Separation and Purification of Polyhydroxyalkanoates from Newly Isolated Comamonas sp. EB172 by Simple Digestion with Sodium Hydroxide

Separation Science and Technology

Volumn 47, Issue 3, 2012, Pages 534-541

  Mohammadi, Mitra ^a   Hassan, Mohd Ali ^a   Shirai, Yoshihito ^b   Man, Hasfalina Che ^a   Ariffin, Hidayah ^a   Yee, Lian Ngit ^a   Mumtaz, Tabassum ^a   Chong, Mei Ling ^a   Phang, Lai Yee ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b KYUSHU INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Comamonas sp. EB172; Downstream processing; NaOH digestion; Polyhydroxyalkanoates; Purification; Recovery

Indexed keywords

ALKALINE SOLUTIONS; BACTERIAL CELLS; CELL WALLS; CELLULAR MATERIAL; COMAMONAS SP. EB172; DIGESTION TIME; DOWNSTREAM-PROCESSING; DRIED CELLS; LIPID LAYERS; NON-POLYMERIC; POLY-HYDROXYALKANOATE; POLYHYDROXYALKANOATES; POLYMER SUSPENSIONS; RECOVERY YIELD; SEPARATION AND PURIFICATION; SODIUM HYDROXIDE CONCENTRATION; SODIUM HYDROXIDES; WILD-TYPE BACTERIA;

BACTERIA; CELL MEMBRANES; ETHANOL; GLYCEROL; POLYMERS; PURIFICATION; RECOVERY; SODIUM;

MOLECULAR BIOLOGY;

EID: 84857245895     PISSN: 01496395     EISSN: 15205754     Source Type: Journal    
DOI: 10.1080/01496395.2011.615788     Document Type: Article

References (31)

