In vivo labeling of cortical astrocytes with sulforhodamine 101 (SR101)

Cold Spring Harbor Protocols

Volumn 7, Issue 3, 2012, Pages 326-334

  Nimmerjahn, Axel ^a   Helmchen, Fritjof ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; SULFORHODAMINE 101; UNCLASSIFIED DRUG; COLORING AGENT; RHODAMINE;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; ASTROCYTE; BRAIN CORTEX; CELL LABELING; FLUORESCENCE MICROSCOPY; GAP JUNCTION; IN VIVO STUDY; MOUSE; NONHUMAN; RELIABILITY; ANIMAL; BRAIN; CYTOLOGY; METABOLISM; METHODOLOGY; RODENT; STAINING;

RODENTIA;

ANIMALS; ASTROCYTES; BRAIN; COLORING AGENTS; CYTOLOGICAL TECHNIQUES; MICROSCOPY, FLUORESCENCE; RHODAMINES; RODENTIA; STAINING AND LABELING;

EID: 84857849044     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot068155     Document Type: Article

References (29)

1. 2+ signals in situ and in vivo. J Neurosci Methods 181: 212-226.
2. Bushong EA, Martone ME, Ellisman MH. 2004. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci 22: 73-86.
3. Cahoy JD, Emery B, Kaushal A, Foo LC, Zamanian JL, Christopherson KS, Xing Y, Lubischer JL, Krieg PA, Krupenko SA, et al. 2008. A transcrip-tome database for astrocytes, neurons, and oligodendrocytes: A new resource for understanding brain development and function. J Neurosci 28: 264-278.
4. Clay GO, Schaffer CB, Kleinfeld D. 2007. Large two-photon absorptivity of hemoglobinintheinfrared rangeof780-880 nm. JChemPhys 126: 025102.
5. Colin A, Faideau M, Dufour N, Auregan G, Hassig R, Andrieu T, Brouillet E, Hantraye P, Bonvento G, Deglon N. 2009. Engineered lentiviral vector targeting astrocytes in vivo. Glia 57: 667-679.
6. Ehinger B, Zucker CL, Bruun A, Adolph A. 1994. In vivo staining of oligo-dendroglia in the rabbit retina. Glia 10: 40-48.
7. Garaschuk O, Milos R-I, Konnerth A. 2006. Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo. Nat Protoc 1: 380-386.
8. Göbel W, Kampa BM, Helmchen F.2007.Imaging cellular network dynamics in three dimensions using fast 3D laser scanning. Nat Methods 4: 73-79.
9. Hirrlinger PG, Scheller A, Braun C, Quintela-Schneider M, Fuss B, Hirrlin-ger J, Kirchhoff F. 2005. Expression of reef coral fluorescent proteins in the central nervous system of transgenic mice. Mol Cell Neurosci 30: 291-303.
10. Hoogland TM, Kuhn B, Göbel W, Huang W, Nakai J, Helmchen F, Flint J, Wang SS. 2009. Radially expanding transglial calcium waves in the intact cerebellum. Proc Natl Acad Sci 106: 3496-3501.
11. Kafitz KW, Meier SD, Stephan J, Rose CR. 2008. Developmental profile and properties of sulforhodamine 101-labeled glial cells in acute brain slices of rat hippocampus. J Neurosci Methods 169: 84-92.
12. Kang J, Kang N, Yu Y, Zhang J, Petersen N, Tian GF, Nedergaard M. 2010. Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons. Neuroscience 169: 1601-1609.
13. Keifer J, Vyas D, Houk JC. 1992. Sulforhodamine labeling of neural circuits engaged in motor pattern generation in the in vitro turtle brain-stem cerebellum. J Neurosci 12: 3187-3199.
14. Lavallee A, Pflieger JF. 2009. Developmental expression of spontaneous activity in the spinal cord of postnatal opossums, Monodelphis domes-tica: An anatomical study. Brain Res 1282: 1-9.
15. Matyash V, Kettenmann H. 2009. Heterogeneity in astrocyte morphology and physiology. Brain Res Rev 63: 2-10.
16. Meier SD, Kafitz KW, Rose CR. 2008. Developmental profile and mechanisms of GABA-induced calcium signaling in hippocampal astrocytes. Glia 56: 1127-1137.
17. 2+ imaging of local neuronal circuits. Neuron 53: 789-803.
18. Nguyen QT, Tsai PS, Kleinfeld D. 2006. MPScope: A versatile software suite for multiphoton microscopy. J Neurosci Methods 156: 351-359.
19. Nimmerjahn A. 2009. Astrocytes going live: Advances and challenges. J Physiol 587: 1639-1647.
20. Nimmerjahn A, Kirchhoff F, Kerr JND, Helmchen F. 2004. Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat Methods 1: 31-37.
21. Nimmerjahn A, Mukamel EA, Schnitzer MJ. 2009. Motor behavior activates Bergmann glial networks. Neuron 62: 400-412.
22. Nolte C, Matyash M, Pivneva T, Schipke CG, Ohlemeyer C, Hanisch UK, Kirchhoff F, Kettenmann H. 2001. GFAP promoter-controlled EGFP-expressing transgenic mice: A tool to visualize astrocytes and astroglio-sis in living brain tissue. Glia 33: 72-86.
23. Schummers J, Yu HB, Sur M. 2008. Tuned responses of astrocytes and their influence on hemodynamic signals in the visual cortex. Science 320: 1638-1643.
24. Thompson RJ, Zhou N, MacVicar BA. 2006. Ischemia opens neuronal gap junction hemichannels. Science 312: 924-927.
25. Thompson RJ, Jackson MF, Olah ME, Rungta RL, Hines DJ, Beazely MA, MacDonald JF, MacVicar BA. 2008. Activation of pannexin-1 hemichannels augments aberrant bursting in the hippocampus. Science 322: 1555-1559.
26. 2+ signaling evoked by sensory stimulation in vivo. Nat Neurosci 9: 816-823.
27. Winship IR, Murphy TH. 2008. In vivo calcium imaging reveals functional rewiring of single somatosensory neurons after stroke. J Neurosci 28: 6592-6606.
28. Xu G, Wang W, Kimelberg HK, Zhou M. 2009. Electrical coupling of astro-cytes in rat hippocampal slices under physiological and simulated ischemic conditions. Glia 58: 481-493.
29. Zuo Y, Lubischer JL, Kang H, Tian L, Mikesh M, Marks A, Scofield VL, Maika S, Newman C, Krieg P, et al. 2004. Fluorescent proteins expressed in mouse transgenic lines mark subsets of glia, neurons, macro-phages, and dendritic cells for vital examination. J Neurosci 24: 10999-11009.