka Y, Ito K, Fujita K, et al. Activated caspase expression and apoptosis increase in keloids: cytochrome c release and caspase-9 activation during the apoptosis of keloid fibroblast lines. Wound Rep Reg 2005;13:373-82.
33. Akasaka Y, Ishikawa Y, Ono I, et al. Enhanced expression of caspase-3 in hypertrophic scars and keloid: induction of caspase-3 and apoptosis in keloid fibroblasts in vitro. Lab Invest 2000; 80: 345-57.
34. Akasaka Y, Fujita K, Ishikawa Y, et al. Detection of apoptosis inkeloids and a comparative study on apoptosis between keloids, hypertrophic scars, normal healed flat scars, and dermatofibroma. Wound Rep Reg 2001;9:501-6.
35. Brown DL, Kao WW, Greenhalgh DG. Apoptosis down-regulates inflammation under the advancing epithelial wound edge: delayed patterns in diabetes and improvement with topical growth factors. Surgery 1997; 121:372-80.
36. Lamme EN, Van Leeuwen RT, Brandsma K, Van Marle J, Middelkoop E. Higher numbers of autologous fibroblasts in an artificial dermal substitute improve tissue regeneration and modulate scar tissue formation. J Pathol 2000; 190: 595-603.
37. Datta SR, Dudek H, Tao X, et al. Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 1997; 91:231-41.
38. Ramos C, Montano M, Becerril C, et al. Acidic fibroblast growth factor decreases alpha-smooth muscle actin expression and induces apoptosis in human normal lung fibroblasts. Am J Physiol Lung Cell Mol Physiol 2006; 291: L871-879.
39. Tiede S, Ernst N, Bayat A, Paus R, Tronnier V, Zechel C. Basic fibroblast growth factor: a potential new therapeutic tool for the treatment of hypertrophic and keloid scars. Ann Anat 2009; 191: 33-44.
40. Lohmeyer JA, Liu F, Krüger S, Lindenmaier W, Siemers F, Machens HG. Use of gene-modified keratinocytes and fibroblasts to enhance regeneration in a full skin defect. Langenbecks Arch Surg 2011; 396:543-50.