Preparing individual Drosophila egg chambers for live imaging

Journal of Visualized Experiments

Volumn , Issue 60, 2012, Pages

  Weil, Timothy T ^a   Parton, Richard M ^a   Davis, Ilan ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Cytoskeleton; Drosophila oocytes; Issue 60; Molecular biology; mRNA localization; Rna injection; Wide field microscopy

Indexed keywords

EID: 84857816669     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3679     Document Type: Article

References (15)

1. Arias, A.M. Drosophila melanogaster and the development of biology in the 20th century. Methods Mol. Biol. 420, 1-25 (2008).
2. Parton, R.M., Valles, A.-M., Dobbie, I.D., & Davis, I. Pushing the Limits of Live Cell Imaging in Drosophila. In: Live Cell Imaging: A Laboratory Manual, Second Edition, Goldman, R.D., Swedlow, J.R., & Spector, D.L., eds., Cold Spring Harbor Laboratory Press., 387-418 (2010).
3. St. Johnston, D. Moving messages: the intracellular localization of mRNAs. Nat. Rev. Mol. Cell Biol. 6(5), 363-375 (2005).
4. Jansen, R. mRNA Localization: Message on the Move. Nat. Rev. Mol. Cell Biol. 2, 247-256 (2001).
5. Van De Bor, V., Hartswood, E., Jones, C., Finnegan, D., & Davis, I. gurken and the I factor retrotransposon RNAs share common localization signals and machinery. Dev. Cell. 9 (1), 51-62 (2005).
6. Delanoue, R., Herpers, B., Soetaert, J., Davis, I., & Rabouille, C. Drosophila Squid/hnRNP helps Dynein switch from a gurken mRNA transport motor to an ultrastructural static anchor in sponge bodies. Dev. Cell. 13 (4), 523-538 (2007).
7. Jaramillo, A.M., Weil, T.T., Goodhouse, J., Gavis, E.R., & Schupbach, T. The dynamics of fluorescently labeled endogenous gurken mRNA in Drosophila. J. Cell. Sci. 121 (Pt. 6), 887-894 (2008).
8. Forrest, K.M. &Gavis, E.R. Live imaging of endogenous mRNA reveals a diffusion and entrapment mechanism for nanos mRNA localization in Drosophila. Curr. Biol. 13, 1159-1168 (2003).
9. Bertrand, E., et al. Localization of ASH1 mRNA particles in living yeast. Mol. Cell. 2 (4), 437-445 (1998).
10. Spradling, A.C. Developmental genetics of oogenesis. In: The Development of Drosophila melanogaster., vol. 1, Bate, M. & Martinez-Arias A., eds., Cold Spring Harbor Press, Cold Spring Harbor, 1-70 (1993).
11. MacDougall, N., Clark, A., MacDougall, E., & Davis, I. Drosophila gurken (TGFalpha) mRNA localizes as particles that move within the oocyte in two dynein-dependent steps. Dev. Cell. 4 (3), 307-319 (2003).
12. Tekotte, H., Tollervey, D., & Davis, I. Imaging the migrating border cell cluster in living Drosophila egg chambers. Dev. Dyn. 236 (10), 2818-2824 (2007).
13. Weil, T.T., Parton, R., Davis, I., & Gavis, E.R. Changes in bicoid mRNA anchoring highlight conserved mechanisms during the oocyte-to-embryo transition. Curr. Biol. (18), 1055-1061 (2008).
14. Prasad, M., Jang, A.C., Starz-Gaiano, M., Melani, M., & Montell, D.J. A protocol for culturing Drosophila melanogaster stage 9 egg chambers for live imaging. Nat. Protoc. 2 (10), 2467-2473 (2007).
15. Morris, L.X. & Spradling, A.C. Long-term live imaging provides new insight into stem cell regulation and germline-soma coordination in the Drosophila ovary. Development. 138 (11), 2207-2215 (2011).