MicroCT examination of human bone specimens: Effects of polymethylmethacrylate embedding on structural parameters

Journal of Microscopy

Volumn 225, Issue 2, 2007, Pages 192-200

  Perilli, E ^a,b   Baruffaldi, F ^a   Visentin, M ^a   Bordini, B ^a   Traina, F ^a   Cappello, A ^b   Viceconti, M ^a  

^a RIZZOLI ORTHOPAEDIC INSTITUTE   (Italy)

^b UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Bone stereology; Embedding; MicroCT; Polymethylmethacrylate; Thresholding

Indexed keywords

EMBEDDINGS; PARAMETER ESTIMATION; VOLUME FRACTION;

BONE STEREOLOGY; BONE VOLUME FRACTION; EMBEDDINGS; MICRO CT; MICRO-TOMOGRAPHY; POLYMETHYLMETHACRYLATE; STEREOLOGY; STRUCTURAL PARAMETER; THRESHOLD-VALUE; THRESHOLDING;

POLYESTERS;

POLY(METHYL METHACRYLATE); SODIUM CHLORIDE;

ADULT; AGED; ARTICLE; BONE TISSUE; COMPARATIVE STUDY; CORTICAL BONE; FEMALE; HISTOLOGY; HUMAN; HUMAN TISSUE; MALE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; STRUCTURE ANALYSIS; TOMOGRAPHY; TRABECULAR BONE; X RAY ANALYSIS;

EID: 33847729554     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2007.01731.x     Document Type: Article

References (24)

1. Ding, M., Odgaard, A. & Hvid, I. (1999) Accuracy of cancellous bone volume fraction measured by micro-CT scanning. J. Biomech. 32, 323-326.
2. Dufresne, T. (1998) Segmentation techniques for analysis of bone by three-dimensional computed tomographic imaging. Technol. Health Care 6, 351-359.
3. Engelke, K., Graeff, W., Meiss, L., Hahn, M. & Delling, G. (1993) High spatial resolution imaging of bone mineral using computed microtomography. Comparison with microradiography and undecalcified histologic sections. Invest. Radiol. 28, 341-349.
4. Feldkamp, L.A., Davis, L.C. & Kress, J.W. (1984) Practical cone-beam algorithm. J. Optic. Soc. Am. A 1, 612-619.
5. Feldkamp, L.A., Goldstein, S.A., Parfitt, A.M., Jesion, G. & Kleerekoper, M. (1989) The direct examination of three-dimensional bone architecture in vitro by computed tomography. J. Bone Miner. Res. 4, 3-11.
6. Goulet, R.W., Goldstein, S.A., Ciarelli, M.J., Kuhn, J.L., Brown, M.B. & Feldkamp, L.A. (1994) The relationship between the structural and orthogonal compressive properties of trabecular bone. J. Biomech. 27, 375-389.
7. Hildebrand, T. & Rüegsegger, P. (1997a) A new method for the model-independent assessment of thickness in three-dimensional images. J. Microsc. 185, 67-75.
8. Hildebrand, T. & Rüegsegger, P. (1997b) Quantification of Bone Microarchitecture with the Structure Model Index. Comput. Methods Biomech. Biomed. Engin. 1, 15-23.
9. Hildebrand, T., Laib, A., Müller, R., Dequeker, J. & Rüegsegger, P. (1999) Direct three-dimensional morphometric analysis of human cancellous bone: microstructural data from spine, femur, iliac crest, and calcaneus. J. Bone Miner. Res. 14, 1167-1174.
10. Hubbel, J.H. & Seltzer, S.M. (1995) Tables of X-ray mass attenuation coefficients and mass energy-absorbtion coefficients. Gaithersburgh, MD, NIST, US Department of Commerce. NISTIR 5632.
11. Ito, M., Nakamura, T., Matsumoto, T., Tsurusaki, K. & Hayashi, K. (1998) Analysis of trabecular microarchitecture of human iliac bone using microcomputed tomography in patients with hip arthrosis with or without vertebral fracture. Bone 23, 163-169.
12. Kuhn, J.L., Goldstein, S.A., Feldkamp, L.A., Goulet, R.W. & Jesion, G.(1990) Evaluation of a microcomputed tomography system to study trabecular bone structure. J. Orthop. Res. 8, 833-842.
13. Müller, R. & Rüegsegger, P. (1997) Micro-tomographic imaging for the nondestructive evaluation of trabecular bone architecture. Stud. Health Technol. Inform. 40, 61-79.
14. Müller, R., Van Campenhout, H., Van Damme, B., Van Der Perre, G., Dequeker, J., Hildebrand, T. & Rüegsegger, P. (1998) Morphometric analysis of human bone biopsies: a quantitative structural comparison of histological sections and micro-computed tomography. Bone 23, 59-66.
15. Parfitt, A.M., Mathews, C.H., Villanueva, A.R., Kleerekoper, M., Frame, B. & Rao, D.S. (1983) Relationships between surface, volume, and thickness of iliac trabecular bone in aging and in osteoporosis. Implications for the microanatomic and cellular mechanisms of bone loss. J. Clin. Invest. 72, 1396-1409.
16. Parfitt, A.M., Drezner, M.K., Glorieux, F.H., Kanis, J.A., Malluche, H., Meunier, P.J., Ott, S.M. & Recker, R.R. (1987) Bone histomorphometry: standardization of nomenclature, symbols, and units. Report of the ASBMR Histomorphometry Nomenclature Committee. J. Bone Miner. Res. 2, 595-610.
17. Perilli, E., Baruffaldi, F., Bisi, M.C., Cristofolini, L. & Cappello, A. (2006) A physical phantom for the calibration of three-dimensional X-ray microtomography examination. J. Microsc. 222 (2), 124-134.
18. Rüegsegger, P., Koller, B. & Müller, R. (1996) A microtomographic system for the nondestructive evaluation of bone architecture. Calcif. Tissue Int. 58, 24-29.
19. Sasov, A. & Van Dyck, D. (1998) Desktop X-ray microscopy and microtomography. J. Microsc. 191 (2), 151-158.
20. Sasov, A.Y. (1987) Microtomography. Part 1: methods and equipment. J. Microsc. 147, 169-178.
21. Thomsen, J.S., Laib, A., Koller, B., Prohaska, S., Mosekilde, L. & Gowin, W. (2005) Stereological measures of trabecular bone structure: comparison of 3D micro computed tomography with 2D histological sections in human proximal tibial bone biopsies. J. Microsc. 218, 171-179.
22. van Ruijven, L.J., Giesen, E.B., Mulder, L., Farella, M. & van Eijden, T.M. (2005) The effect of bone loss on rod-like and plate-like trabeculae in the cancellous bone of the mandibular condyle. Bone 36, 1078-1085.
23. Waarsing, J.H., Day, J.S. & Weinans, H. (2004) An improved segmentation method for in vivo microCT imaging. J. Bone Miner. Res. 19, 1640-1650.
24. Weibel, E.R. (1980) Stereological Methods. Vol. 2: Theoretical Foundations. Academic Press, London.