PCR product detector with LED-photodiode fluorescence sensing in a nanoliter flow-cell for the high-throughput detection of double-stranded DNA

Procedia Engineering

Volumn 25, Issue , 2011, Pages 936-939

  Peham, Johannes R ^a   Vellekoop, Michael J ^b   Nohammer, Christa ^a   Wiesinger Mayr, Herbert ^a  

^a AIT AUSTRIAN INSTITUTE OF TECHNOLOGY   (Austria)

^b VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Detection; DNA; Flow cell; High throughput; LED; Lens free; PCR; Photodiode; Sensor; SYBR Green

Indexed keywords

FLOW CELL; HIGH THROUGHPUT; LENS-FREE; PCR; SYBR GREEN;

DNA; ERROR DETECTION; FLUORESCENCE; LIGHT EMITTING DIODES; PHOTODIODES; SENSORS;

POLYMERASE CHAIN REACTION;

EID: 84857162760     PISSN: 18777058     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1016/j.proeng.2011.12.230     Document Type: Conference Paper

References (3)

1. Peham JR, Grienauer W, Sterner H, Heer R, Vellekoop MJ, Noehammer C, Wiesinger-Mayr H. Long target droplet polymerase chain reaction with a microfluidic device for high-throughput detection of pathogenic bacteria at clinical sensitivity. Biomed Microdevices 2011; 13(3):463-473.
2. Manage DP, Morrissey YC, Stickel AJ, Lauzon J, Atrazhev A, Acker JP, Pilarski LM. On-chip PCR amplification of genonmic and viral templates in unprocessed whole blood. Microfluid Nanofluid 2011; 10(3):697-702.
3. Hopwood AJ, Hurth C, Yang J, Cai Z, Moran N, Lee-Edghill JG, Nordquist A, Lengik R, Estes MD, Haley JP, McAlister CR, Chen X, Brooks C, Smith S, Elhott K, Kuomi P, Zenhausern F, Tully G. Integrated Microfluidic System for Rapid Forensic DNA Analysis: Sample Collection to DNA Profile. Anal Chem 2010; 82:6991-6999.