A comparison between micro-CT and histology for the evaluation of cortical bone: Effect of polymethylmethacrylate embedding on structural parameters

Journal of Microscopy

Volumn 245, Issue 3, 2012, Pages 302-310

  Particelli, F ^a   Mecozzi, L ^a   Beraudi, A ^a   Montesi, M ^a   Baruffaldi, F ^a   Viceconti, M ^a  

^a RIZZOLI ORTHOPAEDIC INSTITUTE   (Italy)

Author keywords

Cortical bone microstructure; Histology; Microcomputed tomography; PMMA embedding; Porosity; Thresholding

Indexed keywords

COMPUTERIZED TOMOGRAPHY; HISTOLOGY; HYDRAULIC STRUCTURES; MICROSTRUCTURE; PARAMETER ESTIMATION; POLYESTERS; POROSITY;

BONE MICROSTRUCTURE; CONDITION; CORTICAL BONE; CORTICAL BONE MICROSTRUCTURE; EMBEDDINGS; MICRO COMPUTED TOMOGRAPHY (MICRO-CT); PMMA EMBEDDING; STRUCTURAL PARAMETER; THRESHOLD-VALUE; THRESHOLDING;

BONE;

POLY(METHYL METHACRYLATE);

ARTICLE; BONE STRUCTURE; CONTROLLED STUDY; CORTICAL BONE; HAVERSIAN CANAL; HISTOLOGY; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; MICRO-COMPUTED TOMOGRAPHY; POROSITY; PRIORITY JOURNAL;

EID: 84857363911     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2011.03573.x     Document Type: Article

References (34)

