Long target droplet polymerase chain reaction with a microfluidic device for high-throughput detection of pathogenic bacteria at clinical sensitivity

Biomedical Microdevices

Volumn 13, Issue 3, 2011, Pages 463-473

  Peham, Johannes R ^a   Grienauer, Walter ^b   Steiner, Hannes ^b   Heer, Rudolf ^a   Vellekoop, Michael J ^c   Nöhammer, Christa ^a   Wiesinger Mayr, Herbert ^a  

^a AIT AUSTRIAN INSTITUTE OF TECHNOLOGY   (Austria)

^b Aerospace and Advanced Composites GmbH   (Austria)

^c VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Bacteria; High throughput; Long target; Microfluidic; Pathogen detection; Polymerase chain reaction

Indexed keywords

16S RIBOSOMAL RNA; AMPLICONS; ANTIBIOTIC TREATMENT; BACTERIAL CELLS; CLINICAL APPLICATION; CONTINUOUS MODE; DETECTION LIMITS; DNA CONCENTRATION; DNA MICRO-ARRAY; EARLY DIAGNOSIS; GRAM-NEGATIVE PATHOGENS; HEATING PLATES; HIGH THROUGHPUT; HIGH-THROUGHPUT DETECTION; INFECTIOUS DISEASE; LONG TARGET; MAXIMAL THROUGHPUT; MICRO-FLUIDIC DEVICES; MICROARRAY TECHNOLOGIES; PATHOGEN DETECTION; PATHOGENIC BACTERIUM; PCR EFFICIENCY; PSEUDOMONAS AERUGINOSA; SAMPLE NUMBER; SINGLE REACTOR; SPECIES CLASSIFICATION; SPECIES IDENTIFICATION; SPEED-UPS; STAPHYLOCOCCUS AUREUS; TARGET SEQUENCES; TOTAL PROCESSING TIME; TRIANGULAR PRISM;

BACTERIA; BACTERIOLOGY; BIOASSAY; BIOCHIPS; COILED TUBING; DIAGNOSIS; DNA; DNA SEQUENCES; DROPS; ESCHERICHIA COLI; FLUIDIC DEVICES; FLUORINE CONTAINING POLYMERS; GENES; MICROFLUIDICS; PATHOGENS; POLYMERIZATION; POLYMERS; PRISMS; RNA; THROUGHPUT;

POLYMERASE CHAIN REACTION;

BACTERIAL DNA; BACTERIAL RNA; DNA FRAGMENT; RNA 16S;

ARTICLE; CONTROLLED STUDY; DNA ISOLATION; DNA MICROARRAY; DNA SEQUENCE; ESCHERICHIA COLI; GENE AMPLIFICATION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HIGH THROUGHPUT SCREENING; MICROFLUIDICS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; QUALITATIVE ANALYSIS; QUALITY CONTROL; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE; SPECIES IDENTIFICATION; STAPHYLOCOCCUS AUREUS;

ANIMALS; BACTERIA; CATTLE; HUMANS; LIMIT OF DETECTION; MICROFLUIDIC ANALYTICAL TECHNIQUES; POLYMERASE CHAIN REACTION; QUALITY CONTROL; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; TIME FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS;

EID: 79958126754     PISSN: 13872176     EISSN: 15728781     Source Type: Journal    
DOI: 10.1007/s10544-011-9514-x     Document Type: Article

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