Comparison of six different specimen types for Epstein-Barr viral load quantification in peripheral blood of pediatric patients after heart transplantation or after allogeneic hematopoietic stem cell transplantation

Journal of Clinical Virology

Volumn 53, Issue 3, 2012, Pages 186-194

  Ruf, S ^a   Behnke Hall, K ^a   Gruhn, B ^b   Bauer, J ^a   Horn, M ^a   Beck, J ^b   Reiter, A ^a   Wagner, H J ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b JENA UNIVERSITY HOSPITAL   (Germany)

Author keywords

Epstein Barr Virus; Hematopoietic stem cell transplantation; Post transplant lymphoproliferative disorder; Solid organ transplantation; Viral load

Indexed keywords

RITUXIMAB; VIRUS DNA;

ADOLESCENT; ADULT; ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION; ARTICLE; B LYMPHOCYTE; BLOOD ANALYSIS; CHILD; CONTROLLED STUDY; CORRELATIONAL STUDY; EPSTEIN BARR VIRUS; FEMALE; HEART TRANSPLANTATION; HUMAN; INFANT; LYMPHOPROLIFERATIVE DISEASE; MAJOR CLINICAL STUDY; MALE; PEDIATRICS; PERIPHERAL BLOOD MONONUCLEAR CELL; POSTTRANSPLANT LYMPHOPROLIFERATIVE DISORDER; PRESCHOOL CHILD; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RISK ASSESSMENT; SCHOOL CHILD; SENSITIVITY AND SPECIFICITY; SPECIES COMPARISON; VIRUS DETECTION; VIRUS LOAD;

ADOLESCENT; ADULT; B-LYMPHOCYTES; CHILD; CHILD, PRESCHOOL; COHORT STUDIES; DNA, VIRAL; EPSTEIN-BARR VIRUS INFECTIONS; HEART TRANSPLANTATION; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HERPESVIRUS 4, HUMAN; HUMANS; INFANT; LEUKOCYTES, MONONUCLEAR; LYMPHOPROLIFERATIVE DISORDERS; REFERENCE VALUES; SENSITIVITY AND SPECIFICITY; STATISTICS, NONPARAMETRIC; TRANSPLANTATION, HOMOLOGOUS; VIRAL LOAD;

EID: 84856952788     PISSN: 13866532     EISSN: 18735967     Source Type: Journal    
DOI: 10.1016/j.jcv.2011.11.010     Document Type: Article

References (44)

