Measuring peptide translocation into large unilamellar vesicles

Journal of Visualized Experiments

Volumn , Issue 59, 2012, Pages 1-5

  Spinella, Sara A ^a   Nelson, Rachel B ^a   Elmore, Donald E ^a  

^a WELLESLEY COLLEGE   (United States)

Author keywords

Fret; Issue 59; Membrane translocation; Molecular biology; Peptide; Tryptophan; Vesicle

Indexed keywords

EID: 84856978976     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3571     Document Type: Article

References (22)

1. Henriques, S.T., Melo, M.N., & Castanho, M.A. Cell-penetrating peptides and antimicrobial peptides: how different are they? Biochem. J. 399 (1), 1 (2006).
2. Trehin, R. & Merkle, H.P. Chances and pitfalls of cell penetrating peptides for cellular drug delivery. Eur. J. Pharm. Biopharm. 58 (2), 209 (2004).
3. Temsamani, J. & Vidal, P. The use of cell-penetrating peptides for drug delivery. Drug Discov. Today. 9 (23), 1012 (2004).
4. Hale, J.D. & Hancock, R.E. Alternative mechanisms of action of cationic antimicrobial peptides on bacteria. Expert Review of Anti-Infective Therapy. 5 (6), 951 (2007).
5. Nicolas, P. & Rosenstein, Y. Multifunctional host defense peptides. FEBS J. 276 (22), 6464 (2009).
6. Boman, H.G., Agerberth, B., & Boman, A. Mechanisms of Action on Escherichia-Coli of Cecropin-P1 and Pr-39, 2 Antibacterial Peptides from Pig Intestine. Infection and Immunity. 61 (7), 2978 (1993).
7. Park, C.B., Kim, H.S., & Kim, S.C. Mechanism of action of the antimicrobial peptide buforin II: Buforin II kills microorganisms by penetrating the cell membrane and inhibiting cellular functions. Biochemical and Biophysical Research Communications. 244 (1), 253 (1998).
8. Bobone, S., et al. The thin line between cell-penetrating and antimicrobial peptides: the case of Pep-1 and Pep-1-K. J. Pept. Sci. 17 (5), 335 (2011).
9. Marks, J.R., Placone, J., Hristova, K., & Wimley, W.C. Spontaneous Membrane-Translocating Peptides by Orthogonal High-throughput Screening. J. Am. Chem. Soc. (2011).
10. Rosenbluh, J., et al. Translocation of histone proteins across lipid bilayers and Mycoplasma membranes. J. Mol. Biol. 345 (2), 387 (2005).
11. Bárány-Wallje, E., et al. A critical reassessment of penetratin translocation across lipid membranes. Biophys. J. 89 (4), 2513 (2005).
12. Henriques, S.T., Costa, J., & Castanho, M.A. Translocation of beta-galactosidase mediated by the cell-penetrating peptide pep-1 into lipid vesicles and human HeLa cells is driven by membrane electrostatic potential. Biochemistry. 44 (30), 10189 (2005).
13. Orioni, B., et al. Membrane perturbation by the antimicrobial peptide PMAP-23: a fluorescence and molecular dynamics study. Biochim. Biophys. Acta. 1788 (7), 1523 (2009).
14. Ladokhin, A.S., Jayasinghe, S., & White, S.H. How to measure and analyze tryptophan fluorescence in membranes properly, and why bother? Anal. Biochem. 285 (2), 235 (2000).
15. Epand, R.M. & Epand, R.F. Liposomes as models for antimicrobial peptides. Methods Enzymol. 372, 124 (2003).
16. Kobayashi, S., et al. Interactions of the novel antimicrobial peptide buforin 2 with lipid bilayers: Proline as a translocation promoting factor. Biochemistry. 39 (29), 8648 (2000).
17. Matsuzaki, K., Murase, O., Fujii, N., & Miyajima, K. Translocation of a channel-forming antimicrobial peptide, magainin 2, across lipid bilayers by forming a pore. Biochemistry. 34 (19), 6521 (1995).
18. Henriques, S.T., Costa, J., & Castanho, M.A. Re-evaluating the role of strongly charged sequences in amphipathic cell-penetrating peptides: a fluorescence study using Pep-1. FEBS Lett. 579 (20), 4498 (2005).
19. Torchilin, V.P. & Weissig, V. Liposomes, 2nd ed., Oxford University Press, New York, (2003).
20. Avanti Polar Lipids, Determination of Total Phosphorus, http://www.avantilipids.com/index.php?option=com_content&view=article&id=1686&Itemid405
21. Almeida, P.F. & Pokorny, A. Mechanisms of antimicrobial, cytolytic, and cell-penetrating peptides: from kinetics to thermodynamics. Biochemistry. 48 (34), 8083 (2009).
22. Kobayashi, S., et al. Membrane translocation mechanism of the antimicrobial peptide buforin 2. Biochemistry. 43 (49), 15610 (2004).