A rapid FACS-based strategy to isolate human gene knockin and knockout clones

PLoS ONE

Volumn 7, Issue 2, 2012, Pages

  Mata, João F ^a   Lopes, Telma ^a   Gardner, Rui ^a   Jansen, Lars E T ^a  

^a INSTITUTO GULBENKIAN DE CIÊNCIA   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; CENTROMERE PROTEIN A; DNA; GUANINE; THYMINE; YELLOW FLUORESCENT PROTEIN; BACTERIAL PROTEIN; FLUORESCENT DYE; PHOTOPROTEIN; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ALLELE; ARTICLE; CENTROMERIC PROTEIN A GENE; CONTROLLED STUDY; EXON; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE ACTIVATED CELL SORTING OF TARGETED CLONE; GENE; GENE CASSETTE; GENE CONSTRUCT; GENE DELETION; GENE DISRUPTION; GENE ISOLATION; GENE LOCUS; GENE SILENCING; GENE TARGETING; GENE VECTOR; HUMAN; HUMAN CELL; MOLECULAR CLONING; PROMOTER REGION; SEQUENCE HOMOLOGY; SINGLE NUCLEOTIDE POLYMORPHISM; SOMATIC CELL; STOP CODON; BIOLOGICAL MODEL; CELL LINE; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENETIC PROCEDURES; GENETICS; METABOLISM; METHODOLOGY;

MAMMALIA;

BACTERIAL PROTEINS; CELL LINE; DNA; FLOW CYTOMETRY; FLUORESCENT DYES; GENE KNOCK-IN TECHNIQUES; GENE TARGETING; GENETIC TECHNIQUES; GENETIC VECTORS; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MODELS, GENETIC; PROMOTER REGIONS, GENETIC;

EID: 84857673053     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0032646     Document Type: Article

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