1. Young L.S., Rickinson A.B. Epstein-Barr virus: 40 years on. Nat Rev Cancer 2004, 4:757-768.
2. Stevens S.J.C., Pronk I., Middeldorp J.M. Toward standardization of Epstein-Barr Virus DNA load monitoring: unfractionated whole blood as preferred clinical specimen. J Clin Microbiol 2001, 39:1211-1216.
3. van Esser W.J.W., Niesters H.G.M., van der Holt B., Meijer E., Osterhaus A.D.M.E., Gratama J.W., et al. Prevention of Epstein-Barr virus-lymphoproliferative disease by molecular monitoring and preemptive rituximab in high-risk patients after allogeneic stem cell transplantation. Blood 2002, 99:4364-4369.
4. Holmes H., Davis C., Heath A., Hewlett I., Lelie N. An international collaborative study to establish the 1st international standard for HIV-1 RNA for use in nucleic acidbased techniques. J Virol Methods 2001, 92:141-150.
5. Jorgensen P.A., Neuwald P.D. Standardized hepatitis C virus RNA panels for nucleic acid testing assays. J Clin Virol 2001, 20:35-40.
6. Robertson J.S. International standardization of gene amplification technology. Biologicals 1998, 26:111-113.
7. Saldanha J., Lelie N., Heath A. Establishment of the first international standard for nucleic acid amplification technology (NAT) assays for HCV RNA. WHO Collaborative Study Group. Vox Sang 1999, 76:149-158.
8. Bakker N.A., Verschuuren E.A., Veeger N.J., van der Bij W., van Imhoff G.W., Kallenberg C.G., et al. Quantification of Epstein-Barr virus-DNA load in lung transplant recipients: a comparison of plasma versus whole blood. J Heart Lung Transplant 2008, 27:7-10.
9. Hakim H., Gibson C., Pan J., Srivastava K., Gu Z., Bankowski M.J., et al. Comparison of various blood compartments and reporting units for the detection and quantification of Epstein-Barr virus in peripheral blood. J Clin Microbiol 2007, 45:2151-2155.
10. Kullberg-Lindh C., Olofsson S., Brune M., Lindh M. Comparison of serum and whole blood levels of cytomegalovirus and Epstein-Barr virus DNA. Transplant Infect Dis 2008, 10:308-315.
11. Tsai D.E., Douglas L., Andreadis C., Vogl D.T., Arnoldi S., Kotloff R., et al. EBV PCR in the diagnosis and monitoring of posttransplant lymphoproliferative disorder: results of a two-arm prospective trial. Am J Transplant 2008, 8:1016-1024.
12. Wagner H.J., Wessel M., Jabs W., Smets F., Fischer L., Offner G., et al. Patients at risk for development of posttransplant lymphoproliferative disorder: plasma versus peripheral blood mononuclear cells as material for quantification of Epstein-Barr viral load by using real-time quantitative polymerase chain reaction. Transplantation 2001, 72:1012-1019.
13. Hayden R.T., Hokanson K.M., Pounds S.B., Bankowski M.J., Belzer S.W., Carr J., et al. Multicenter comparison of different real-time PCR assays for quantitative detection of Epstein-Barr virus. J Clin Microbiol 2008, 46:157-163.
14. Preiksaitis J.K., Pang X.L., Fox J.D., Fenton J.M., Caliendo A.M., Miller G.G. Interlaboratory comparison of Epstein-Barr virus viral load assays. Am J Transplant 2009, 9:269-279.
15. Abbate I., Zanchetta M., Gatti M., Gabrielli L., Zanussi S., Milia M.G., et al. Multicenter comparative study of Epstein-Barr virus DNA quantification for virological monitoring in transplanted patients. J Clin Virol 2011, 50:224-229.
16. Rowe D.T., Webber S., Schauer E.M., Reyes J., Green M. Epstein-Barr virus load monitoring: its role in the prevention and management of post-transplant lymphoproliferative disease. Transplant Infect Dis 2001, 3:79-87.
17. Pham L.V., Tamayo A.T., Yoshimura L.C., Lo P., Terry N., Reid P.S., et al. A CD40 signalosome anchored in lipid rafts leads to constitutive activation of NF-kappaB and autonomous cell growth in B cell lymphomas. Immunity 2002, 16:37-50.
18. Zhou H.J., Pham L.V., Tamayo A.T., Lin-Lee Y.C., Fu L., Yoshimura L.C., et al. Nuclear CD40 interacts with c-Rel and enhances proliferation in aggressive B-cell lymphoma. Blood 2007, 110:2121-2127.
19. Telenti A., Marshall W.F., Smith T.F. Detection of Epstein-Barr virus by polymerase chain reaction. J Clin Microbiol 1990, 28:2187-2190.
20. Riddler S.A., Breinig M.C., McKnight J.L. Increased levels of circulating Epstein-Barr virus (EBV)-infected lymphocytes and decreased EBV nuclear antigen antibody responses are associated with the development of posttransplant lymphoproliferative disease in solid-organ transplant recipients. Blood 1994, 84:972-984.
21. Stevens S.J., Vervoort M.B., van den Brule A.J., Meenhorst P.L., Meijer C.J., Middeldorp J.M. Monitoring of Epstein-Barr virus DNA load in peripheral blood by quantitative competitive PCR. J Clin Microbiol 1999, 37:2852-2857.
22. Wagner H.J., Jabs W., Smets F., Wessel M., Fischer L., Offner G., et al. Real-time polymerase chain reaction (RQ-PCR) fort he monitoring of Epstein-Barr virus (EBV) load in peripheral blood mononuclear cells. Klien Pädiatr 2000, 212:206-210.