1. Hassan, M.A, Shirai, Y. Kusubayashi, N. Karim, M.I.A. Nakanishi, K. Hashimoto, K. (1997) The production of polyhydroxyalkanoate from anaerobically treated palm oil mill effluent by Rhodobacter sphaeroides. J. Ferment. Bioeng., 83: 485.
2. Salehizadeh, H. Van Loosdrecht, M.C.M. (2004) Production of polyhydroxyalkanoates by mixed culture: Recent trends and biotechnological importance. Biotechnol. Adv., 22: 261.
3. Xu, Y. Wanga, R.H. Koutinas, A.A. Webba, C. (2010) Microbial biodegradable plastic production from a wheat-based biorefining strategy. Process. Biochem., 45: 153.
4. Mumtaz, T. Yahaya, N.A. Abd-Aziz, S. Abdul Rahman, N.A. Phang, L.Y. Shirai, Y. Hassan, M.A. (2010) Turning waste to wealth-biodegradable plastics polyhydroxyalkanoates from palm oil mill effluent - a Malaysian perspective. J. Cleaner Production, 18: 1393.
5. Zakaria, M.R. Abd-Aziz, S. Abdul Rahman, N.A. Yee, P.L. Shirai, Y. Hassan, M.A. (2008) Comamonas sp EB 172 isolated from anaerobic digester treating palm oil mill effluent as potential polyhydroxyalkanoate (PHA) producer. Afr. J. Biotechnol., 7: 4118.
6. Chisti, Y. Moo-Young, M. (1986) Disruption of microbial cells for intracellular products. J. Enzyme Microb. Technol., 8: 194.
7. Hopkins, T.R. (1991) Physical and chemical cell disruption for the recovery of intracellular proteins. J. Bioprocess. Technol., 12: 57.
8. Harrison, S.T.L. (1991) Bacterial cell disruption: A key unit operation in the recovery of intracellular products. J. Biotechnol. Adv., 9: 217.
9. Xuejun, C. (2006) A method for separating, extracting and purifying poly-beta-hydroxyalkaonates (PHAs) directly from bacterial fermentation broth. Patent Genius, Tianan Biolig Material Co Ltd [CN] EP1705250 (A1).
10. Choi, J.I. Lee, S.Y. (1999) Efficient and economical recovery of poly (3-hydroxy-butyrate) from recombinant Eshcherichia coli by simple digestion with chemicals. J. Biotechnol. Bioeng., 62: 546.
11. Lee, S.Y. Choi, J. Han, K. Song, J.Y. (1999) Removal of endotoxin during purification of poly(3-Hydroxybutyrate) from gram-negative bacteria. J. Appl. Environ. Microbiol., 65: 2762.
12. Jacquel, N. Lob, C.W. Wei, Y.H. Wu, H.S. Wang, S.S. (2008) Isolation and purification of bacterial poly(3-hydroxyalkanoates). Biochem. Eng. J., 39: 15.
13. Gorenflo, V. Schmack, G. Vogel, R. Steinbuchel, A. (2001) Development of a process for the biotechnological large-scale production of 4-hydroxyvalerate-containing polyesters and characterization of their physical and mechanical properties. Biomacromolecules., 2: 45.
14. Lu, C.H. (2006) Purification and separation of polyhydroxyalkanoates from bacteria [Ms Thesis]. Yuan Ze University, Taiwan.
15. Mumtaz, T. Abd-Aziz, S. Abdul Rahman, N.A. Phang, L.Y. Shirai, Y. Hassan, M.A. (2009) Fed-batch production of P(3HB-co-3HV) copolymer by Comamonas sp EB 172 using mixed organic acids under dual nutrient limitation. Eur. J. Sci. Res., 33: 374.
16. Bradford, M.M. (1976) A rapid and sensitive method for the quantization of microorganism quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248.
17. Braunegg, G. Sonnleitner, B. Lafferty, R.M. (1978) A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass. Eur. J. Appl. Microbiol. Biotechnol., 6: 29.
18. Ahmad, F.B.H. Ghaffari Moghaddam, M. Basri, M. Abdul Rahman, M.B. (2010) Anticancer activity of 3-O-acylated betulinic acid derivatives obtained by enzymatic synthesis. Biosci. Biotechnol. Biochem., 74: 1025.
19. Voon, P.T. (2005) Environmental friendly alternative methods for the recovery of intracellular polyhydroxyalkanoates (PHA) [Ms Thesis]. Universiti Putra Malaysia, Malaysia.
20. Jiang, X. Ramsay, J.A. Ramsay, B.A. (2006) Acetone extraction of mcl-PHA from Pseudomonas putida KT2440. J. Microbiol. Methods., 67: 212.
21. Yu, J. Plackett, D. Chen, L.X.L. (2005) Kinetics and mechanism of the monomeric products from abiotic hydrolysis of poly[(R)-3-hydroxybutyrate] under acidic and alkaline conditions. Polym. Degrad. Stabil., 89: 289.
22. Harrison, S.T. Dennis, J.S. Chase, H.A. (1991) Combined chemical and mechanical processes for the disruption of bacteria. J. Bioseparation, 2: 95.
23. Box, G.E. Hunter, W.G. Hunter, J.S. (2005) Statistics for Experimenters: Design, Innovation, and Discovery, 2nd Ed. Wiley, ISBN 0471718130.
24. Hejazi, P. Vasheghani-Farahani, E. Yamini, Y. (2003) Supercritical fluid disruption of Ralstonia eutropha for poly(β-hydroxybutyrate) recovery. Biotechnol. Prog., 19: 1519.
25. Khosravi-Darani, K. Vasheghani-Farahani, E. Shojaosadati, S.A. Yamini, Y. (2004) Effect of process variables on supercritical fluid disruption of Ralstonia eutropha cells for poly(R-hydroxybutyrate) recovery. Biotechnol. Prog., 20: 1757.
26. Kapritchkoff, F.M. Viotti, A.P. Alli, R.C.P. Zuccolo, M. Pradella, J.G.C. Maiorano, A.E. Miranda, E.A. Bonomi, A. (2006) Enzymatic recovery and purification of polyhydroxybutyrate produced by Ralstonia eutropha. J. Biotechnol., 122: 453.
27. Divyashree, M.S. Shamala, T.R. Rastogi, N.K. (2009) Isolation of polyhydroxyalkanoate from hydrolyzed cells of Bacillus flexus using aqueous two-phase system containing polyethylene glycol and phosphate. Biotechnol. Bioprocess. Eng., 14: 482.
28. Divyashree, M.S. Shamala, T.R. (2010) Extractability of polyhydroxyalkanoate synthesized by Bacillus flexus cultivated in organic and inorganic nutrient media. Indian J. Microbiol., 50: 63.
29. Page, W.J. Cornish, A. (1993) Growth of Azotobacter vinelandii UWD in fish peptone medium and simplified extraction of poly-b-hydroxybutyrate. Appl. Environ. Microbiol., 59: 4236.
30. Gottlieb, H.E. Kotlyar, V. Nudelman, A. (1997) NMR chemical shifts of common laboratory solvents as trace impurities. J. Org. Chem., 62: 7512.
31. Yanagita, Y. Ogawa, N. Ueda, Y. Osakada, F. Matsumoto, K. (2008) Method of collecting highly pure polyhydroxyalkanoate from microbial cells. United States Kaneka Corporation (Osaka-shi, JP) 7393668.