Quantitative determination of the reduction of phototoxicity and photobleaching by controlled light exposure microscopy

Journal of Microscopy

Volumn 231, Issue 1, 2008, Pages 9-20

  Hoebe, R A ^a   Van Der Voort, H T M ^b   Stap, J ^a   Van Noorden, C J F ^a   Manders, E M M ^c  

^a ACADEMIC MEDICAL CENTER   (Netherlands)

^b Scientific Volume Imaging BV   (Netherlands)

^c UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

CLEM; Confocal microscopy; Fluorescence; Microscopy; Photobleaching; Phototoxicity

Indexed keywords

EXCITED STATES; FLUORESCENCE MICROSCOPY; FLUOROPHORES; IMAGE QUALITY; OPTICAL PROPERTIES; OXYGEN;

% REDUCTIONS; CONTROLED LIGHT EXPOSURE MICROSCOPY; EXCITED SINGLET STATE; EXCITED TRIPLET STATE; LIGHT EXPOSURE; LIVE CELL; PHOTO-TOXICITY; QUANTITATIVE DETERMINATIONS; REACTIVE OXYGEN SPECIES; SINGLET OXYGEN;

PHOTOBLEACHING;

ARTICLE; BLEACHING; CONTROLLED LIGHT EXPOSURE MICROSCOPY; ELECTRONICS; ILLUMINATION; IMAGE QUALITY; MEASUREMENT; MICROSCOPE; MICROSCOPY; OPTICAL RESOLUTION; PHOTOTOXICITY; PRIORITY JOURNAL;

EID: 46749142565     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2008.02009.x     Document Type: Article

References (17)

1. Bortner, C.D. & Cidlowski, J.A. (2002) Apoptotic volume decrease and the incredible shrinking cell. Cell Death Differ. 9, 1307 1310.
2. Centonze, V. & Pawley, J.B. (1995) Tutorial on practical confocal microscopy and use of the confocal test specimen. Handbook of Biological Confocal Microscopy (ed. by J.B. Pawley pp. 549 569. Plenum Press, New York.
3. Dixit, R. & Cyr, R. (2003) Cell damage and reactive oxygen species production induced by fluorescence microscopy: effect on mitosis and guidelines for non-invasive fluorescence microscopy. Plant J. 36, 280 290.
4. Eggeling, C., Widengren, J., Rigler, R. & Seidel, C.A.M. (1998) Photobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of two-step photolysis. Analyt. Chem. 70, 2651 2659.
5. Hoebe, R.A., Van Oven, C.H., Gadella, T.W. Jr., Dhonukshe, P.B., Van Noorden, C.J. & Manders, E.M. (2007) Controlled light-exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging. Nat. Biotechnol. 25, 249 253.
6. Kanda, T., Sullivan, K.F. & Wahl, G.M. (1998) Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr. Biol. 8, 377 385.
7. Manders, E.M. (2004) Method and apparatus for shaping an image of an object. Patent [WO2004102249]
8. Manders, E.M. (2005)Werkwijze en inrichting voor het vormen van een afbeelding van een object. Patent [NL1023440C].
9. McNally, J.G., Karpova, T., Cooper, J. & Conchello, J.A. (1999) Threedimensional imaging by deconvolution microscopy. Methods-A Comp Meth Enzymol 19, 373-385.
10. Shaw, P.J. & Rawlins, D.J. (1991) The point-spread function of a confocal microscope - its measurement and use in deconvolution of 3-D data. J. Microsc. 163, 151 165.
11. Shelby, R.D., Hahn, K.M. & Sullivan, K.F. (1996) Dynamic elastic behavior of alpha-satellite DNA domains visualized in situ in living human cells. J. Cell Biol 135, 545 557.
12. Stap, J., Krawczyk, P.M., Van Oven, C.H., Barendsen, G.W., Essers, J., Kanaar, R. & Aten, J.A. (2007) Induction of linear tracks of DNA double-strand breaks by alpha-particle irradiation of cells. Nat. Methods 5, 261 266.
13. Sugden, J.K. (2004) Photochemistry of dyes and fluorochromes used in biology and medicine: some physicochemical background and current applications. Biotech. Histochem. 79, 71 90.
14. Van Der Voort, H.T., Brakenhoff, G.J. & Baarslag, M.W. (1989) Three-dimensional visualization methods for confocal microscopy. J. Microsc. 153, 123 132.
15. Verveer, P.J. (1998) Computational and optical methods for improving resolution and signal quality in fluorescence microscopy. PhD Thesis, T. U. Delft, The Netherlands.
16. Wallace, W., Schaefer, L.H. & Swedlow, J.R. (2001) A working person's guide to deconvolution in light microscopy. Biotechniques 31, 1076 1097.
17. Wright, A., Bubb, W.A., Hawkins, C.L. & Davies, M.J. (2002) Singlet oxygen-mediated protein oxidation: evidence for the formation of reactive side chain peroxides on tyrosine residues. Photochem. Photobiol. 76, 35 46.