Preparation of intact bovine tail intervertebral discs for organ culture

Journal of Visualized Experiments

Volumn , Issue 60, 2012, Pages 1-7

  Chan, Samantha C W ^a   Gantenbein Ritter, Benjamin ^a  

^a UNIVERSITY OF BERN   (Switzerland)

Author keywords

Bioengineering; Bioreactor; Cartilaginous endplates; Cell viability; Diffusion; Growth plate; Intervertebral disc; Issue 60; Mechanical loading; Nutrition; Organ culture; Tissue engineering

Indexed keywords

EID: 84856988419     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3490     Document Type: Article

References (11)

1. Lee, C.R., Iatridis, J.C., Poveda, L., & Alini, M. In vitro organ culture of the bovine intervertebral disc: effects of vertebral endplate and potential for mechanobiology studies. Spine (Phila Pa 1976). 31, 515-522 (2006).
2. Chan, S.C.W., Gantenbein-Ritter, B., Leung, V.Y., Chan, D., et al. Cryopreserved intervertebral disc with injected bone marrow-derived stromal cells: a feasibility study using organ culture. Spine. J. 10 (6), 486-496 (2010).
3. Chan, S.C., Ferguson, S.J., Wuertz, K., & Gantenbein-Ritter, B. Biological Response of the Intervertebral Disc to Repetitive Short Term Cyclic Torsion. Spine (Phila Pa 1976). (2011).
4. Gantenbein-Ritter, B., Sprecher, C.M., Chan, S., Illien-Jünger, S., & Grad, S. Confocal imaging protocols for live/dead staining in three-dimensional carriers. Methods Mol. Biol. 740, 127-140 (2011).
5. Gantenbein-Ritter, B., Potier, E., Zeiter, S., van der Werf, M., et al. Accuracy of three techniques to determine cell viability in 3D tissues or scaffolds. Tissue Engineering Part C Methods. 14, 353-358 (2008).
6. Rasband, W.S., Image J, National Institutes of Health, Bethesda, Maryland, USA, http://rsb.info.nih.gov/ij/, 1997-2007 (2007).
7. Haschtmann, D., Stoyanov, J.V., Ettinger, L., Nolte, L.P., & Ferguson, S.J. Establishment of a novel intervertebral disc/endplate culture model: analysis of an ex vivo in vitro whole-organ rabbit culture system. Spine. 31, 2918-2925 (2006).
8. Gantenbein, B., Grünhagen, T., Lee, C.R., van Donkelaar, C.C., et al. An in vitro organ culturing system for intervertebral disc explants with vertebral endplates: a feasibility study with ovine caudal discs. Spine. 31, 2665-2673 (2006).
9. Korecki, C.L., Kuo, C.K., Tuan, R.S., & Iatridis, J.C. Intervertebral disc cell response to dynamic compression is age and frequency dependent. J. Orthop. Res. 27, 800-806 (2009).
10. Korecki, C.L., MacLean, J.J., & Iatridis, J.C. Dynamic compression effects on intervertebral disc mechanics and biology. Spine (Phila Pa 1976). 33, 1403-1409 (2008).
11. Jim, B., Steffen, T., Moir, J., Roughley, P., & Haglund, L. Development of an intact intervertebral disc organ culture system in which degeneration can be induced as a prelude to studying repair potential. European Spine Journal., 1-11 (2011).