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Volumn 58, Issue 1, 2012, Pages 59-64

Use of a ura5 +-lys7 + cassette to construct unmarked gene knock-ins in Schizosaccharomyces pombe

Author keywords

Fission yeast; Gene knock in; lys7 +; Schizosaccharomyces pombe; Selectable marker; ura5 +

Indexed keywords

ARTICLE; CONTROLLED STUDY; FUNGAL GENE; FUNGAL GENOME; FUNGAL STRAIN; FUNGUS GROWTH; GENE CASSETTE; GENE MUTATION; GENE TARGETING; HOMOLOGOUS RECOMBINATION; NONHUMAN; OROTATE PHOSPHORIBOSYLTRANSFERASE GENE; PRIORITY JOURNAL; SCHIZOSACCHAROMYCES POMBE; SCK1 KINASE GENE; URA4 GENE; URA5 GENE; URA5 LYS7 GENE;

EID: 84856823960     PISSN: 01728083     EISSN: 14320983     Source Type: Journal    
DOI: 10.1007/s00294-011-0360-4     Document Type: Article
Times cited : (16)

References (11)
  • 1
    • 0031818471 scopus 로고    scopus 로고
    • Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe
    • DOI 10.1002/(SICI)1097-0061(199807)14:10<943::AID-YEA292>3.0.CO;2-Y
    • J Bahler JQ Wu MS Longtine NG Shah A McKenzie 3rd AB Steever A Wach P Philippsen JR Pringle 1998 Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe Yeast 14 10 943 951 9717240 10.1002/(SICI)1097-0061(199807)14:10<943::AID-YEA292>3.0.CO;2-Y 1:CAS:528:DyaK1cXltVentLs%3D (Pubitemid 28328000)
    • (1998) Yeast , vol.14 , Issue.10 , pp. 943-951
    • Bahler, J.1    Wu, J.-Q.2    Longtine, M.S.3    Shah, N.G.4    McKenzie III, A.5    Steever, A.B.6    Wach, A.7    Philippsen, P.8    Pringle, J.R.9
  • 2
    • 0023484186 scopus 로고
    • 5-Fluoroorotic acid as a selective agent in yeast molecular genetics
    • 3323810 10.1016/0076-6879(87)54076-9 1:CAS:528:DyaL1cXktVenur4%3D
    • JD Boeke J Trueheart G Natsoulis GR Fink 1987 5-Fluoroorotic acid as a selective agent in yeast molecular genetics Methods Enzymol 154 164 175 3323810 10.1016/0076-6879(87)54076-9 1:CAS:528:DyaL1cXktVenur4%3D
    • (1987) Methods Enzymol , vol.154 , pp. 164-175
    • Boeke, J.D.1    Trueheart, J.2    Natsoulis, G.3    Fink, G.R.4
  • 3
    • 0024556721 scopus 로고
    • Structure and expression of the URA5 gene of Saccharomyces cerevisiae
    • DOI 10.1007/BF00427043
    • J de Montigny A Belarbi JC Hubert F Lacroute 1989 Structure and expression of the URA5 gene of Saccharomyces cerevisiae Mol Gen Genet 215 3 455 462 2651891 10.1007/BF00427043 (Pubitemid 19061474)
    • (1989) Molecular and General Genetics , vol.215 , Issue.3 , pp. 455-462
    • De Montigny, J.1    Belarbi, A.2    Hubert, J.-C.3    Lacroute, F.4
  • 4
    • 0025355324 scopus 로고
    • Cloning and sequencing of URA10, a second gene encoding orotate phosphoribosyl transferase in Saccharomyces cerevisiae
    • J de Montigny L Kern JC Hubert F Lacroute 1990 Cloning and sequencing of URA10, a second gene encoding orotate phosphoribosyl transferase in Saccharomyces cerevisiae Curr Genet 17 2 105 111 2182197 10.1007/BF00312853 (Pubitemid 20101184)
    • (1990) Current Genetics , vol.17 , Issue.2 , pp. 105-111
    • De Montigny, J.1    Kern, L.2    Hubert, J.-C.3    Lacroute, F.4
  • 6
    • 17844378717 scopus 로고    scopus 로고
    • Direct activation of fission yeast adenylate cyclase by the Gpa2 Gα of the glucose signaling pathway
    • DOI 10.1073/pnas.0502270102
