Investigation of the effect of pressure and liophilic mobile phase additives on retention of small molecules and proteins using reversed-phase ultrahigh pressure liquid chromatography

Journal of Liquid Chromatography and Related Technologies

Volumn 35, Issue 3, 2012, Pages 407-427

  Makarov, Alexey ^a   Lobrutto, Rosario ^a   Karpinski, Paul ^a   Kazakevich, Yuri ^b   Christodoulatos, Christos ^c   Ganguly, A K ^c  

^a NOVARTIS PHARMA AG   (Switzerland)

^b SETON HALL UNIVERSITY   (United States)

^c STEVENS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

HPLC; liophilic; pressure; proteins retention; UHPLC

Indexed keywords

ANALYTES; BACK PRESSURES; CONSTANT FLOW RATES; CONSTANT PRESSURES; CONSTANT TEMPERATURE; EFFECT OF PRESSURE; EFFECTIVE TOOL; FAST-LC; HEXAFLUOROPHOSPHATES; HIGH PRESSURE; HPLC; HPLC METHOD; ISOCRATIC MODES; LIOPHILIC; MOBILE PHASE; MOBILE PHASE ADDITIVES; NEUTRAL MOLECULES; PEAK EFFICIENCY; PRESSURE VARIATIONS; RETENTION FACTORS; REVERSED PHASE; REVERSED-PHASE LIQUID CHROMATOGRAPHY; SMALL MOLECULES; SODIUM PERCHLORATE; TRANSFERRING METHOD; UHPLC; ULTRAHIGH-PRESSURE LIQUID CHROMATOGRAPHIES;

EXPERIMENTS; IONIZATION; LIQUID CHROMATOGRAPHY; POTASSIUM; PRESSURE; PRESSURE EFFECTS;

PROTEINS;

AMITRIPTYLINE; APROTININ; BRADYKININ; CYTOCHROME C; INSULIN; LABETALOL; LYSOZYME; MYOGLOBIN; NAPHTHALENE; NORTRIPTYLINE; PEPTIDE; PROPYL PARABEN; PROTEIN; PYRIDINE; RIBONUCLEASE A; SODIUM PERCHLORATE; URACIL; UREA;

ARTICLE; FLOW RATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PARTICLE SIZE; PRESSURE; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TEMPERATURE; THERMODYNAMICS; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 84856937338     PISSN: 10826076     EISSN: 1520572X     Source Type: Journal    
DOI: 10.1080/10826076.2011.601494     Document Type: Article

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