Intravital imaging of the mouse thymus using 2-photon microscopy

Journal of Visualized Experiments

Volumn , Issue 59, 2012, Pages 1-5

  Caetano, Susana S ^a   Teixeira, Tatiana ^a   Tadokoro, Carlos E ^a  

^a INSTITUTO GULBENKIAN DE CIÊNCIA   (Portugal)

Author keywords

2 photon; Immunology; In vivo; Intravital; Issue 59; Regulatory T cells; Thymus

Indexed keywords

EID: 84857007773     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3504     Document Type: Article

References (12)

1. Bettelli, E., et al. Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cells. Nature. 441, 235-238 (2006).
2. Ramsdell, F., Zúñiga-Pflücker, J.C., & Takahama, Y. In vitro systems for the study of T cell development: fetal thymus organ culture and OP9-DL1 cell coculture. Current protocols in immunology edited by John E. Coligan... [et al.] Chapter 3, Unit 3.18 (2006).
3. Tadokoro, C.E., et al. Regulatory T cells inhibit stable contacts between CD4+ T cells and dendritic cells in vivo. J. Exp. Med. 203, 505-511 (2006).
4. Mempel, T.R., Henrickson, S.E., & Von Andrian, U.H. T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases. Nature. 427, 154-159 (2004).
5. Miller, M.J., et al. Imaging the single cell dynamics of CD4+ T cell activation by dendritic cells in lymph nodes. J. Exp. Med. 200, 847-856 (2004).
6. Shakhar, G., et al. Stable T cell-dendritic cell interactions precede the development of both tolerance and immunity in vivo. Nat. Immunol. 6, 707-714 (2005).
7. Hugues, S., et al. Distinct T cell dynamics in lymph nodes during the induction of tolerance and immunity. Nat. Immunol. 5, 1235-1242 (2004).
8. Tang, Q., et al. Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice. Nat. Immunol. 7, 83-92 (2006).
9. Le Borgne, M. et al. The impact of negative selection on thymocyte migration in the medulla. Nature immunology. 10, 823-30 (2009).
10. Baez, S. An open cremaster muscle preparation for the study of blood vessels by in vivo microscopy. Microvascular research. 5, 384-94 (1973).
11. Wang, B., Zinselmeyer, B.H., McDole, J.R., Gieselman, P.A., & Miller, M.J. Non-invasive Imaging of Leukocyte Homing and Migration in vivo. J. Vis. Exp. (46), e2062, DOI: 10.3791/2062 (2010).
12. Barretto, R.P.J., et al. Time-lapse imaging of disease progression in deep brain areas using fluorescence microendoscopy. Nature medicine. 17, 223-228 (2011).