23. Baldanti F., Gatti M., Furione M., Paolucci S., Tinelli C., Comoli P., et al. Kinetics of Epstein-Barr virus DNA load in different blood compartments of pediatric recipients of T-cell-depleted HLA-haploidentical stem cell transplantation. J Clin Microbiol 2008, 46:3672-3677.
24. Baer R., Bankier A.T., Biggin M.D., Deininger P.L., Farrell P.J., Gibson T.J., et al. DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 1984, 310:207-211.
25. Gärtner B., Preiksaitis J.K. EBV viral load detection in clinical virology. J Clin Virol 2010, 48:82-90.
26. Jabs W.J., Henning H., Kittel M., Pethig K., Smets F., Bucsky P., et al. Normalized quantification by real-time PCR of Epstein-Barr virus load in patients at risk for posttransplant lymphoproliferative disorders. J Clin Microbiol 2001, 39:564-569.
27. Babcock G.J., Decker L.L., Volke M., Thorley-Lawson D. EBV persistence in memory B cells in vivo. Immunity 1998, 9:395-404.
28. Wadowsky R.M., Laus S., Green M., Webber S.A., Rowe D. Measurement of Epstein-Barr virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR. J Clin Microbiol 2003, 41:5245-5249.
29. Katz B.Z., Pahl E., Crawford S.E., Kostyk M.C., Rodgers S., Seshadri R., et al. Case-control study of risk factor fort he development of post-transplant lymphoproliferative disease in a pediatric heart transplant cohort. Pediatr Transplant 2007, 11:58-65.
30. Kirk R., Edwards L.B., Aurora P., Taylor D.O., Christie J.D., Dobbels F., et al. Registry of the international society for heart and lung transplantation: twelfth official pediatric heart transplantation report-2009. J Heart Lung Transplant 2009, 28:993-1006.
31. Mendoza F., Kunitake H., Laks H., Odim J. Post-transplant lymphoproliferative disorder following pediatric heart transplantation. Pediatr Transplant 2006, 10:60-66.
32. Webber S.A., Naftel D., Fricker F.J., Olesnevich P., Blume E.D., Addonizio L., et al. Lymphoproliferative disorders after paediatric heart transplantation: a multiinstitutional study. Lancet 2006, 367:233-239.
33. Curtis R.E., Travis L.B., Rowlings P.A., Socié G., Kingma D.W., Banks PM Risk of lymphoproliferative disorders after bone marrow transplantation: a multi-institutional study. Blood 1999, 94:2208-2216.
34. Bingler M.A., Feingold B., Miller S.A., Quivers E., Michaels M.G., Green M., et al. Chronic high Epstein-Barr viral load state and risk for late-onset posttransplant lymphoproliferative disease/lymphoma in children. Am J Transplant 2008, 8:442-445.
35. Wagner H.J., Fischer L., Jabs W., Holbe M., Pethig K., Bucsky P. Longitudinal analysis of Epstein-Barr viral load in plasma and peripheral blood mononuclear cells of transplanted patients by real-time polymerase chain reaction. Transplantation 2002, 74:656-664.
36. Kinch A., Oberg G., Arvidson J., Falk K.I., Linde A., Pauksens K. Post-transplant lymphoproliferative disease and other Epstein-Barr virus diseases in allogeneic haematopoietic stem cell transplantation after introduction of monitoring of viral load by polymerase chain reaction. Scand J Infect Dis 2007, 39:235-244.
37. Meerbach A., Wutzler P., Häfer R., Zintl F., Gruhn B. Monitoring of Epstein-Barr virus load after hematopoietic stem cell transplantation for early intervention in post-transplant lymphoproliferative disease. J Med Virol 2008, 80:441-454.
38. Faye A., Quartier P., Reguerre Y., Lutz P., Carret A.S., Deheâe A., et al. Chimaeric anti-CD20 monoclonal antibody (rituximab) in post-transplant B-lymphoproliferative disorder following stem cell transplantation in children. Brit J Haematol 2001, 115:112-118.
39. Green M. Management of Epstein-Barr virus-induced posttransplant lymphoproliferative disease in recipients of solid organ transplantation. Am J Transplant 2001, 1:103-108.
40. Aalto S.M., Juvonen E., Tarkkanen J., Volin L., Ruutu T., Mattila P.S., et al. Lymphoproliferative disease after allogeneic stem cell transplantation - pre-emptive diagnosis by quantification of Epstein-Barr virus DNA in serum. J Clin Virol 2003, 28:275-283.
41. Greenfield H.M., Gharib M.I., Turner A.J.L., Guiver M., Carr T., Will A.M., et al. The impact of monitoring Epstein-Barr virus PCR in paediatric bone marrow transplant patients: can it successfully predict outcome and guide intervention?. Pediatr Blood Cancer 2006, 47:200-205.
42. Yang J., Tao Q., Flinn I.W., Murray P.G., Post L.E., Ma H., et al. Characterization of Epstein-Barr virus-infected B cells in patients with posttransplantation lymphoproliferative disease: disappearance after rituximab therapy does not predict clinical response. Blood 2000, 96:4055-4063.
43. Styczynski J., Reusser P., Einsele H., de la Camara R., Cordonnier C., Ward K.N., et al. Management of HSV, VZV and EBV infections in patients with hematological malignancies and after SCT: guidelines from the Second European Conference on Infections in Leukemia. Bone Marrow Transplant 2009, 43:757-770.
44. Wagner H.J., Cheng Y.C., Huls M.H., Gee A.P., Kuehnle I., Krance R.A., et al. Prompt versus preemptive intervention for EBV lymphoproliferative disease. Blood 2004, 103:3979-3981.