    • FD Ivey CS Hoffman 2005 Direct activation of fission yeast adenylate cyclase by the Gpa2 Gα of the glucose signaling pathway Proc Natl Acad Sci USA 102 17 6108 6113 15831585 10.1073/pnas.0502270102 1:CAS:528: DC%2BD2MXkt1Clsrc%3D (Pubitemid 40594263)
    • (2005) Proceedings of the National Academy of Sciences of the United States of America , vol.102 , Issue.17 , pp. 6108-6113
    • Ivey, F.D.1    Hoffman, C.S.2
  • 7
    • 77950539110 scopus 로고    scopus 로고
    • + Gα gene identify residues involved in GDP-GTP exchange
    • 10.1128/EC.00010-10 20139237 10.1128/EC.00010-10 1:CAS:528: DC%2BC3cXks1Ojtrg%3D
    • + Gα gene identify residues involved in GDP-GTP exchange Eukaryot Cell 9 4 626 633 10.1128/EC.00010-10 20139237 10.1128/EC.00010-10 1:CAS:528:DC%2BC3cXks1Ojtrg%3D
    • (2010) Eukaryot Cell , vol.9 , Issue.4 , pp. 626-633
    • Ivey, F.D.1    Taglia, F.X.2    Yang, F.3    Lander, M.M.4    Kelly, D.A.5    Hoffman, C.S.6
  • 8
    • 0035103407 scopus 로고    scopus 로고
    • Transcriptional regulators of the Schizosaccharomyces pombe fbp1 gene include two redundant Tup1p-like corepressors and the CCAAT binding factor activation complex
    • RT Janoo LA Neely BR Braun SK Whitehall CS Hoffman 2001 Transcriptional regulators of the Schizosaccharomyces pombe fbp1 gene include two redundant Tup1p-like corepressors and the CCAAT binding factor activation complex Genetics 157 3 1205 1215 11238405 1:CAS:528:DC%2BD3MXisFyktL0%3D (Pubitemid 32225013)
    • (2001) Genetics , vol.157 , Issue.3 , pp. 1205-1215
    • Janoo, R.T.K.1    Neely, L.A.2    Braun, B.R.3    Whitehall, S.K.4    Hoffman, C.S.5
  • 9
    • 0029012096 scopus 로고
    • Sck1, a high copy number suppressor of defects in the cAMP-dependent protein kinase pathway in fission yeast, encodes a protein homologous to the Saccharomyces cerevisiae SCH9 kinase
    • 7498728 1:CAS:528:DyaK28Xht1Cgsro%3D
    • M Jin M Fujita BM Culley E Apolinario M Yamamoto K Maundrell CS Hoffman 1995 sck1, a high copy number suppressor of defects in the cAMP-dependent protein kinase pathway in fission yeast, encodes a protein homologous to the Saccharomyces cerevisiae SCH9 kinase Genetics 140 2 457 467 7498728 1:CAS:528:DyaK28Xht1Cgsro%3D
    • (1995) Genetics , vol.140 , Issue.2 , pp. 457-467
    • Jin, M.1    Fujita, M.2    Culley, B.M.3    Apolinario, E.4    Yamamoto, M.5    Maundrell, K.6    Hoffman, C.S.7
  • 10
    • 0034903337 scopus 로고    scopus 로고
    • In vivo site-directed mutagenesis using oligonucleotides
    • DOI 10.1038/90837
    • F Storici LK Lewis MA Resnick 2001 In vivo site-directed mutagenesis using oligonucleotides Nat Biotechnol 19 8 773 776 10.1038/90837 11479573 10.1038/90837 1:CAS:528:DC%2BD3MXlslektLg%3D (Pubitemid 32729542)
    • (2001) Nature Biotechnology , vol.19 , Issue.8 , pp. 773-776
    • Storici, F.1    Lewis, L.K.2    Resnick, M.A.3
  • 11
    • 2442505374 scopus 로고    scopus 로고
    • Strategies for gene disruptions and plasmid constructions in fission yeast
    • DOI 10.1016/j.ymeth.2003.11.014, PII S104620230300313X
    • L Wang R Kao FD Ivey CS Hoffman 2004 Strategies for gene disruptions and plasmid constructions in fission yeast Methods 33 199 205 15157886 10.1016/j.ymeth.2003.11.014 1:CAS:528:DC%2BD2cXkt1CmsLo%3D (Pubitemid 38648840)
    • (2004) Methods , vol.33 , Issue.3 , pp. 199-205
    • Wang, L.1    Kao, R.2    Ivey, F.D.3    Hoffman, C.S.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.