Long-term time-lapse imaging of developing hippocampal neurons in culture

Cold Spring Harbor Protocols

Volumn 7, Issue 3, 2012, Pages 335-339

  Kaech, Stefanie ^a   Huang, Chun Fang ^a   Banker, Gary ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; KINESIN; PHOTOPROTEIN;

ANIMAL CELL; ARTICLE; AUTOMATION; CULTURE MEDIUM; CYTOSKELETON; DENDRITE; GENETIC TRANSFECTION; HIPPOCAMPUS; IMAGING SYSTEM; MICROSCOPY; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NERVE CELL GROWTH; NERVE FIBER; NONHUMAN; PROTEIN EXPRESSION; TIME LAPSE IMAGING; ANIMAL; CELL CULTURE; CULTURE TECHNIQUE; CYTOLOGY; FLUORESCENCE MICROSCOPY; GENETICS; METABOLISM; METHODOLOGY; MOUSE; NERVE CELL; PHYSIOLOGY; RAT; REPORTER GENE; STAINING;

RODENTIA;

ANIMALS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CYTOLOGICAL TECHNIQUES; GENES, REPORTER; HIPPOCAMPUS; LUMINESCENT PROTEINS; MICE; MICROSCOPY, FLUORESCENCE; NEURONS; RATS; STAINING AND LABELING; TIME-LAPSE IMAGING;

EID: 84857848712     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot068239     Document Type: Article

References (4)

1. Jacobson C, Schnapp B, Banker GA. 2006. A change in the selective translo-cation of the Kinesin-1 motor domain marks the initial specification of the axon. Neuron 49: 797-804.
2. Kaech S, Banker G. 2006. Culturing hippocampal neurons. Nat Protoc 1: 2406-2415.
3. Kaech S, Huang C-F, Banker G. 2012a. General considerations for live imaging of developing hippocampal neurons in culture. Cold Spring Harb Protoc doi: 10.1101/pdb.ip068221.
4. Kaech S, Huang C-F, Banker G. 2012b. Short-term high-resolution imaging of developing hippocampal neurons in culture. Cold Spring Harb Protoc doi: 10.1101/pdb.prot068247.