A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay

Journal of Microbiological Methods

Volumn 89, Issue 1, 2012, Pages 38-40

  Elwin, K ^a   Robinson, G ^a   Hadfield, S J ^a   Fairclough, H V ^b   Iturriza Gomara M ^c   Chalmers, R M ^a  

^a SINGLETON HOSPITAL   (United Kingdom)

^b ABERYSTWYTH UNIVERSITY   (United Kingdom)

^c PUBLIC HEALTH ENGLAND   (United Kingdom)

Author keywords

Cryptosporidium; DNA extraction; Real time PCR

Indexed keywords

DNA; GUANIDINE THIOCYANATE; RIBOSOME RNA; SILICON DIOXIDE; THIOCYANIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; AUTOMATION; CELL SUSPENSION; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; DIAGNOSTIC TEST; DNA EXTRACTION; DNA PURIFICATION; FECES ANALYSIS; GASTROENTERITIS; HUMAN; INTERMETHOD COMPARISON; LABORATORY TEST; MOLECULAR EPIDEMIOLOGY; NONHUMAN; OOCYTE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SEQUENCE; SEPARATION TECHNIQUE; SPIN COLUMN EXTRACTION; SPOROZOITE;

CLINICAL LABORATORY TECHNIQUES; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; DNA, PROTOZOAN; FECES; HUMANS; PARASITOLOGY; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

CRYPTOSPORIDIUM;

EID: 84857773468     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.02.006     Document Type: Article

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