The active site residue V266 of chlamydial HtrA is critical for substrate binding during both in vitro and in vivo conditions

Journal of Molecular Microbiology and Biotechnology

Volumn 22, Issue 1, 2012, Pages 10-16

  Gloeckl, Sarina ^a   Tyndall, Joel D A ^b   Stansfield, Scott H ^a   Timms, Peter ^a   Huston, Wilhelmina M ^a  

^a QUEENSLAND UNIVERSITY OF TECHNOLOGY   (Australia)

^b UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

Active site binding specificity; Bacterial adenylate cyclase two hybrid; Chlamydia; HtrA; Protease

Indexed keywords

BACTERIAL PROTEIN; CHLAMYDIA TRACHOMATIS HTRA PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR MODEL; PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS; TWO HYBRID SYSTEM; WILD TYPE;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CATALYTIC DOMAIN; CHLAMYDIA TRACHOMATIS; MODELS, MOLECULAR; MOLECULAR CHAPERONES; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; SERINE PROTEASES; TWO-HYBRID SYSTEM TECHNIQUES; VIRULENCE FACTORS;

BACTERIA (MICROORGANISMS); CHLAMYDIA; CHLAMYDIA TRACHOMATIS;

EID: 84857136796     PISSN: 14641801     EISSN: 16602412     Source Type: Journal    
DOI: 10.1159/000336312     Document Type: Article

References (20)

1. Baud C, Gutsche I, Willery E, de Paepe D, Derobecq H, Gilleron M, Locht C, Jamin M, Jacob-Dubuisson F: Membrane-associated DegP in Bordetella chaperones a repeat-rich secretory protein. Mol Microbiol 2011; 80: 1625-1635.
2. Belland RJ, Nelson DE, Virokl D, Crane DD, Hogan D, Sturdevant D, Beatty WL, Caldwell HD: Transcriptome analysis of chlamydial growth during IFN-γ-mediated persistence and reactivation. Proc Natl Acad Sci USA 2003; 100: 15971-15976. (Pubitemid 38021099)
3. Benjamini Y, Hochberg Y: Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Statist Soc Ser 1995; 57: 289-300.
4. Chitlaru T, Zaide G, Ehrlich SD, Inbar I, Cohen O, Shafferman A: HtrA is a major virulence determinant of Bacillus anthracis . Mol Microbiol 2011;81: 1542-1559.
5. Clarke L, Carbon J: A colony bank containing synthetic ColE1 hybrid plasmids representative of the entire E. coli chromosome. Cell 1976; 9: 91-99.
6. Garnier J, Gibrat JF, Robson B: GOR method for predicting protein secondary structure from amino acid sequence. Methods Enzymol 1996; 266: 540-553.
7. Ghinsberg RC, Nitzan Y: Chlamydia trachomatis direct isolation, antibody prevalence and clinical symptoms in women attending outpatient clinics. New Microbiol 1994; 17: 231-242.
8. Hoy B, Lower M, Weydig C, Carra G, Tegtmeyer N, Geppert T, Schroder P, Sewald N, Backert S, Schneider G, Wessler S: Helicobacter pylori HtrA is a new secreted virulence factor that cleaves E-cadherin to disrupt intercellular adhesion. EMBO Rep 2010; 11: 798-804.
9. Huston WM, Swedberg JE, Harris JM, Walsh TP, Mathews SA, Timms P: The temperature-activated HtrA protease from pathogen Chlamydia trachomatis acts as both a chaperone and protease at 37 ° C. FEBS Lett 2007; 581: 3382-3386. (Pubitemid 47048181)
10. Huston WM, Tyndall JD, Lott WB, Stansfield SH, Timms P: Unique residues involved in activation of the multitasking protease/chaperone HtrA from Chlamydia trachomatis . PLoS One 2011; 6: e24547.
11. Jones CH, Dexter P, Evans AK, Liu C, Hultgren SJ, Hruby DE: Escherichia coli DegP protease cleaves between paired hydrophobic residues in a natural substrate: the PapA pilin. J Bacteriol 2002; 184: 5762-5771.
12. Krojer T, Garrido-Franco M, Huber R, Ehrmann M, Clausen T: Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine. Nature 2002; 416: 455-459. (Pubitemid 34272883)
13. Krojer T, Pangerl K, Kurt J, Sawa J, Stingl C, Mechtler K, Huber R, Ehrmann M, Clausen T: Interplay of PDZ and protease domain of DegP ensures efficient elimination of misfolded proteins. Proc Natl Acad Sci USA 2008; 105: 7702.
14. Krojer T, Sawa J, Huber R, Clausen V: HtrA proteases have a conserved activation mechanism that can be triggered by distinct molecular cues. Nat Struct Mol Biol 2010; 17: 844-852.
15. Krojer T, Sawa J, Schäfer E, Saibil HR, Ehrmann M, Clausen T: Structural basis for the regulated protease and chaperone function of DegP. Nature 2008; 453: 885-890. (Pubitemid 351832567)
16. Poquet I, Saint V, Seznec E, Simoes N, Bolotin A, Gruss A: HtrA is the unique surface housekeeping protease in Lactococcus lactis and is required for natural protein processing. Mol Microbiol 2000; 35: 1042-1051. (Pubitemid 30137774)
17. Sobiecka-Szkatula A, Gieldon A, Scire A, Tanfani F, Figaj D, Koper T, Ciarkowski J, Lipinska B, Skorko-Glonek J: The role of the L2 loop in the regulation and maintaining the proteolytic activity of HtrA (DegP) protein from Escherichia coli . Arch Biochem Biophys 2010; 500: 123-130.
18. Sontag D, Singh R, Berger B: Probabilistic modeling of systematic errors in two-hybrid experiments. Pac Symp Biocomput 2007; 1: 445-457.
19. Witkin SS, Linhares IM: Chlamydia trachomatis in subfertile women undergoing uterine instrumentation-an alternative to direct microbial testing or prophylactic antibiotic treatment. Hum Reprod 2002; 17: 1938-1941.
20. World Health Organisation, 2011: http://www. who.int/vaccine-research/ diseases/soa-std/ed/index1/html.