Verification of 2A peptide cleavage

Cold Spring Harbor Protocols

Volumn 7, Issue 2, 2012, Pages 255-257

  Szymczak Workman, Andrea L ^a   Vignali, Kate M ^a   Vignali, Dario A A ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; ANTIBODY DETECTION; ARTICLE; CONTROLLED STUDY; EMBRYO; EMBRYO CELL; GENE TARGETING; HUMAN; HUMAN CELL; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; STOICHIOMETRY; WESTERN BLOTTING; CELL LINE; GENE; GENE EXPRESSION; GENE VECTOR; GENETIC ENGINEERING; GENETIC TRANSFECTION; GENETICS; METABOLISM; METHODOLOGY; PROTEIN DEGRADATION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; RIBOSOME;

PEPTIDE;

BLOTTING, WESTERN; CELL LINE; GENE EXPRESSION; GENES; GENETIC ENGINEERING; GENETIC VECTORS; HUMANS; OPEN READING FRAMES; PEPTIDES; PROTEIN BIOSYNTHESIS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEOLYSIS; RIBOSOMES; TRANSFECTION;

EID: 84856454058     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot067892     Document Type: Article

References (4)

1. Harlow E, Lane D. 1999. Using antibodies: A laboratory manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
2. Szymczak AL, Workman CJ, Wang Y, Vignali KM, Dilioglou S, Vanin EF, Vignali DA. 2004. Correction of multi-gene deficiency in vivo using a single "self-cleaving" 2A peptide-based retroviral vector. Nat Biotechnol 22: 589-594.
3. Szymczak-Workman AL, Vignali KM, Vignali DAA. 2012a. Design and construction of 2A peptide-linked multicistronic vectors. Cold Spring Harb Protoc doi: 10.1101/pdb.ip067876.
4. Szymczak-Workman AL, Vignali KM, Vignali DAA. 2012b. Generation of 2A-linked multicistronic cassettes by recombinant PCR. Cold Spring Harb Protoc doi: 10.1101/pdb.prot067884.