Identification of two residues essential for the stringent substrate specificity and active site stability of the prokaryotic l -arginine:glycine amidinotransferase CyrA

FEBS Journal

Volumn 279, Issue 5, 2012, Pages 805-815

  Muenchhoff, Julia ^a   Siddiqui, Khawar S ^a   Neilan, Brett A ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

amidinotransferase; cylindrospermopsin; enzyme kinetics; protein stability; site directed mutagenesis

Indexed keywords

BACTERIAL ENZYME; CYRA PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; NONHUMAN; PH; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; TEMPERATURE SENSITIVITY; WILD TYPE;

AMIDINOTRANSFERASES; ARGININE; BINDING SITES; CATALYSIS; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; CYLINDROSPERMOPSIS; HUMANS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN CONFORMATION; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY;

CYANOBACTERIA; CYLINDROSPERMOPSIS RACIBORSKII; PROKARYOTA;

EID: 84857641934     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2012.08472.x     Document Type: Article

References (22)

1. Carmichael WW, Azevedo SM, An JS, Molica RJ, Jochimsen EM, Lau S, Rinehart KL, Shaw GR, &, Eaglesham GK, (2001) Human fatalities from cyanobacteria: chemical and biological evidence for cyanotoxins. Environ Health Perspect 109, 663-668. (Pubitemid 32785421)
2. Bourke ATC, Hawes RB, Neilson A, &, Stallmann ND, (1983) An outbreak of hepato-enteritis (the Palm Island mystery disease) possibly caused by algal intoxication. Toxicon 21, 45-48.
3. Byth S, (1980) Palm Island mystery disease. Med J Aust 2, 40-42. (Pubitemid 11198179)
4. Ohtani I, Moore RE, &, Runnegar MTC, (1992) Cylindrospermopsin: a potent hepatotoxin from the blue-green alga Cylindrospermopsis raciborskii. J Am Chem Soc 114, 7941-7942.
5. Mihali TK, Kellmann R, Muenchhoff J, Barrow KD, &, Neilan BA, (2008) Characterization of the gene cluster responsible for cylindrospermopsin biosynthesis. Appl Environ Microbiol 74, 716-722. (Pubitemid 351225723)
6. Burgoyne DL, Hemscheidt TK, Moore RE, &, Runnegar MTC, (2000) Biosynthesis of cylindrospermopsin. J Org Chem 65, 152-156. (Pubitemid 30046368)
7. Muenchhoff J, Siddiqui KS, Poljak A, Raftery MJ, Barrow KD, &, Neilan BA, (2010) A novel prokaryotic L-arginine:glycine amidinotransferase is involved in cylindrospermopsin biosynthesis. FEBS J 277, 3844-3860.
8. Fritsche E, Humm A, &, Huber R, (1997) Substrate binding and catalysis by L-arginine:glycine amidinotransferase. Eur J Biochem 247, 483-490.
9. Humm A, Fritsche E, Mann K, Goehl M, &, Huber R, (1997) Recombinant expression and isolation of human L-arginine:glycine amidinotransferase and identification of its active site cysteine residue. Biochem J 322, 771-776.
10. Fritsche E, Humm A, &, Huber R, (1999) The ligand-induced structural changes of human L-arginine:glycine amidinotransferase. J Biol Chem 274, 3026-3032. (Pubitemid 29075390)
11. Poklar N, Lah J, Salobir M, Macek P, &, Vesnaver G, (1997) pH and temperature-induced molten globule-like denatured states of equinatoxin II: a study by UV-melting, DSC, far- and near-UV CD spectroscopy, and ANS fluorescence. Biochemistry 36, 14345-14352. (Pubitemid 27509926)
12. Siddiqui KS, Poljak A, De Francisci D, Guerriero G, Pilak O, Burg D, Raftery MJ, Parkin DM, Trewhella J, &, Cavicchioli R, (2010) A chemically modified alpha-amylase with a molten-globule state has entropically driven enhanced thermal stability. Protein Eng Des Sel 23, 769-780.
13. Georlette D, Damien B, Blaise V, Depiereux E, Uversky VN, Gerday C, &, Feller G, (2003) Structural and functional adaptations to extreme temperatures in psychrophilic, mesophilic, and thermophilic DNA ligases. J Biol Chem 278, 37015-37023. (Pubitemid 37175215)
14. Feller G, (2010) Protein stability and enzyme activity at extreme biological temperatures. J Phys Condens Matter 22, 323101-323118.
15. D'Amico S, Marx JC, Gerday C, &, Feller G, (2003) Activity-stability relationships in extremophilic enzymes. J Biol Chem 278, 7891-7896. (Pubitemid 36800524)
16. Fritsche E, Bergner A, Humm A, Piepersberg W, &, Huber R, (1998) Crystal structure of L-arginine:inosamine-phosphate amidinotansferase StrB1 from Streptomyces griseus: an enzyme involved in streptomycin biosynthesis. Biochemistry 37, 17664-17672. (Pubitemid 29023921)
17. Daniel RM, Danson MJ, &, Eisenthal R, (2001) The temperature optima of enzymes: a new perspective on an old phenomenon. Trends Biochem Sci 26, 223-225. (Pubitemid 32289239)
18. Siddiqui KS, &, Cavicchioli R, (2006) Cold-adapted enzymes. Annu Rev Biochem 75, 403-433. (Pubitemid 44118038)
19. Nohr J, &, Kristiansen K, (2003) Site-directed mutagenesis. Methods Mol Biol 232, 127-131.
20. Van Pilsum JF, Taylor D, Zakis B, &, McCormick P, (1970) Simplified assay for transamidinase activities of rat kidney homogenates. Anal Biochem 35, 277-286.
21. Walker JB, (1975) L-arginine:inosamine-phosphate amidinotransferase(s). Methods Enzymol 43, 451-458.
22. Lonhienne T, Gerday C, &, Feller G, (2000) Psychrophilic enzymes: revisiting the thermodynamic parameters of activation may explain local flexibility. Biochim Biophys Acta 1543, 1-10.