1. Augat, P., Margevicius, K., Simon, J., Wolf, S., Suger, G. & Claes, L. (1998) Local tissue properties in bone healing: influence of size and stability of the osteotomy gap. J. Orthop. Res. 16, 475-481.
2. Basillais, A., Bensamoun, S., Chappard, C., Brunet-Imbault, B., Lemineur, G., Ilharreborde, B., Ho Ba Tho, M.C. & Benhamou, C.L. (2007) Three-dimensional characterization of cortical bone microstructure by microcomputed tomography: validation with ultrasonic and microscopic measurements. J. Orthop. Sci. 12, 141-148.
3. Bell, K.L., Loveridge, N., Jordan, G.R., Power, J., Constant, C.R. & Reeve, J. (2000) A novel mechanism for induction of increased cortical porosity in cases of intracapsular hip fracture. Bone 27, 297-304.
4. Beraudi, A., Stea, S., Bordini, B., Baleani, M. & Viceconti, M. (2010) Osteon classification in human fibular shaft by circularly polarized light. Cells Tissues Organs 191, 260-268.
5. Bousson, V., Peyrin, F., Bergot, C., Hausard, M., Sautet, A. & Laredo, J.D. (2004) Cortical bone in the human femoral neck: three-dimensional appearance and porosity using synchrotron radiation. J. Bone Miner. Res. 19, 794-801.
6. Britz, H.M., Jokihaara, J., Leppanen, O.V., Jarvinen, T. & Cooper, D.M. (2010) 3D visualization and quantification of rat cortical bone porosity using a desktop micro-CT system: a case study in the tibia. J. Microsc. 240, 32-37.
7. Cooper, D., Turinsky, A., Sensen, C. & Hallgrimsson, B. (2007) Effect of voxel size on 3D micro-CT analysis of cortical bone porosity. Calcif. Tissue Int. 80, 211-219.
8. Cooper, D.M., Matyas, J.R., Katzenberg, M.A. & Hallgrimsson, B. (2004) Comparison of microcomputed tomographic and microradiographic measurements of cortical bone porosity. Calcif. Tissue Int. 74, 437-447.
9. Cooper, D.M., Turinsky, A.L., Sensen, C.W. & Hallgrimsson, B. (2003) Quantitative 3D analysis of the canal network in cortical bone by micro-computed tomography. Anat. Rec. B. New. Anat. 274, 169-179.
10. Currey, J.D. (1988) The effect of porosity and mineral content on the Young's modulus of elasticity of compact bone. J. Biomech. 21, 131-139.
11. Feldkamp, L.A., Davis, L.C. & Kress, J.W. (1984) Practical cone-beam algorithm. J. Opt. Soc. Am. A. 1, 612-619.
12. Feldkamp, L.A., Goldstein, S.A., Parfitt, A.M., Jesion, G. & Kleerekoper, M. (1989) The direct examination of three-dimensional bone architecture in vitro by computed tomography. J. Bone Miner. Res. 4, 3-11.
13. Goulet, R.W., Goldstein, S.A., Ciarelli, M.J., Kuhn, J.L., Brown, M.B. & Feldkamp, L.A. (1994) The relationship between the structural and orthogonal compressive properties of trabecular bone. J. Biomech. 27, 375-389.
14. Hara, T., Tanck, E., Homminga, J. & Huiskes, R. (2002) The influence of microcomputed tomography threshold variations on the assessment of structural and mechanical trabecular bone properties. Bone 31, 107-109.
15. Hildebrand, T. & Ruegsegger, P. (1997) A new method for the model-independent assessment of thickness in three-dimensional images. J. Microsc. 185, 67.
16. Hubbell, J.H. (1996) Tables of X-Ray Mass Attenuation Coefficients and Mass Energy-Absorption Coefficients 1 keV to 20 MeV for Elements Z = 1 to 92 and 48 Additional Substances of Dosimetric Interest (ed. by S. M. Seltzer ). U.S. Department of Commerce, Technology Administration, National Institute of Standards and Technology, Gaithersburg, MD
17. Martin, R.B. & Ishida, J. (1989) The relative effects of collagen fiber orientation, porosity, density, and mineralization on bone strength. J. Biomech. 22, 419-426.
18. Matsumoto, T., Yoshino, M., Asano, T., Uesugi, K., Todoh, M. & Tanaka, M. (2006) Monochromatic synchrotron radiation muCT reveals disuse-mediated canal network rarefaction in cortical bone of growing rat tibiae. J. Appl. Physiol. 100, 274-280.
19. Muller, R., Van Campenhout, H., Van Damme, B., Van Der Perre, G., Dequeker, J., Hildebrand, T. & Ruegsegger, P. (1998) Morphometric analysis of human bone biopsies: a quantitative structural comparison of histological sections and micro-computed tomography. Bone 23, 59-66.
20. Parfitt, A.M., Drezner, M.K., Glorieux, F.H., Kanis, J.A., Malluche, H., Meunier, P.J., Ott, S.M. & Recker, R.R. (1987) Bone histomorphometry: standardization of nomenclature, symbols, and units. Report of the ASBMR Histomorphometry Nomenclature Committee. J. Bone Miner. Res. 2, 595-610.
21. Parkinson, I.H., Badiei, A. & Fazzalari, N.L. (2008) Variation in segmentation of bone from micro-CT imaging: implications for quantitative morphometric analysis. Australas Phys. Eng. Sci. Med. 31, 160-164.
22. Perilli, E., Baruffaldi, F., Visentin, M., Bordini, B., Traina, F., Cappello, A. & Viceconti, M. (2007) MicroCT examination of human bone specimens: effects of polymethylmethacrylate embedding on structural parameters. J. Microsc. 225, 192-200.
23. Sasov, A. & Van Dyck, D. (1998) Desktop X-ray microscopy and microtomography. J. Microsc. 191, 151-158.
24. Schaffler, M.B. & Burr, D.B. (1988) Stiffness of compact bone: effects of porosity and density. J. Biomech. 21, 13-16.
25. Schneider, P., Stauber, M., Voide, R., Stampanoni, M., Donahue, L.R. & Muller, R. (2007) Ultrastructural properties in cortical bone vary greatly in two inbred strains of mice as assessed by synchrotron light based micro- and nano-CT. J. Bone Miner. Res. 22, 1557-1570.
26. Spadaro, J.A., Werner, F.W., Brenner, R.A., Fortino, M.D., Fay, L.A. & Edwards, W.T. (1994) Cortical and trabecular bone contribute strength to the osteopenic distal radius. J. Orthop. Res. 12, 211-218.
27. Tassani, S., Ohman, C., Baruffaldi, F., Baleani, M. & Viceconti, M. (2011) Volume to density relation in adult human bone tissue. J. Biomech. 44, 103-108.
28. Thomsen, J.S., Laib, A., Koller, B., Prohaska, S., Mosekilde, L. & Gowin, W. (2005) Stereological measures of trabecular bone structure: comparison of 3D micro computed tomography with 2D histological sections in human proximal tibial bone biopsies. J. Microsc. 218, 171-179.
29. Waarsing, J.H., Day, J.S. & Weinans, H. (2004) An improved segmentation method for in vivo microCT imaging. J. Bone Miner. Res. 19, 1640-1650.
30. Wachter, N.J., Augat, P., Krischak, G.D., Mentzel, M., Kinzl, L. & Claes, L. (2001a) Prediction of cortical bone porosity in vitro by microcomputed tomography. Calcif. Tissue Int. 68, 38-42.
31. Wachter, N.J., Augat, P., Krischak, G.D., Sarkar, M.R., Mentzel, M., Kinzl, L. & Claes, L. (2001b) Prediction of strength of cortical bone in vitro by microcomputed tomography. Clin. Biomech. (Bristol, Avon) 16, 252-256.
32. Wachter, N.J., Krischak, G.D., Mentzel, M., Sarkar, M.R., Ebinger, T., Kinzl, L., Claes, L. & Augat, P. (2002) Correlation of bone mineral density with strength and microstructural parameters of cortical bone in vitro. Bone 31, 90-95.
33. Wang, X. & Ni, Q. (2003) Determination of cortical bone porosity and pore size distribution using a low field pulsed NMR approach. J. Orthop. Res. 21, 312-319.
34. Weibel, E.R. (1989) Measuring through the microscope: development and evolution of stereological methods. J. Microsc. 155, 393-403.