A simple technique for early in vivo electroporation of E1 chick embryos

Developmental Dynamics

Volumn 241, Issue 3, 2012, Pages 545-552

  Brown, Charmaine Y ^a   Seok Eom, Dae ^a,c   Amarnath, Smita ^b   Agarwala, Seema ^a,b  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b Section of Molecular Cell and Developmental Biology   (United States)

^c UNIVERSITY OF WASHINGTON   (United States)

Author keywords

E1; Gene manipulations; Gene misexpression; In ovo electroporation; Neural plate

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL PROLIFERATION; CELL STRUCTURE; CELL SURVIVAL; CHICK; CONTROLLED STUDY; DEVELOPMENTAL STAGE; ELECTROPORATION; EMBRYO DEVELOPMENT; GENE FUNCTION; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; MESENCEPHALON; MITOSIS; NEURAL TUBE DEFECT; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ANALYSIS; THREE DIMENSIONAL IMAGING;

ANIMALS; CELL PROLIFERATION; CHICK EMBRYO; ELECTROPORATION; GENE TRANSFER TECHNIQUES; MESENCEPHALON; ORGAN SIZE;

SPONDIAS PURPUREA;

EID: 84857046935     PISSN: 10588388     EISSN: 10970177     Source Type: Journal    
DOI: 10.1002/dvdy.23747     Document Type: Article

References (17)

1. Agarwala S, Sanders TA, Ragsdale CW. 2001. Sonic hedgehog control of size and shape in midbrain pattern formation. Science 291: 2147-2150.
2. Bayly RD, Ngo M, Aglyamova GV, Agarwala S. 2007. Regulation of ventral midbrain patterning by Hedgehog signaling. Development 134: 2115-21124.
3. Eom D, Amarnath S, Fogel JL, Agarwala S. 2011. Bone morphogenetic proteins regulate neural tube closure by interacting with the apicobasal polarity pathway. Development 138: 3179-3188.
4. Funahashi J-i, Okafuji T, Ohuchi H, Noji S, Tanaka H, Nakamura H. 1999. Role of Pax-D in the regulation of a mid-hindbrain organizer's activity. Dev Growth Differ 41: 59-72.
5. Gammill LS, Krull CE. 2011. Embryological and genetic manipulation of chick development. In: Pelegri, FJ, editor. Vertebrate embryogenesis, Methods. Mol. Biol. Dordrecht: Springer Science. 770: 119-137.
6. Hamburger V, Hamilton HL. 1951. A series of normal tages in the development of the chick embryo. Journal of Morphology 88: 49-92.
7. Hatakeyama J, Shimamura K. 2008. Method for electroporation for the early chick embryo. Dev Growth Differ 50: 449-452.
8. Hong E, Brewster R. 2006. N-cadherin is required for the polarized cell behaviors that drive neurulation in the zebrafish. Development 133: 3895-9905.
9. Itasaki N, Bel-Vialar S, Krumlauf R. 1999. "Shocking" developments in chick embryology: electroporation and in ovo gene expression. Nature Cell Biol 1: 203-207.
10. Johnson AD, Krieg PA. 1994. pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos. Gene 147: 223-226.
11. Momose T, Tonegawa A, Takeuchi J, Ogawa H, Umesono K, Yasuda K. 1999. Efficient targeting of gene expression in chick embryos by microelectroporation. Dev Growth Differ 41: 335-344.
12. Muramatsu T, Mizutani Y, Ohmori Y, Okumura J-i. 1997. Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo. Biochem Biophys Res Commun 230: 376-380.
13. Sakamoto K, Nakamura H, Takagi M, Takeda S, Katsube K-i. 1998. Ectopic expression of lunatic Fringe leads to downregulation of Serrate-1 in the developing chick neural tube; analysis using in ovo electroportation transfection technique. FEBS Lett 426: 337-341.
14. Sauka-Spengler T, Barembaum M. 2008. Gain- and loss-of-function approaches in the chick embryo. Methods Cell Biol 87: 237-256.
15. Tanaka J, Harada H, Ito K, Ogura T, Nakamura H. 2010. Gene manipulation of chick embryos in vitro, early chick culture, and long survival in transplanted eggs. Dev Growth Differ 52: 629-634.
16. Uchikawa M. 2008. Enhancer analysis by chicken embryo electroporation with the aid of genome comparison. Dev Growth Differ 50: 467-474.
17. Voiculescu O, Papanayotou C, Stern CD. 2008. Spatially and temporally controlled electroporation of early chick embryos. Nature Protocols 3: 419-426.