Generation of 2A-linked multicistronic cassettes by recombinant PCR

Cold Spring Harbor Protocols

Volumn 7, Issue 2, 2012, Pages 251-254

  Szymczak Workman, Andrea L ^a   Vignali, Kate M ^a   Vignali, Dario A A ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; EXPRESSION VECTOR; GEL ELECTROPHORESIS; GENE CASSETTE; GENE DELIVERY SYSTEM; GENE EXPRESSION; HOMOLOGOUS RECOMBINATION; POLYMERASE CHAIN REACTION; PROTEIN CLEAVAGE; QUANTITATIVE ANALYSIS; STOICHIOMETRY; GENE; GENE VECTOR; GENETIC ENGINEERING; GENETICS; METABOLISM; METHODOLOGY; OPEN READING FRAME; PROTEIN DEGRADATION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; RIBOSOME;

PEPTIDE;

GENE EXPRESSION; GENES; GENETIC ENGINEERING; GENETIC VECTORS; OPEN READING FRAMES; PEPTIDES; POLYMERASE CHAIN REACTION; PROTEIN BIOSYNTHESIS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEOLYSIS; RIBOSOMES;

EID: 84856460886     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot067884     Document Type: Article

References (6)

1. Baskaran N, Kandpal RP, Bhargava AK, Glynn MW, Bale A, Weissman SM. 1996. Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content. Genome Res 6: 633-638.
2. Henke W, Herdel K, Jung K, Schnorr D, Loening SA. 1997. Betaine improves the PCR amplification of GC-rich DNA sequences. Nucleic Acids Res 25: 3957-3958.
3. Rees WA, Yager TD, Korte J, von Hippel PH. 1993. Betaine can eliminate the base pair composition dependence of DNA melting. Biochemistry 32: 137-144.
4. Szymczak-Workman AL, Vignali KM, Vignali DAA. 2012a. Design and construction of 2A peptide-linked multicistronic vectors. Cold Spring Harb Protoc doi: 10.1101/pdb.ip067876.
5. Szymczak-Workman AL, Vignali KM, Vignali DAA. 2012b. Verification of 2A peptide cleavage. Cold Spring Harb Protoc doi: 10.1101/pdb. prot067892.
6. Varadaraj K, Skinner DM. 1994. Denaturants or cosolvents improve the specificity of PCR amplification of a G+C-rich DNA using genetically engineered DNA polymerases. Gene 140: 1-5.