Stem cell gene expression in MRPS18-2-immortalized rat embryonic fibroblasts

Cell Death and Disease

Volumn 3, Issue 1, 2012, Pages

  Yenamandra, S P ^a,e   Darekar, S D ^a   Kashuba, V ^a,b   Matskova, L ^c   Klein, G ^a   Kashuba, E ^a,d  

^a KAROLINSKA INSTITUTE   (Sweden)

^b INSTITUTE OF MOLECULAR BIOLOGY AND GENETICS   (Ukraine)

^c Ludwig Institute   (Sweden)

^d R E KAVETSKY INSTITUTE OF EXPERIMENTAL PATHOLOGY   (Ukraine)

^e BIOINFORMATICS INSTITUTE   (Singapore)

Author keywords

Immortalization; Mitochondrial ribosomal protein S18 2; Primary cells; Stem cell markers

Indexed keywords

FATTY ACID; KERATIN; KRUPPEL LIKE FACTOR 4; MITOCHONDRIAL RIBOSOMAL PROTEIN 18-2; MYC PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; PROTEIN KINASE B; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; PYRUVIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; RIBOSOME PROTEIN; STAGE SPECIFIC EMBRYO ANTIGEN 1; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2; UBIQUINONE; UNCLASSIFIED DRUG; VIMENTIN; CD15 ANTIGEN; NANOG PROTEIN, RAT; SOX2 PROTEIN, RAT; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR SOX;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIGEN EXPRESSION; ARTICLE; BIOSYNTHESIS; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CELL LINE; CELL PROLIFERATION; CONTROLLED STUDY; EMBRYONIC STEM CELL; FIBROBLAST; GENE EXPRESSION; HUMAN; INOCULATION; NONHUMAN; OXIDATION REDUCTION REACTION; OXIDATIVE PHOSPHORYLATION; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; RECEPTOR DOWN REGULATION; SEVERE COMBINED IMMUNODEFICIENCY; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION; TRANSLATION REGULATION; UPREGULATION; ANIMAL; CYTOLOGY; DNA MICROARRAY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; GENETICS; METABOLISM; MOUSE; MOUSE MUTANT; PLASMID; XENOGRAFT;

MUS; RATTUS;

ANIMALS; ANTIGENS, CD15; CELL DIFFERENTIATION; CELL LINE, TRANSFORMED; CELL PROLIFERATION; EMBRYONIC STEM CELLS; FIBROBLASTS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HUMANS; KERATINS; MICE; MICE, SCID; OCTAMER TRANSCRIPTION FACTOR-3; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PLASMIDS; RATS; RIBOSOMAL PROTEINS; SOXB1 TRANSCRIPTION FACTORS; TRANSCRIPTION FACTORS; TRANSFECTION; TRANSPLANTATION, HETEROLOGOUS;

EID: 84856854931     PISSN: None     EISSN: 20414889     Source Type: Journal    
DOI: 10.1038/cddis.2011.138     Document Type: Article

References (17)

1. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006; 126: 663-676. (Pubitemid 44233629)
2. Wernig M, Meissner A, Foreman R, Brambrink T, Ku M, Hochedlinger K et al. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 2007; 448: 318-324. (Pubitemid 47080337)
3. Meissner A, Wernig M, Jaenisch R. Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells. Nat Biotech 2007; 25: 1177-1181. (Pubitemid 47538112)
4. Blelloch R, Venere M, Yen J, Ramalho-Santos M. Generation of induced pluripotent stem cells in the absence of drug selection. Cell Stem Cell 2007; 1: 245-247. (Pubitemid 47355331)
5. Liao J, Cui C, Chen S, Ren J, Chen J, Gao Y et al. Generation of induced pluripotent stem cell lines from adult rat cells. Cell Stem Cell 2009; 4: 11-15.
6. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007; 318: 1917-1920.
7. Huangfu D, Osafune K, Maehr R, Guo W, Eijkelenboom A, Chen S et al. Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nat Biotech 2008; 26: 1269-1275.
8. Giorgetti A, Montserrat N, Aasen T, Gonzalez F, Rodriguez-Piza I, Vassena R et al. Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2. Cell Stem Cell 2009; 5: 353-357.
9. Giorgetti A, Montserrat N, Rodriguez-Piza I, Azqueta C, Veiga A, Izpisua Belmonte JC. Generation of induced pluripotent stem cells from human cord blood cells with only two factors: Oct4 and Sox2. Nat Prot 2010; 5: 811-820.
10. Kashuba E, Pavan Yenamandra S, Deoram Darekar S, Yurchenko M, Kashuba V, Klein G et al. MRPS18-2 protein immortalizes primary rat embryonic fibroblasts and endows them with stem cell-like properties. Proc Nat Acad Sci 2009; 106: 19866-19871.
11. Chen GY, Osada H, Santamaria-Babi LF, Kannagi R. Interaction of GATA-3/T-bet transcription factors regulates expression of sialyl Lewis X homing receptors on Th1/Th2 lymphocytes. Proc Nat Acad Sci 2006; 103: 16894-16899. (Pubitemid 44737349)
12. Hao Z, Jha KN, Kim YH, Vemuganti S, Westbrook VA, Chertihin O et al. Expression analysis of the human testis-specific serine/threonine kinase (TSSK) homologues. A TSSK member is present in the equatorial segment of human sperm. Mol Hum Reprod 2004; 10: 433-444. (Pubitemid 38714376)
13. Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ et al. Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome Res 2000; 10: 1546-1560.
14. Suzuki T, Terasaki M, Takemoto-Hori C, Hanada T, Ueda T, Wada A et al. Proteomic analysis of the mammalian mitochondrial ribosome. Identification of protein components in the 28 S small subunit. J Biol Chem 2001; 276: 33181-33195.
15. Cavdar Koc E, Burkhart W, Blackburn K, Moseley A, Spremulli LL. The small subunit of the mammalian mitochondrial ribosome. Identification of the full complement of ribosomal proteins present. J Biol Chem 2001; 276: 19363-19374.
16. Snopok B, Yurchenko M, Szekely L, Klein G, Kashuba E. SPR-based immunocapture approach to creating an interfacial sensing architecture: mapping of the MRS18-2 binding site on retinoblastoma protein. Anal Bioanal Chem 2006; 386: 2063-2073.
17. Kashuba E, Yurchenko M, Yenamandra SP, Snopok B, Isaguliants M, Szekely L et al. EBV-encoded EBNA-6 binds and targets MRS18-2 to the nucleus, resulting in the disruption of pRb-E2F1 complexes. Proc Nat Acad Sci 2008; 105: 5489-5494. (Pubitemid 351753890)