The concurrent use of N-and C-terminal antibodies anti-nucleophosmin 1 in immunofluorescence experiments allows for precise assessment of its subcellular localisation in acute myeloid leukaemia patients

Leukemia

Volumn 26, Issue 1, 2012, Pages 159-162

  Gruszka, A M ^a   Martinelli, C ^a   Sparacio, E ^a   Pelicci, P G ^a,b   De Marco, A ^a,c  

^a EUROPEAN INSTITUTE OF ONCOLOGY   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

^c UNIVERSITY OF NOVA GORICA   (Slovenia)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; NUCLEOPHOSMIN; NUCLEOPHOSMIN ANTIBODY; UNCLASSIFIED DRUG;

ACUTE GRANULOCYTIC LEUKEMIA; AMINO TERMINAL SEQUENCE; CANCER PATIENT; CARBOXY TERMINAL SEQUENCE; CYTOPLASM; FLOW CYTOMETRY; GENE LOCUS; GENE MUTATION; HUMAN; IMMUNOFLUORESCENCE TEST; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; LETTER; NUCLEOLUS; PRIORITY JOURNAL; WESTERN BLOTTING;

EID: 84856734081     PISSN: 08876924     EISSN: 14765551     Source Type: Journal    
DOI: 10.1038/leu.2011.177     Document Type: Letter

References (15)

1. Falini B, Nicoletti I, Martelli MF, Mecucci C. Acute myeloid leukemia carrying cytoplasmic/mutated nucleophosmin (NPMc+ AML): biological and clinical features. Blood 2007; 109: 874-885. (Pubitemid 46220630)
2. Falini B, Mecucci C, Tiacci E, Alcalay M, Rosati R, Pasqualucci L et al. Cytoplasmic nucleophosmin in acute myelogenous leukemia with a normal karyotype. N Engl J Med 2005; 352: 254-266. (Pubitemid 40110814)
3. Oelschlaegel U, Koch S, Mohr B, Schaich M, Falini B, Ehninger G et al. Rapid flow cytometric detection of aberrant cytoplasmic localization of nucleophosmin (NPMc) indicating mutant NPM1 gene in acute myeloid leukemia. Leukemia 2010; 24: 1813-1816.
4. Cordell JL, Pulford KAF, Bigerna B, Roncador G, Banham A, Colombo E et al. Detection of normal and chimeric nucleophosmin in human cells. Blood 1999; 93: 632-642. (Pubitemid 29035746)
5. Falini B, Martelli MP, Bolli N, Bonasso R, Ghia E, Pallotta MT et al. Immunohistochemistry predicts nucleophosmin (NPM) mutations in acute myeloid leukemia. Blood 2006; 108: 1999-2005. (Pubitemid 44395013)
6. Luo J, Qi C, Xu W, Kamel-Reid S, Brandwein J, Chang H. Cytoplasmic expression of nucleophosmin accurately predicts mutation in the nucleophosmin gene in patients with acute myeloid leukemia and normal karyotype. Am J Clin Pathol 2010; 133: 34-40.
7. Quentmeier H, Martelli MP, Dirks WG, Bolli N, Liso A, MacLeod RAF et al. Cell line OCI/AML3 bears exon-12 gene mutation-A and cytoplasmic expression of nucleophosmin. Leukemia 2005; 19: 1760-1767. (Pubitemid 41486153)
8. Martelli MP, Manes N, Liso A, Pettirossi V, Verducci Galletti B, Bigerna B et al. A western blot assay for detecting mutant nucleophosmin (NPM1) proteins in acute myeloid leukaemia. Leukemia 2008; 22: 2285-2288.
9. Gruszka AM, Lavorgna S, Consalvo MI, Ottone T, Martinelli C, Cinquanta M et al. A monoclonal antibody against mutated nucleophosmin1 for the molecular diagnosis of acute myeloid leukemias. Blood 2010; 116: 2096-2102.
10. Colombo E, Martinelli P, Zamponi R, Shing DC, Bonetti P, Luzi L et al. Delocalization and destabilization of the Arf tumor suppressor by the leukemia-associated NPM mutant. Cancer Res 2006; 66: 3044-3050.
11. Bolli N, De Marco MF, Martelli MP, Bigerna B, Pucciarini A, Rossi R et al. A dose-dependent tug of war involving the NPM1 leukaemic mutant, nucleophosmin, and ARF. Leukemia 2009; 23: 501-509.
12. Konoplev S, Huang X, Drabkin HA, Koeppen H, Jones D, Kantarjian HM et al. Cytoplasmic localization of nucleophosmin in bone marrow blasts of acute myeloid leukemia patients is not completely concordant with NPM1 mutation and is not predictive of prognosis. Cancer 2009; 115: 4737-4744.
13. Mattsson G, Turner SH, Cordell J, Ferguson DJP, Schuh A, Grimwade LF et al. Can cytoplasmic nucleophosmin be detected by immunocytochemical staining of cell smears in acute myeloid leukemia? Haematologica 2010; 95: 670-673.
14. Monegal A, Ami D, Martinelli C, Huang H, Aliprandi M, Capasso P et al. Immunological applications of single domain llama recombinant antibodies isolated from a na?̈ve library. Prot Engineer Des Sel 2009; 22: 273-280.
15. Aliprandi M, Sparacio E, Pivetta F, Ossolengo G, Maestro R, de Marco A. The availability of a recombinant anti-SNAP antibody in VHH format amplifies the application flexibility of SNAP-tagged proteins. J Biomed Biotechnol 2010; 2010: 658954.