Kinetic anomalies in the interactions of Nile red with microalgae

Journal of Microbiological Methods

Volumn 88, Issue 2, 2012, Pages 189-196

  Pick, Uri ^a   Rachutin Zalogin, Tatyana ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

Fluorescence; Kinetic anomalies; Lipid globules; Microalgae; Nile red

Indexed keywords

FLUORESCENT DYE; GLYCEROL; IODIDE; NILE RED; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

ARTICLE; CELL MEMBRANE; CELL ORGANELLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DUNALIELLA BARDAWIL; DUNALIELLA PARVA; DUNALIELLA SALINA; FLUORESCENCE; FLUORESCENCE QUENCHING; KINETICS; LIPID GLOBULE; LIPID STORAGE; MICROALGA; NANOCHLORIS ATOMUS; NONHUMAN; PRIORITY JOURNAL;

CELL MEMBRANE; FLUORESCENT DYES; GLYCEROL; KINETICS; LIPOSOMES; MICROALGAE; OXAZINES; PHOSPHATIDYLCHOLINES; SPECTROMETRY, FLUORESCENCE; TRIOLEIN;

ALGAE;

EID: 84856378559     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.10.008     Document Type: Article

References (19)

1. Ben-Amotz A., Katz A., Avron M. Accumulation of β-carotene in halotolerant algae: purification and characterization of β-carotene-rich globules from Dunaliella bardawil (CHLOROPYCEAE). J. Phycol. 1982, 18:529-537.
2. Ben-Amotz A., Shaish A., Avron M. Mode of action of the massively accumulated β-carotene of Dunaliella bardawil in protecting the alga against damage of access irradiation. Plant Physiol. 1989, 91:1040-1043.
3. Chen W., Zhang C., Song L., Sommerfeld M., Hu Q. A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae. J. Microbiol. Methods 2009, 77:41-47.
4. Chisty Y. Biodiesel from microalgae. Biotechnol. Adv. 2007, 25:294-306.
5. Coocksey K.E., Guckert J.B., Williams S.A., Callis P.A. Fluorimetric determination of neutral lipid content in microalgal cells using Nile red. J. Microbiol. Methods 1987, 6:333-345.
6. Deamer D.W., Prince R.C., Crofts A.R. The response of fluorescent amines to pH gradients across liposome membranes. Biochim. Biophys. Acta 1972, 274:323-335.
7. Elsey D., Jameson D., Raleigh B., Cooney M.J. Fluorescent measurement of microalgal neutral lipids. J. Microbiol. Methods 2007, 68:639-642.
8. Epps D.E., Sarver R.W., Rogers J.M., Herberg J.T., Tomich P.K. The ligand affinity of proteins measured by isothermal denaturation kinetics. Anal. Biochem. 2001, 292:40-50.
9. Greenspan P., Fowler S.D. Spectroscopic studies of a lipid probe, Nile red. J. Lipid Res. 1985, 26:781-789.
10. Katz A., Jiménez C., Pick U. Isolation and characterization of a protein which is associated with carotene globules in the alga Dunaliella bardawil. Plant Physiol. 1995, 108:1657-1664.
11. Katz A., Waridel P., Shevchenko A., Pick U. Salt-induced changes in the plasma membrane proteome of the halotolerant alga Dunaliella salina as revealed by Blue-Native gel electrophoresis and nano LC-MS/MS analysis. Mol. Cell. Proteomics 2007, 6:1459-1472.
12. Kimura K., Yamaoka M., Kamisaka Y. Rapid estimation of lipids in oleaginous fungi and yeast using Nile red fluorescence. J. Microbiol. Methods 2004, 56:331-338.
13. Kraayenhof R. Quenching of uncoupler fluorescence in relation to the "energized state" of chloroplasts. FEBS Lett. 1970, 6:161-165.
14. Krishnamoorthy I.G. Fluorescence lifetime distribution in characterizing membrane heterogeneity. J. Fluoresc. 2001, 11:247-253.
15. Mukherjee S., Raghuraman H., Chattopadhyay A. Membrane localization and dynamics of Nile Red: effect of cholesterol. Biochim. Biophys. Acta 2007, 1768:59-66.
16. Pick U. Adaptation of the halotolerant alga Dunaliella to high salinity. Salinity: Environment-Plants-Molecules 2002, 97-112. Kluwer Academical, Dordrecht, the Netherlands. A. Läuchli, U. Lüettge (Eds.).
17. Pick U., MacCarty R.E. Measurement of membrane ΔpH. Methods in Enzymol 1980, 69:538-549. A. San-Pietro (Ed.).
18. Sackett D.L., Wolf J. Nile-Red as polarity sensitive probe of hydrophobic protein surfaces. Anal. Biochem. 1987, 167:228-234.
19. Shaish A., Avron M., Pick U., Ben-Amotz A. Are active oxygen species involved in induction of β-carotene in Dunaliella bardawil. Planta 1993, 190:362-368.